| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,988,889 | 0 | C | A | 34.0% | 59.8 / 39.3 | 50 | intergenic (+183/‑363) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base C (14/19); new base A (8/9); total (22/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCAC > NC_002947/5988747‑5989031 | gaTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGca < 3:149566/149‑1 (MQ=255) ttGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCTCGCCTCGATATCGCGTCTTACAAACAAGGCTGTCGCGCG‑CGCCTGGcaca < 3:106962/149‑1 (MQ=255) gccgccCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACaggagg > 2:231959/1‑148 (MQ=255) gcccgcGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGAtt > 5:36871/1‑149 (MQ=255) ccgcGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCGAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTAGAAAACGCGGCGTACAAACAAGGCGGTCGCGCG‑AGCCTGGCACAGGATGGATTgg > 4:166742/1‑149 (MQ=25) cgcgcgGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGc < 6:36871/149‑1 (MQ=255) gcgcgGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGcc < 7:245334/149‑1 (MQ=255) ggcgCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCG‑CCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGccgaaa > 7:252600/1‑145 (MQ=14) gggTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGGGCCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAg > 1:258431/1‑149 (MQ=14) cccGCTCCTACGCTTGTATCGGGCCAGTTATACCGGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGACTGGCCAGAAACAAACGTAGGAGCggg < 3:166742/149‑1 (MQ=21) ccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGt > 2:271792/1‑149 (MQ=255) ccGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCG‑CCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGtt > 3:72919/1‑149 (MQ=11) cGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCCCCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGt < 1:231959/149‑1 (MQ=255) gtttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcg < 8:114892‑M2/148‑7 (MQ=255) gtttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcg < 6:324061‑M2/148‑7 (MQ=255) gtttgtttCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCG‑CCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCcgcg > 5:125759/1‑147 (MQ=11) cGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTg < 8:280606/106‑1 (MQ=255) cGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTg > 7:280606/1‑106 (MQ=255) ggCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTATAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgc < 2:134693‑M2/149‑18 (MQ=255) gTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATGGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcg > 1:271792‑M2/1‑127 (MQ=255) aTTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcgg > 3:143375‑M2/1‑124 (MQ=255) aTTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcgg < 4:143375‑M2/147‑24 (MQ=255) tgtgGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcga < 6:294014‑M2/149‑31 (MQ=255) tgtgGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcga < 5:314086‑M2/149‑31 (MQ=255) gAGCC‑CCCTGGCGCACGACTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCTCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGTGCGCCCa > 2:181712/1‑148 (MQ=255) ggggCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccgg < 4:247735‑M2/149‑57 (MQ=255) gctcACTCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGTCCAGAAACAAACGTAGGAGCTGTTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATc < 1:181712/144‑1 (MQ=21) gcgcACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATc < 6:314086/147‑1 (MQ=255) ggcaTGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggc < 4:239882‑M2/147‑58 (MQ=255) cTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCAcc > 7:175383/1‑149 (MQ=255) cTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacaac < 3:199931‑M2/148‑65 (MQ=255) gcgTCGTACAAACAAGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattc > 3:247735‑M2/1‑75 (MQ=255) tACAAACAAGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgcc < 6:44151‑M2/149‑81 (MQ=255) acaaacaaGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccg < 3:164434‑M2/149‑82 (MQ=255) acaaacaaGCCGGTCGCGCG‑CGCCTGGCACAGGACGGATTGGCCAGAAACAAACGTAGGAGCGGGTATACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCAGc > 6:188756/1‑148 (MQ=255) caaacaaGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCaa < 5:294014/149‑1 (MQ=255) aaacaaGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaa < 2:62396‑M2/136‑71 (MQ=255) aaacaaGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctccgatctcacaggcgctgaaaatcctccggcaaacacctcaaaa > 1:62396‑M2/1‑66 (MQ=255) aacaaGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGc < 7:172954/122‑1 (MQ=255) aacaaGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGc > 8:172954/1‑122 (MQ=255) acaaGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAttt > 3:68902/1‑149 (MQ=255) acaaGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAttt > 1:302251/1‑149 (MQ=255) acaaGGCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcacgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtcca < 2:23486‑M2/149‑86 (MQ=255) aaGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTcc < 5:324061/149‑1 (MQ=255) ggCGGTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactc < 3:11978‑M2/148‑89 (MQ=255) cGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCaaaaa > 2:134644/1‑108 (MQ=255) cGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCaaaaa < 1:134644/108‑1 (MQ=255) ggTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgct > 8:55889‑M2/1‑57 (MQ=255) gTCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaat > 3:239882‑M2/1‑56 (MQ=255) tCGCGCG‑CGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaatt < 2:270058‑M2/149‑95 (MQ=255) gcgcg‑cgaCTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattcc > 5:44151‑M2/1‑53 (MQ=255) | GATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCC‑CCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCG‑CGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCAC > NC_002947/5988747‑5989031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |