Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,948,189	G→C	D95E (GAC→GAG)	rho ←	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,948,189	0	G	C	100.0%	94.2 / NA	28	D95E (GAC→GAG)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (12/16);  total (12/16)

TTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGAT  >  NC_002947/5948049‑5948329                                                                                                                                             |                                                                                                                                             ttCGGGAACAGCGGCGTCAGGTGTGCGACCAGGATCTTGTTCTTCGCGTTTTCCGGGCGGGCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGGCTCGCCg                                                                                                                                        <  2:65515/147‑1 (MQ=255)          aGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGt                                                                                                                              <  1:191021/148‑1 (MQ=255)                 tCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGaa                                                                                                                          >  1:86623/1‑145 (MQ=255)                  cAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGaaa                                                                                                                         <  2:86623/145‑1 (MQ=255)                    ggTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAAcggcg                                                                                                                    <  7:2184/148‑1 (MQ=255)                    ggTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTAGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAAcggc                                                                                                                     <  2:190963/147‑1 (MQ=255)                                 gATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGc                                                                                                       <  2:206353/148‑1 (MQ=255)                                           ttcGCGTTTTGGGGGCGGTCGAAGTTGATGGTATCGAAATACAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGACGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGa                                                                                             >  4:141797/1‑148 (MQ=255)                                              tcgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGat                                                                                            <  2:91433/145‑1 (MQ=255)                                              gcgTTTTCCGGGCGGTCGAAGTTGCTGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGa                                                                                             <  7:159595/145‑1 (MQ=255)                                                             tCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGCCGGATCTTGCCGACGATGGTCTCGACGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCa                                                                           >  8:10751/1‑148 (MQ=255)                                                                          tctCGACCTTCCGCAGGGCGAAGTAACGCGCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCGCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATc                                                              <  4:106610/146‑1 (MQ=255)                                                                          tATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCAt                                                               <  2:113547/147‑1 (MQ=255)                                                                            tCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAg                                                            >  3:124859/1‑148 (MQ=255)                                                                              gACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGc                                                           >  3:50441/1‑147 (MQ=255)                                                                                  ttCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAAc                                                      <  4:3037/148‑1 (MQ=255)                                                                                      gcagGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCa                                                   <  3:22214/147‑1 (MQ=255)                                                                                            cGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGgaaacc                                            <  6:119896/148‑1 (MQ=255)                                                                                                     gCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATc                                   >  1:94229/1‑148 (MQ=255)                                                                                                       tacccTTCCTTCGGTGGGCGGATCTTGGCGACGATGGTCTCGCGGGTACGCAGGTTGAAACGGCGGGTCTGGGTGGGCGAGCGATAGATATGGTCTGGGCCTGCCAGGTAGGGCGCCTCAGCCGAACGCAGGAAACGGAGACCATCCt                                 >  4:21038/3‑148 (MQ=255)                                                                                                          ccttccttCGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTg                                <  8:44770/146‑1 (MQ=255)                                                                                                            ttccttcGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGgaga                            <  7:118776/148‑1 (MQ=255)                                                                                                            ttccttcGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGgaga                            >  7:93513/1‑148 (MQ=255)                                                                                                             tccttcGGTGGGCGGATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGaa                           >  6:86042/1‑148 (MQ=255)                                                                                                                ttcGGTGGGCGGATCTTGCCGACGATGGTCTTGCCGGTACGCAGGTTGAAACGGAGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCAGGCCAGGTAGGACGCATCAGCAGACCGCAGGACACCGAAACCATCCTGGAGAAtct                        >  4:21594/1‑148 (MQ=255)                                                                                                                          ggATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAACCCGAAACCATCCTGGAGAATCTCCAGCACGcc              >  8:61103/1‑148 (MQ=255)                                                                                                                           gATCTTGCCGACGATGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCg             >  5:82545/1‑148 (MQ=255)                                                                                                                                      cgaTGGTCTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGAt  <  4:50441/148‑1 (MQ=255)                                                                                                                                             |                                                                                                                                             TTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGAT  >  NC_002947/5948049‑5948329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑