| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 96.5% | 90.7 / ‑3.3 | 29 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (15/13); total (16/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCA > NC_002947/1932496‑1932774 | gcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑cccgcg < 2:46214/149‑5 (MQ=255) tCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAg < 3:250483/149‑1 (MQ=255) ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTATGAGCGGGCTTGCCC‑GCGAAggg > 6:293744/1‑149 (MQ=255) gagGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGc > 4:271451/1‑147 (MQ=255) aGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAg > 3:47054/1‑131 (MQ=255) cACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAt > 6:105250/1‑149 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑c < 6:313611/149‑1 (MQ=255) aaTAGCCTTACACGCTGGGGCGGATTTGCGGCCCTTCGGGGGCAAGCCCGCTCCTACAACGTTTATGCGTCCCCTGTCGGCGGGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCa < 8:58576/149‑1 (MQ=255) aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa > 6:138134/1‑128 (MQ=255) aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa < 5:138134/128‑1 (MQ=255) aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑A‑CGGCAGGGTGATGt > 2:232905/1‑148 (MQ=255) aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCACCCAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCACGGTGATGtt > 2:228316/1‑149 (MQ=38) cTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATc < 3:271451/149‑1 (MQ=255) gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTg < 5:105250/149‑1 (MQ=255) gggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGt > 5:223227/1‑149 (MQ=255) gCAAGCCCGCTCCTACAAATCATATTCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAATGGCTGCAATGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑CNCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtt < 1:232905/149‑1 (MQ=255) gCAAGCCCGCTCCTACAAAGGTTATGCGTCCCCTGTTGGTGGGGGTTTGCCC‑GCGAAGGGCTGCAAGGGAGCCCCAAATTGTGGACTACGCGGATCAGCGATCC‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtt < 1:228316/149‑1 (MQ=255) gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt > 8:1262/1‑149 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc > 6:102563/1‑149 (MQ=255) aGCCCTGTCCTAAAATGTTTATGCGTCCCTTGTTGTAGCGGTCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc < 5:293744/149‑1 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 3:19026/1‑149 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata > 2:18524/1‑149 (MQ=255) atatGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca > 1:312316/1‑149 (MQ=255) tGCGTCCCCTGTAGGAGCTGGATTGCCC‑GCGAAGGGCTGCAAGTCAGCCCCAAATTGTCGACTACGCTGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 5:15146/148‑1 (MQ=255) gCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTgg > 5:111372/1‑121 (MQ=255) gCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTgg < 6:111372/121‑1 (MQ=255) gCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATAGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt > 3:275373/1‑149 (MQ=255) cccTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCg < 5:102563/149‑1 (MQ=255) gTAGGAGCGGGCTTGCCC‑GCGAAGGGCCGCAAAGCGGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATg < 1:171904/148‑1 (MQ=39) gAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca > 8:275556/1‑148 (MQ=255) | GCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAT‑C‑CGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCA > NC_002947/1932496‑1932774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |