Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,580,735	C→T	G221D (GGC→GAC)	ttgB ←	efflux pump membrane protein TtgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,580,735	0	C	T	100.0%	50.2 / NA	18	G221D (GGC→GAC)	ttgB	efflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/9);  total (9/9)

CGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTCGGCGGTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCAC  >  NC_002947/1580607‑1580861                                                                                                                                 |                                                                                                                               cGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTCGGCGGTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAg                                                                                                              >  3:69904/1‑147 (MQ=255)                                                 tctcGAACTGCTCGGCGGTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCgggg                                                              >  5:35124/1‑147 (MQ=255)                                                 tctcGAACTGCTCGGCGGTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCgggg                                                              >  4:33325/1‑147 (MQ=255)                                                       aCTGCTCGGCGGTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCt                                                        >  5:155794/1‑147 (MQ=255)                                                            tCGGCGGTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAAc                                                   <  3:66521/147‑1 (MQ=255)                                                            tCGGAGGTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTGCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGaa                                                    <  4:72587/146‑1 (MQ=255)                                                                 ggTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTAt                                               >  1:197096/1‑146 (MQ=255)                                                                               tCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGAt                                >  3:35725/1‑147 (MQ=255)                                                                                  tGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGCCACCTGCCCGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCCGCGTGGCCGGATCGAg                            >  7:76256/1‑148 (MQ=255)                                                                                   gCCGATGAAGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGCAACTTATTCAGCTTGGCCGGATCGAg                            >  8:33094/1‑147 (MQ=255)                                                                                    ccGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAg                            <  4:35725/146‑1 (MQ=255)                                                                                     cGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGcc                          <  4:124460/147‑1 (MQ=255)                                                                                              tggCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCa                 <  6:155794/147‑1 (MQ=255)                                                                                               ggCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATg               <  8:113727/148‑1 (MQ=255)                                                                                                 cGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGc             <  4:109858/148‑1 (MQ=255)                                                                                                 cGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGc             <  6:10610/148‑1 (MQ=255)                                                                                                    tCAGCTGGGTGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGATGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTa          <  4:131542/148‑1 (MQ=255)                                                                                                             tGCCCGGCAGGGCTGGCAGGTCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCAc  >  8:95528/1‑147 (MQ=255)                                                                                                                                 |                                                                                                                               CGCCCAGGCGCACCTGTGAACCGTCTTTGTTGACCTTGAGCAGGATACTCTCGAACTGCTCGGCGGTTTGCAGGCGGGTCTTGCCGATGATGGTGGCGTTCAGCTGGGTGCCCGGCAGGGCTGGCAGGCCGCCGAGCTGGCCGGAAGACACCTGCACGTTCTGTGCGGCCACAGCGGTCTTGACGTCGACCGGGGTCAGCTGGAACTTATTCAGCTTGGCCGGATCGAGCCAGATACGCATGGCGTACTGCGCAC  >  NC_002947/1580607‑1580861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑