| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 95.8% | 75.4 / ‑2.8 | 24 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (11/12); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
CTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGC‑GGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑C‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCAC > NC_002947/1932500‑1932775 | cTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGC‑GGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAg < 2:28416/148‑1 (MQ=255) ttCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGC‑GGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCACGGCCGCCCCaaa > 3:22394/1‑148 (MQ=255) tgctcccgCGGCTTTGCCGGGCCCACCAAAATCCCATTAACCGGGGGGGCCG‑TTTGCGGGCCCTGCGCGGGCACGCCCGCTCCTAAAACGCATTGCCGGCCCCTGTCGGCGGGGGTTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCaaa < 8:153432/141‑1 (MQ=255) cAGCGGCCTGGCAGGGCGCACGAAAATAGCAGCAACCGCTGGGGCCGCTTGGC‑GGCCCTGCGCGGGCAAGCCCGCTCCTACAACGGATATGCGGCCCCGGTCGGCGCGGGCTGGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 7:38736/148‑1 (MQ=255) aGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGC‑GGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGAt < 3:45687/148‑1 (MQ=255) tctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGC‑GGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATGCGTCCCCTGTAGGAGCGGGCGTGCC‑CGCGAAGGGCTGCCAGGCAGCCCCAAATTGTCGGCTACGCGGATCAg > 7:97615/1‑148 (MQ=38) tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGC‑GGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCg > 4:80227/1‑148 (MQ=255) gAAAATAGCAGTAAACGCTGGGGCCGCTTTGC‑GGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGCGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCCGCCCCAAATTGTCGACTACGCGGATCAGCGAtac > 6:46160/1‑146 (MQ=255) aaaTAGCAGTAAACGCTGGGGCCGCTTTGC‑GGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCgg > 7:108146/1‑148 (MQ=255) aTAGCAGTAAACGCTGGGGCCGCTTTGC‑GGCCCTTCGCGGGCAAGCCCGCACCGACAAAGGATATTCGTGCCCGGTAGGAGCGGGCGTGCC‑CGCGCAGGGCTGGAAGGCCGCCCCAAATTGTCGGCAACGCGGATCAGCGCTGCGGcc > 7:79095/1‑147 (MQ=25) cgCAGTAAACGCGGGGGCCGCTTTGC‑GGCCCTTCGCGGGCAACCCCGCGCCTACAACGCATCTGCGCCCCCTGTAGGAGCGGGGTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 7:11963/147‑1 (MQ=38) gCAGTAAACGCTGGGGCCGCTTTGC‑GGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAggg < 2:15670/148‑1 (MQ=255) cGCTGGGGCCGCTTTGC‑GGCCCTGCGCGGGCACGCCCGCTCCTACAACGGATAGGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc < 8:54636/148‑1 (MQ=38) tCGCGGGCAAGCCCGCTCCTACAAAGGATATGGGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGCAGGGCTGCAAGGCCGCCCCAAATTGGCGCCTCCGCGGATCAGCGCTCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGcc > 6:36738/1‑148 (MQ=38) cgcgGGCAACCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGccc < 2:28498/148‑1 (MQ=255) gCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc < 8:108146/148‑1 (MQ=255) cGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGGAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGc > 6:37775/1‑148 (MQ=255) cAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGCGCAGCTGCCCTGGTTTTCCAGCTCGatat > 2:201883/1‑147 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc > 3:47475/1‑148 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CAAGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg > 6:106292/1‑147 (MQ=255) gTCCCCTGTCGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt < 8:79095/148‑1 (MQ=255) gTCCCCTGTAGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt > 8:79990/1‑148 (MQ=255) aGGAGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt < 7:46832/148‑1 (MQ=255) aGCGGGCTTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcac < 5:106292/148‑1 (MQ=255) | CTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGC‑GGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑C‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCAC > NC_002947/1932500‑1932775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |