Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,715,625	A→T	M286L (ATG→TTG)	PP_4173 →	two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,715,625	0	A	T	100.0%	109.5 / NA	33	M286L (ATG→TTG)	PP_4173	two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/19);  total (14/19)

CAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCATGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGCTCAAGGTCATCAACGACATCCTCGATTTTTCGCGCATCGAACGCGCTG  >  NC_002947/4715484‑4715757                                                                                                                                              |                                                                                                                                     cAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTtgctgc                                                                                                                                <  3:98353/148‑1 (MQ=255)             gAACCGAATCAGGCCATCGCCGATCTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCTTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGaaa                                                                                                                    <  8:162268/148‑1 (MQ=255)             gAACAGAAGCAGGCCATCGCCGAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGaaa                                                                                                                    <  7:47985/148‑1 (MQ=255)              aaCAGAAGCAGGCCATCGCCGAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAAc                                                                                                                   >  4:41005/1‑148 (MQ=255)               aCAGAAGCAGGCCATCGCCGAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAAc                                                                                                                   <  6:68895/147‑1 (MQ=255)                       aGGCCATCGCCGAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGAGTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGc                                                                                                           >  7:50301/1‑147 (MQ=255)                        ggCCATCGCCGAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGc                                                                                                           >  8:27716/1‑146 (MQ=255)                              cGCCGAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGt                                                                                                   <  7:34044/148‑1 (MQ=255)                                  gAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGa                                                                                                 >  5:162134/1‑146 (MQ=255)                                    aCTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGACACCACACCGCTGAGCAGTGAACAg                                                                                             >  3:45589/1‑148 (MQ=255)                                      tGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGc                                                                                           <  3:41005/148‑1 (MQ=255)                                      tGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGc                                                                                           >  4:139797/1‑148 (MQ=255)                                          ccAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGa                                                                                        <  2:50221/147‑1 (MQ=255)                                           cAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGAtt                                                                                      <  3:31714/148‑1 (MQ=255)                                            aGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCGGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGAtt                                                                                      <  7:86493/147‑1 (MQ=255)                                                 cgcgAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACgg                                                                                <  7:27716/148‑1 (MQ=255)                                                   cgAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCa                                                                              <  5:115299/148‑1 (MQ=255)                                                     aggaggCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGt                                                                            <  3:6505/148‑1 (MQ=255)                                                                     cAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCAcc                                                            <  3:76382/148‑1 (MQ=255)                                                                      aaCCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCg                                                           >  4:39384/1‑148 (MQ=255)                                                                              cAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTg                                                   >  1:190422/1‑148 (MQ=255)                                                                                aaTCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGc                                                  >  6:110942/1‑147 (MQ=255)                                                                                aaTCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGc                                                  >  8:27191/1‑147 (MQ=255)                                                                                   cGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGCTCaa                                              <  3:59726/148‑1 (MQ=255)                                                                                        ttCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGCTCAAGGtca                                         >  4:173575/1‑148 (MQ=255)                                                                                           cTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGCTCAAGGtcatc                                       <  3:139797/147‑1 (MQ=255)                                                                                            tGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGCTCAAGGTCATCaa                                     >  2:28343/1‑148 (MQ=255)                                                                                                     tgaGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGCTCAAGGTCATCAACGACATCCt                            <  7:90689/148‑1 (MQ=255)                                                                                                        gCCATGAGTTGCGCACCCCGATGACTGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTTGCCAGCGAGTCCACCGGGCATTTGCTCAAGGTCATCAACGACATCCTCGa                         <  7:167577/148‑1 (MQ=255)                                                                                                        gCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGCTCAAGGTCATCAACGACATCCTCGa                         <  2:190422/148‑1 (MQ=255)                                                                                                         ccATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGCTCAAGGTCATCAACGACATCCTCGAt                        >  1:22643/1‑148 (MQ=255)                                                                                                                        cccGATGAATGGCGTGCTGGGCTTGATGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGACAGCGAGTCCACCGGGCATTTGCTCAAGGTCATCAACTCCATCCTCGATTTTTAGCGAATCGaa         >  7:162023/1‑148 (MQ=255)                                                                                                                                aTGGCGTGCTGGGCTTGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGCTCAAGGTCATCAACGACATCCTCGATTTTTCGCGCATCGAACgcgctg  <  2:16401/147‑1 (MQ=255)                                                                                                                                              |                                                                                                                                     CAGGCCAGCGATGAACAGAAGCAGGCCATCGCCGAACTGATCCAGGCCCGCGAGGAGGCCGAACAGGCCAACCGTGCCAAATCGGATTTCCTGGCGATGATGAGCCATGAGTTGCGCACCCCGATGAATGGCGTGCTGGGCATGCTGCAACTGCTGGAAACCACACCGCTGAGCAGTGAACAGGCCGATTACACGGCAGTGGCCAGCGAGTCCACCGGGCATTTGCTCAAGGTCATCAACGACATCCTCGATTTTTCGCGCATCGAACGCGCTG  >  NC_002947/4715484‑4715757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑