| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,586,057 | +C | intergenic (+140/+75) | PP_4061 → / ← PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,586,056 | 1 | . | C | 85.7% | 34.6 / 1.2 | 14 | intergenic (+139/+76) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (5/7); total (7/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01 | |||||||||||
GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TCTGC‑GGTAAACCCGCTCCTACCA‑GGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG > NC_002947/4585918‑4586198 | ggggAGACCACGGCACCGGCGGGAACAGCTGCTCTGCGCTGCTCCATTGAAAGGGTGAGGATGACCGGGGGCTGGGCACGGGGACGCGCGTGACTGGCTCGGGCCGGCCGCGTCGCG‑GGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCg < 1:131751/148‑1 (MQ=255) aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCtt < 8:62460/147‑1 (MQ=39) cACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGaa < 5:141793/148‑1 (MQ=39) gCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa < 4:188551/148‑1 (MQ=39) gcgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTTAAACCCCTCCGAAAAGGGG‑C‑CCGGGCGGGCGTGAATGCGGCGCGGCCCCCGGGTGGCGCggg > 3:52534/1‑148 (MQ=255) gctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGc < 8:179048/143‑1 (MQ=38) gctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg < 5:147953/145‑1 (MQ=38) gtggcggATGACCGGGTGCTTGCCACGGTGACTCTCTTGCATTGATCGGGCCGGCCTC‑TGCGCGGGTAACCCCGCTCCACCCC‑GGG‑ACCGGGCCGGCCTGGAATGCGGCGCGGCCCCGGGTAGGAGCGGGTTTACCCGCGAAGAggcc < 8:105729/143‑1 (MQ=19) aCCCGCTCCTACCA‑GGGCC‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTCCCCGCGAAGCGGCCGGCCGCGCCCGCACATCAACCCGCCGATGCAGCCGCCCTCTCCGCCCCACTGAGCTCACGCCTGCGGaa > 4:81612/1‑148 (MQ=255) ccGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCACTCTCCGCCACCCTGATCTCCCGCATGCGGAACt > 2:148803/1‑148 (MQ=39) ccGCTCCTACCA‑GGC‑ACCGTGCCGGCCTTGAATGCGGCGCGGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGACGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAAGCTCCGCCCCACTGATCTCACGCATGCGGCACt > 2:146065/1‑148 (MQ=38) tACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGCGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTGCTGCAc > 1:114315/1‑148 (MQ=255) cA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCtt > 5:2361/1‑148 (MQ=255) a‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCCCCtgg > 7:162148/1‑146 (MQ=255) | GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TCTGC‑GGTAAACCCGCTCCTACCA‑GGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG > NC_002947/4585918‑4586198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |