| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 337,836 | C→T | 21.7% | intergenic (‑86/+43) | PP_0279 ← / ← PP_0280 | hypothetical protein/amino acid ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 337,836 | 0 | C | T | 21.7% | 41.0 / 6.7 | 23 | intergenic (‑86/+43) | PP_0279/PP_0280 | hypothetical protein/amino acid ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (11/7); new base T (2/3); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
GGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAA > NC_002947/337690‑337980 | ggAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTacac < 7:119600/149‑1 (MQ=255) gTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTgg < 7:190321/148‑1 (MQ=255) gTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGGGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATG‑TGGCGCTATACCTGTgg < 6:320164/148‑1 (MQ=21) gCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGc < 3:264866/149‑1 (MQ=255) gCCTGGAAGCGGGGGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATG‑GGGCGCTATACCTGTGGGAGCGGGc < 5:64488/149‑1 (MQ=21) tGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCt > 3:64051/1‑91 (MQ=255) tGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCt < 4:64051/91‑1 (MQ=255) gcgGGCCCGCCCGCTCCCACTGGTATCGCGCC‑ACATTCATGGTTGCCGCTACACGTGTGGGAGCGGGCGGGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCcag < 3:167206/136‑1 (MQ=255) aCGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCAc > 2:357378/1‑148 (MQ=255) cGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCCTCCACGCGCAACCAGCGCGCCAGCAGCTTGAAGCCGTGCAc > 8:309419/1‑147 (MQ=255) cGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCAt > 6:30370/1‑149 (MQ=255) ccACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc > 4:322828/1‑148 (MQ=255) cacaGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc > 5:345847/1‑147 (MQ=255) cacaGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACga < 5:30370/149‑1 (MQ=255) cacaGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAAAAGCCAATGGAAGGAAACCCCCCGCAACCAGCGGACCAGCGGATTGAAGCCTTGCACCGGCATGAAc > 1:122303/1‑148 (MQ=25) acaGGTGTAGCGCCAACCTTGAATG‑TGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc > 3:211450/1‑146 (MQ=25) ataGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCTGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCAc > 8:80547/1‑148 (MQ=255) ttCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAacac < 7:218224/149‑1 (MQ=255) tCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACg > 1:272307/1‑149 (MQ=255) aaGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGcc > 1:227556/1‑149 (MQ=255) gtGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTCCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTgcg > 2:257929/2‑149 (MQ=255) gcgcTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTgcggc < 2:272307/148‑1 (MQ=255) gcgcTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTgcggc > 2:159475/1‑148 (MQ=255) gcTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCgaa < 7:191806/149‑1 (MQ=255) cacCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCgaa > 5:88397/1‑145 (MQ=255) cacCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCgaa < 6:88397/145‑1 (MQ=255) | GGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCC‑ACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCC‑TTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACACGCCTGCGGCGAA > NC_002947/337690‑337980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |