Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	336,125	+T	coding (210/237 nt)	PP_0278 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	336,124	1	.	T	100.0%	137.0 / NA	41	D70V (GAT→GTT)	PP_0278	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (20/21);  total (20/21)

GTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335976‑336263                                                                                                                                                       |                                                                                                                                             gTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGa                                                                                                                                               <  2:152403/149‑1 (MQ=255)    aaCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑tt                                                                                                                                            <  7:239490/148‑2 (MQ=255)       ccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacct                                                                                                                                        >  5:239002/1‑144 (MQ=255)         aTCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGTGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCtgt                                                                                                                                       <  3:72646/149‑1 (MQ=255)         aTCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCtgt                                                                                                                                       >  1:84808/1‑149 (MQ=255)             cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGa                                                                                                                                   >  6:86345/1‑149 (MQ=255)             cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGa                                                                                                                                   >  7:181277/1‑149 (MQ=255)                      cTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATgg                                                                                                                              <  2:149372/145‑1 (MQ=255)                      cTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑aa                                                                                                                         <  2:84808/149‑1 (MQ=255)                         cgcgTAGAACCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCAAGGTCCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAgg                                                                                                                       <  1:97584/149‑1 (MQ=255)                                  cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGaa                                                                                                             >  6:203688/1‑149 (MQ=255)                                    cGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGgc                                                                                                                      >  2:250445/1‑138 (MQ=255)                                    cGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGgc                                                                                                                      <  1:250445/138‑1 (MQ=255)                                       cGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGata                                                                                                        <  6:239002/149‑1 (MQ=255)                                          ccccGGCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGGGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCg                                                                                                     <  6:169841/149‑1 (MQ=255)                                                            cGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAgg                                                                                   >  8:119820/1‑149 (MQ=255)                                                             gAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAAGGCGCTGATGAACGATATCgag                                                                                                   <  7:46722/133‑1 (MQ=255)                                                             gAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCgag                                                                                                   >  8:46722/1‑132 (MQ=255)                                                             gAGCATGTCTCGATACTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgc                                                                                  >  2:329198/1‑149 (MQ=255)                                                              aGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgcg                                                                                 <  2:45589/149‑1 (MQ=255)                                                               gCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgcg                                                                                 >  5:195582/1‑148 (MQ=255)                                                                      tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAgc                                                                          >  7:93526/1‑147 (MQ=255)                                                                       cGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCa                                                                        >  6:74710/1‑149 (MQ=255)                                                                        gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAgg                                                                          <  5:86345/146‑1 (MQ=255)                                                                        gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAt                                                                       >  5:130591/1‑149 (MQ=255)                                                                        gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAt                                                                       <  7:119820/149‑1 (MQ=255)                                                                          tCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTg                                                                     <  1:329198/149‑1 (MQ=255)                                                                             tGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcg                                                                  >  7:219883/1‑149 (MQ=255)                                                                                  ggCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTc                                                             <  8:219883/149‑1 (MQ=255)                                                                                       cATCAAGCACCTGGTGCGCGAGG‑GCCATATGGAGGACGACCATAAATTCCTGGGGGCAGGCGATTACCTGTGCGACATGGCC‑CAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAttt                                                         <  5:261507/148‑1 (MQ=255)                                                                                         tCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                      <  6:130591/149‑1 (MQ=255)                                                                                                ccTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATc                                                                                                >  6:239788/1‑100 (MQ=255)                                                                                                ccTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATc                                                                                                <  5:239788/100‑1 (MQ=255)                                                                                                 cTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGc                                              <  6:195582/149‑1 (MQ=255)                                                                                                     tGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTg                                          >  7:39828/1‑149 (MQ=255)                                                                                                       cgcgAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGc                                         <  8:181277/148‑1 (MQ=255)                                                                                                        gcgAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                       >  7:171229/1‑133 (MQ=255)                                                                                                        gcgAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                       <  2:318751/133‑1 (MQ=255)                                                                                                        gcgAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                       <  8:171229/133‑1 (MQ=255)                                                                                                        gcgAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                       >  1:318751/1‑133 (MQ=255)                                                                                                                                     aTTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGc           >  2:120198/1‑149 (MQ=255)                                                                                                                                     aTTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCATGCGTAGCGCAGGGGCTGCAAAGCCCCACCGGc           >  3:43324/1‑149 (MQ=255)                                                                                                                                         cTGGGGGCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGAtt       >  6:245882/1‑149 (MQ=255)                                                                                                                                              ggCAGCCGATTACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCaga  <  5:245882/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                             GTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGG‑GCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCC‑AAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335976‑336263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑