BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	111.5 / NA	34	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/16); total (18/16)

CCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAAC  >  NC_002947/499062‑499344

ccATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGc                                                                                                                                        >  1:271911/1‑149 (MQ=255)

     aGCACCCACAATGCACGCCTGTTGCATAAGTTGAAATTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCcgc                                                                                                                                    <  2:268895/148‑1 (MQ=255)

     aGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCcgc                                                                                                                                    <  2:185916/148‑1 (MQ=255)

          ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCcgcgcaagg                                                                                                                              >  3:217079/1‑144 (MQ=255)

          ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACgg                                                                                                                              <  2:271911/149‑1 (MQ=255)

              aaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGggcct                                                                                                                          >  6:232188/1‑149 (MQ=255)

               cggcagcccTGATGAAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGGACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGggcctg                                                                                                                         <  6:229809/142‑1 (MQ=255)

                       cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAAGGCCCCCAAACGgccgtggcggggg                                                                                                                  >  1:123604/1‑144 (MQ=255)

                       cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCctgct                                                                                                                  >  6:265910/1‑148 (MQ=255)

                         gATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCctgct                                                                                                                  >  6:152297/1‑146 (MQ=255)

                         gATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCctgct                                                                                                                  <  5:152297/146‑1 (MQ=255)

                           tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGAtc                                                                                                              <  2:65703/148‑1 (MQ=255)

                             cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGAtctc                                                                                                            >  5:130303/1‑148 (MQ=255)

                                 aGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATgg                                                                                                       <  4:69407/149‑1 (MQ=255)

                                  gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGAtc                                                                                                              <  5:90055/141‑1 (MQ=255)

                                  gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGAtc                                                                                                              >  6:90055/1‑141 (MQ=255)

                                       aaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGc                                                                                                                                        >  2:160742/1‑110 (MQ=255)

                                            aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGcc                                                                                            >  1:189394/1‑149 (MQ=255)

                                             aCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCct                                                                                           <  2:189394/149‑1 (MQ=255)

                                                 gCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATTCCCGCGAACGGGCCTGGCCTGCTGATCTCTATTGCTGAGCCGGCctct                                                                                         >  3:83341/1‑147 (MQ=255)

                                                     gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                   <  5:265910/149‑1 (MQ=255)

                                                         aGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCAt                                                                               <  5:232188/149‑1 (MQ=255)

                                                                    tACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCAGGCCGGCGGCACTCGATGGCTGAGCCGGCCTCTTAGCCGGCCTGcccccgacc                                                                     >  7:42623/1‑142 (MQ=255)

                                                                       gCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGggcc                                                                                                                           >  2:255050/1‑91 (MQ=255)

                                                                       gCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGggcc                                                                                                                           <  1:255050/91‑1 (MQ=255)

                                                                               gCACACGCTTATCTTATTGCGAAATACGGGGGCCCGAAACTTGCCGACGCCTATGTGGGTGCGGGC‑ATGCCCGCTAACGGGCCTGGCCTGCTGATCTATATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGt                                                          <  4:83341/148‑1 (MQ=37)

                                                                                 acaAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCTGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGt                                                       >  2:48903/1‑149 (MQ=255)

                                                                                             gatTGCGACATACGGGTGCCCGAAACTTACCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGGCTGCGGATGTCTATGGCTGAGCCGGCCTCTTAGCGGGCCTGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCCAgg                                           >  5:82476/1‑149 (MQ=255)

                                                                                                     cATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCACCCACAGGTTTAGTGGTTGGGTAGCAAGGGCATTcc                                    >  2:205315/1‑148 (MQ=39)

                                                                                                              gCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGTCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCa                          >  2:267650/1‑149 (MQ=255)

                                                                                                                    taCTTCCCTACGCCTTTGGGGGAGCGGGC‑ATGCCCGCGAACGGGCCGGGCCGGCTGATCTCTATGGCTGAGCGGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTg                    <  2:61845/148‑1 (MQ=38)

                                                                                                                             aaGCCTATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGAt           <  1:205315/149‑1 (MQ=255)

                                                                                                                                  tATGTGGGAGCGGGC‑ATGCCCGCGAACGGGCCTGGCCTGCGGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCCTGCCCGCTCCCACAGGGTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGGAGGGGGATcccga      >  5:99802/1‑147 (MQ=255)

                                                                                                                                      tgGGAGCGGGC‑ATGCCTGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTTCAATCCCGCCATCCTTGTAGGGATCGCCCTacaag  <  1:48903/149‑2 (MQ=38)

CCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGC‑ATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAAC  >  NC_002947/499062‑499344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`