Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,816 T→G intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8160TG97.8% 150.0 / NA 46intergenic (+53/+700)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (20/25);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACC  >  NC_002947/4740680‑4740953
                                                                                                                                        |                                                                                                                                         
aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGcc                                                                                                                                  <  5:188415/145‑1 (MQ=255)
aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                               <  1:7024/148‑1 (MQ=255)
    cGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                           <  8:44476/148‑1 (MQ=255)
      tACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc                                                                                                                         <  5:79289/148‑1 (MQ=255)
         aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCCTCTTCGCgg                                                                                                                     >  3:119604/1‑148 (MQ=255)
                 ccGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGcc                                                                                                              >  3:106873/1‑148 (MQ=255)
                       gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCCAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                        <  6:43722/148‑1 (MQ=255)
                       gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGaa                                                                                                        <  4:119604/147‑1 (MQ=38)
                       gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctc                                                                                                                            >  8:85060/1‑128 (MQ=255)
                       gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCGGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTGGCCCGCGaa                                                                                                        <  5:109342/148‑1 (MQ=255)
                        cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                           <  7:85060/128‑1 (MQ=255)
                             gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGCAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGCAGGCTGCCCTCGGGCAGCC‑TTTTGTATTTGTCCTGTGCCGGCCTCTTCGCGGCCGTGCCCGCGAAGAGGcc                                                                                                  <  6:72358/148‑1 (MQ=255)
                                gTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGTGGCCGGc                                                                                               <  4:106873/148‑1 (MQ=255)
                                   cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGACCTCGGGCAGCC‑TTTTTTATTTGTACTGTGCCGGCCTCTTAGCGGCCTTGACCGCGAAGAGGCCGGc                                                                                               >  8:45931/1‑145 (MQ=255)
                                    acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGTCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                                 <  4:159786/126‑1 (MQ=255)
                                    acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                                 <  8:85211/126‑1 (MQ=255)
                                      cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGac                                                                                         >  2:133085/1‑148 (MQ=255)
                                       ggCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTAGCGGCCGTGCCCCCGCAGAGGCCCGccccgcca                                                                                        >  2:85786/1‑141 (MQ=255)
                                              aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCCCGGCCTTGCCCCCGAAGAGGCCGGCCCAGACCCCCCaac                                                                                 >  4:98887/1‑147 (MQ=255)
                                              aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCATCGGGCAGCC‑TTTTTTATTTGTCATGTGCCGGCCGCTTCGCGGACTTGCCCGCGAAGAGGCCGGCACAGACACCACaaa                                                                                 >  4:39153/1‑148 (MQ=255)
                                                  gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCt                                                                             >  7:128226/1‑148 (MQ=255)
                                                    gcgACATCGTTGCCCTCAACGACCGAAAGTACGCTGCCTTAGCCCAACGCAAGACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCa                                                                           <  6:86546/148‑1 (MQ=255)
                                                      gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAgg                                                                         >  8:69889/1‑147 (MQ=255)
                                                          tCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGttttt                                                                     >  8:168932/1‑148 (MQ=255)
                                                                 ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcc                                                              >  8:136598/1‑148 (MQ=255)
                                                                          ccGCACGTACGCTGCTGACGCCTACCGCAAGATGGCGGCCCTCGGGCAGGCTTTTTTTTTTGTCCTGG‑GCCGGCCGCTTCGCGGCGTGGCCCGCTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc                                                     <  2:173431/148‑1 (MQ=255)
                                                                           cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTCGCGGCCGTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCa                                                    <  5:130475/148‑1 (MQ=255)
                                                                                     cTGCAGTACCCGACCGCACGACGGCGGCCCGCGGGCAGCC‑TTTTTTATTTGGCCTGGGCCGGCCTCTTCGCGGCCTGGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                          <  1:127899/148‑1 (MQ=255)
                                                                                      tGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                         <  7:136598/148‑1 (MQ=255)
                                                                                        cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                          >  4:74712/1‑145 (MQ=255)
                                                                                         aGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                         <  3:74712/145‑1 (MQ=255)
                                                                                                gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCACTTTTTATCCGCCCCCTCACGCAGCCCTTTCAGGGTATTGAAcc                               >  4:49775/1‑147 (MQ=255)
                                                                                                 aaCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACgg                              <  1:133085/148‑1 (MQ=255)
                                                                                                       agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGCGGCCGGGCCAGCCACCCCAAAACCGCAGGTTTTCTCCGCCCGCTCACGCAGCCCGGTCAGGGTATTGAACGgcgccta                        >  3:119967/1‑145 (MQ=255)
                                                                                                       agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                        <  7:168932/148‑1 (MQ=255)
                                                                                                       agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTATCCGCCCGCTCACGCAGCCCGTTCAGGGTATTGAAAGGCGCAt                         >  2:19392/1‑147 (MQ=255)
                                                                                                         aagGCTGCCCGGGGTCTGCC‑TTTTTTATTTGCCCTGTGCCGGCCGCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcca                      <  3:98887/148‑1 (MQ=255)
                                                                                                            gCTGCCCTCGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca                   <  8:15484/148‑1 (MQ=255)
                                                                                                                 ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc              <  4:37674/148‑1 (MQ=255)
                                                                                                                 ccTCGGGCAGCC‑TTTTTTATTTGACCTGTGCCGCCCTCTTCGCGGCCTTGCCCGCTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc              <  5:81412/148‑1 (MQ=255)
                                                                                                                      ggCAGCC‑TTTTTTATTTGTCCTTGGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt         <  2:152614/148‑1 (MQ=255)
                                                                                                                        cAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAACCCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCGTTCAGGGTATTGAACGGCGCATCCACCCCGCACTTGTTgg       >  3:141089/1‑148 (MQ=255)
                                                                                                                           gcattttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcc     <  8:128226/145‑1 (MQ=255)
                                                                                                                            ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCCCAAAACCTCAGTTTTTCTACGCCCGCTCACGCAGCCCTTTAAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcca    >  2:141333/3‑148 (MQ=255)
                                                                                                                              ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGGAGAAGCCGGCACAGACACCACAACACCTCAGTTTTTCTCCGCCCGCTCACGCCGCCCGATCAGGGTATTGACCGGCGCAGGCACCACGCACTTGTTGGcca    >  6:120910/1‑146 (MQ=255)
                                                                                                                              ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCCCGCAGCCCTTTCAGGGTATTGAACGGGGGATCCCCCACGAACTTGTTGGccccc  >  7:38032/1‑148 (MQ=255)
                                                                                                                                        |                                                                                                                                         
AGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACC  >  NC_002947/4740680‑4740953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑