| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,508 | +C | intergenic (+85/‑79) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,506 | 1 | . | C | 93.8% | 88.2 / 0.6 | 32 | intergenic (+83/‑81) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (12/18); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCC‑GGTG‑‑T‑T‑T‑‑CGGG‑CA‑CG‑CCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGC > NC_002947/1499362‑1499648 | cTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑cg‑ccc < 2:128283/149‑2 (MQ=37) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑Tcc > 5:364679/1‑149 (MQ=25) cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑Tccc < 7:221284/149‑1 (MQ=25) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCcac < 1:114419/149‑1 (MQ=25) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGTGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGa > 7:133774/1‑149 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTCCCGCGAAACACCCGCCCCCGTGCAAGGCACCCGCTATGCC‑CGTG‑‑T‑TAG‑‑CGGG‑CACCC‑CCGC‑TCCCCCAGACAGAATGCCATTAAGCg > 6:221192/1‑149 (MQ=255) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGTGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGc < 8:133774/148‑1 (MQ=21) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATc > 1:297915/1‑149 (MQ=21) cgCCTTGGCAGTCGAGCAGGTCTGAGGGTGCCATACGGTGGGAGCGGGGGTGGCCGCGAAACAGGCGCCGGGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 5:221192/148‑1 (MQ=18) aGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 2:297915/149‑1 (MQ=21) aGCCTTGGCACCACAGCAGGACTGAGGCGGCCAAACGGTGGGCGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 3:294754/149‑1 (MQ=21) ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa > 7:2431/1‑148 (MQ=21) ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCTTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa < 6:364679/148‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 8:111455/147‑1 (MQ=21) gCTGCCAAACGGTGGGAGCGGGCCTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCCGGTTTGGC‑GGAGGTT‑T‑TACCGGG‑CA‑CC‑CCCCAACCCACACAATGATTGGCATTAAGCGGGCCTATAGCTACAGTGACGGCCCGttttttt > 1:134977/1‑148 (MQ=255) tGCCAAACGGTGGGAGCGGGCGTGGCCGCGCAACACGCGCCCCGGTGGATTGCACCCGCGTTTCC‑GGTG‑‑T‑TAG‑‑CGGG‑GA‑CGCCCGC‑GCCCACAGACAGATTGGCAATTAACGGGGCGATAGGGAACATGACGGCCCGGTTTtttttggc > 8:14115/1‑147 (MQ=255) gAGGGGGCGGGCCCGCTAAACAGGCCCCGGGGTGCATGGCACGGGCTTTGCG‑GTTG‑‑T‑T‑C‑‑GGGGCCA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 3:402084/149‑1 (MQ=255) cgCGGGCTTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 8:371006/148‑1 (MQ=25) aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTTAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:11476/149‑1 (MQ=25) cGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 7:254113/149‑1 (MQ=25) gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc < 8:22796/149‑1 (MQ=37) cccGCTAAACCGGCGCCGCGGTGCA‑GGGCACGGGCTTGCCGGGTG‑‑TGC‑G‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt < 5:40226/149‑1 (MQ=255) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 8:6996/1‑147 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 2:333454/1‑148 (MQ=37) aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 5:90910/1‑148 (MQ=37) aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 5:131438/1‑148 (MQ=37) aGGCGCCGCTGTGCTTGTCAACGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGCGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt < 7:384999/149‑1 (MQ=37) ggCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 7:6996/149‑1 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 5:389653/1‑149 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg < 8:2431/149‑1 (MQ=37) cgGTGCATGGCACCGGCTTTGCC‑GGTG‑‑T‑TCG‑‑CGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGGTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 3:227702/1‑145 (MQ=37) gcGGG‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcg > 4:149198/2‑148 (MQ=255) gg‑CA‑CGCCCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcggc < 3:188046/149‑1 (MQ=255) | CTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCC‑GGTG‑‑T‑T‑T‑‑CGGG‑CA‑CG‑CCGC‑TCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGC > NC_002947/1499362‑1499648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |