| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,464,196 | 0 | G | A | 29.0% | 38.4 / 17.7 | 32 | T474T (ACC→ACT) | algX | alginate biosynthesis protein AlgX |
| Reads supporting (aligned to +/- strand): ref base G (13/9); new base A (4/5); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.16e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGT > NC_002947/1464059‑1464344 | ttCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAgg > 3:98961/1‑149 (MQ=255) tCGATCCCCTGCTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCAGACCGCGCAgg < 1:325220/148‑1 (MQ=255) gATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCa < 4:98961/148‑1 (MQ=255) gATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCAGACCGCGCAGGCag < 6:221071/149‑2 (MQ=255) gATCCCCTGGTAGAAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCAGACCGCGCAGGCag < 8:59678/149‑2 (MQ=255) ccTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCGGCCCCGGGTGCGCCGGCCCcgcg > 5:52283/1‑148 (MQ=255) tGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAg > 2:1341/1‑149 (MQ=255) tGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAg > 3:139316/1‑149 (MQ=255) tGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAg > 3:144176/1‑149 (MQ=255) agtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttgt > 2:85708/1‑149 (MQ=255) taccccTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCGGGAAATTCGTTTGAAAACGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCAGACCGCGCAGGCa < 8:208375/132‑1 (MQ=255) taACCCTGCGGCGGTACCAGCGCGGCGGGCGCGGCACCACCCAACAACGCCAACGCGAGCAGGGCGGGGCTGGACATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCAGACCGCGCAGGCa < 8:208374/135‑1 (MQ=255) tGCGGCGGTACCAGCGCTGCGTGCGCGTCACCACCAAACAAGGCAAACGCGAGCTGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCCTCCTCACTGGC‑CTGCCTGCGGGGTCTGCGCTGGCACGGCGAAGTTGTTGCGttt < 1:85708/149‑1 (MQ=255) tGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCa > 4:244220/1‑125 (MQ=255) tGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCa < 3:244220/125‑1 (MQ=255) cggcggTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTg < 5:285914/147‑1 (MQ=255) cggcggTACCAGCGCGGCGGGCGCGGCACCACCAACCACCGCCACCGCGAGCCGGGCGGGGCGGGAAATTCGGTTGAAACGGGTCCTGGGG‑CGCCTCCCTGGC‑CTGCCGGCGCGGTCGGCGCAGGCACGGCGAAGTTGTTGCGTTTg < 5:280638/147‑1 (MQ=255) aaaCAAGGCCACCGCGAGCCGGGCGGGGCTGGAAATGCGTTTTAAACGGGTCATGGGG‑CGCCTCACTGCC‑CGGCCTGCGCGGTCTGCGCAGCCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTc < 1:131703/149‑1 (MQ=255) gAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTTTTGCGTTTTCAGAGCTTGGCTTCGAACTTCTGGGTGCCGCTTTCCGGCCCCTGTTCCTc > 6:34613/1‑149 (MQ=255) cGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAAcgc > 7:72720/1‑147 (MQ=255) gggCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGc < 1:1341/149‑1 (MQ=255) aaaGGGTCATGGGT‑CGCCTCACTGGCGCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAg > 1:4247/1‑149 (MQ=255) tCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACATCGAACGCGAGCATGGGCTGGCTGGCCAAGTGCTc > 6:226891/1‑147 (MQ=255) aTGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCt > 8:26846/1‑149 (MQ=255) tGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTgg > 7:176840/1‑147 (MQ=255) cgtgcctgcGCAGACCGCGCAGGCACGGCGAAGTTGTTGGGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGAc > 4:44196/3‑149 (MQ=255) cgtgcctgcGCAGACCGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGAc > 8:216549/3‑149 (MQ=255) ctgcctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGAc > 5:111404/1‑148 (MQ=255) ctgcctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACg < 6:111404/149‑1 (MQ=255) gcctgcGCAGACCGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCg > 6:168330/1‑93 (MQ=255) gcctgcGCAGACCGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACg > 4:90431/1‑147 (MQ=255) tgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCg < 8:72720/149‑1 (MQ=255) ggTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGGGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGt > 6:104689/1‑149 (MQ=255) | TTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGT‑CGCCTCACTGGC‑CTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGT > NC_002947/1464059‑1464344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |