Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 3,167,544 C→T S227S (AGC→AGT)  PP_2781 → beta‑ketoacyl synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,167,5440CT100.0% 107.0 / NA 34S227S (AGC→AGT) PP_2781beta‑ketoacyl synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/20);  total (14/20)

CAAGGTGTCGCCGGCAACCTGGCAGCCTTGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGCGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAGGCGTGCCTCGCCGACATGACGGCCCA  >  NC_002947/3167398‑3167687
                                                                                                                                                  |                                                                                                                                               
cAAGGTGTCGCCGGCAACCTGGCAGCCTTGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAgtgc                                                                                                                                               >  4:177514/1‑149 (MQ=255)
 aaGGTGTCGCCGGCAACCTGGCAGCCTTGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAgtgc                                                                                                                                               >  4:195032/1‑148 (MQ=255)
   ggTGTCGCCGGCAACCTGGCAGCCTTGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCaa                                                                                                                                            <  1:200869/149‑1 (MQ=255)
        cGCCGGCAACCTGGCAGCCTTGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCt                                                                                                                                       >  4:208689/1‑149 (MQ=255)
          ccGGCAACCTGGCAGCCTTGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCg                                                                                                                                     <  3:195032/149‑1 (MQ=255)
          ccGGCAACCTGGCAGCCTTGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCg                                                                                                                                     <  5:112336/149‑1 (MQ=255)
            ggCAACCTGGCAGCCTTGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGaa                                                                                                                                   <  4:134411/149‑1 (MQ=255)
             gCAACCTGGCAGCCTTGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAac                                                                                                                                  <  6:39011/149‑1 (MQ=255)
             gCAACCTGGCAGCCTTGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAac                                                                                                                                  <  6:271781/149‑1 (MQ=255)
                           ttGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGtt                                                                                                                    >  8:235849/1‑149 (MQ=255)
                             gCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGtt                                                                                                                    <  1:122853/147‑1 (MQ=255)
                                       aCGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCgggg                                                                                                        >  1:219362/1‑149 (MQ=255)
                                                    aGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCAtt                                                                                           <  8:96093/149‑1 (MQ=255)
                                                     ggCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTg                                                                                          >  8:14087/1‑149 (MQ=255)
                                                       cTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGcc                                                                                        >  8:240466/1‑149 (MQ=255)
                                                           ccGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCgggg                                                                                    <  4:32816/149‑1 (MQ=255)
                                                            cGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGa                                                                                   <  7:58914/149‑1 (MQ=255)
                                                                   aCGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGcgcg                                                                            >  6:274429/1‑149 (MQ=255)
                                                                   aCGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGcgcg                                                                            >  6:269960/1‑149 (MQ=255)
                                                                      cgGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCa                                                                         <  3:177514/149‑1 (MQ=255)
                                                                         cAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCg                                                                      >  1:170225/1‑149 (MQ=255)
                                                                                       gcgGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCg                                                        <  5:274429/149‑1 (MQ=255)
                                                                                       gcgGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGGCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCg                                                        <  5:269960/149‑1 (MQ=255)
                                                                                              gCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGaa                                                 <  7:235849/149‑1 (MQ=255)
                                                                                               cGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGgc                                                      >  8:81170/1‑143 (MQ=255)
                                                                                               cGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGGCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGgc                                                      <  7:81170/143‑1 (MQ=255)
                                                                                                                     tCCGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAg                            <  7:14087/147‑1 (MQ=255)
                                                                                                                       cGGGTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAGGCGt                        <  4:126114/149‑1 (MQ=255)
                                                                                                                         ggTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAGGCGTGc                      <  2:219362/149‑1 (MQ=255)
                                                                                                                          gTTGGTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAGGCGTGcc                     <  7:240466/149‑1 (MQ=255)
                                                                                                                              gTCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAGGCGTGCCTCGc                 >  2:161029/1‑149 (MQ=255)
                                                                                                                               tCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAGGCGTGCCTCGcc                >  6:130766/1‑149 (MQ=255)
                                                                                                                               tCCAGGCGGGTGTCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAGGCGTGCCTCGcc                <  3:208689/149‑1 (MQ=255)
                                                                                                                                          gtCATCAGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAGGCGTGCCTCGCCGACATGACGGc     >  2:48994/1‑149 (MQ=255)
                                                                                                                                           tcatcaGTGACAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGGGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAGGCGTGCATCGCCGACATGACGGcc    >  5:247680/1‑149 (MQ=255)
                                                                                                                                             atcaGTGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAGGCGTGCCTCGCCGACATGACGGCCCa  >  4:189638/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CAAGGTGTCGCCGGCAACCTGGCAGCCTTGCGTGAGGCTACGGCCGCTTTGCAGGCTGGCCGAATTGACGCGGCAATCGTTGTCGGCGCGGGCAGCGAGCTGGATGCCCTGGCGCTGTCCGGGTTGGTCCAGGCGGGTGTCATCAGCGCCAACGGCTCGAACACGCTGCTGCCGTTCGACCTTCGGGGCACCGGCGGCATTGCCGGGGAGGGCGCGGCAGCGTTGGTGCTGCGCCGGCGCGAAGACCTGTCGGCCGGTCCTCAGGCGTGCCTCGCCGACATGACGGCCCA  >  NC_002947/3167398‑3167687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑