| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 3,845,705 | +G | intergenic (‑72/+26) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 96.4% | 177.8 / ‑0.1 | 55 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (34/19); total (35/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01 | |||||||||||
GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑C‑GGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATT > NC_002947/3845557‑3845834 | gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCAGACTTCATAGATTGTTATTGTT‑GTCATAGCGGTCTCTCTGTCTGAAGCGTTGCGACACACTAAGAGATGGATGACGGGCAGAAAAGCCGTGTTT‑CG‑C‑gg > 8:309826/1‑149 (MQ=255) gTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGAt < 4:265716/149‑1 (MQ=255) ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCa > 1:178937/1‑149 (MQ=255) gCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCAt > 3:35183/1‑149 (MQ=255) tGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCAACGt > 1:173134/1‑149 (MQ=255) gatggcTTGCAGAGCCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGt > 5:60829/6‑148 (MQ=255) aTCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTTTTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGAGTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCa < 1:176633/149‑1 (MQ=255) aCTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGc > 5:34294/1‑148 (MQ=255) cTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCa > 3:167181/1‑149 (MQ=255) gcctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGAt < 6:60829/147‑1 (MQ=255) tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGa > 1:308165/1‑148 (MQ=255) tGCAAGCCATCGCGCAGGGCGACTTCATTGATTGTTATTGTT‑GCCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAg > 3:189172/1‑149 (MQ=255) tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATc < 8:119449/147‑1 (MQ=255) gCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGCGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCAACGATGGATCAG‑c > 1:309443/1‑149 (MQ=255) aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAATCGTTGCGCCAGACTAATAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑ccc > 7:279482/1‑149 (MQ=255) cATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCgcgc > 6:321035/1‑149 (MQ=255) cATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCgcgc > 3:38422/1‑149 (MQ=255) cATCGCGCAGGCCGACTTCATAGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTAAGCCAAGATGGATCAG‑CCcgccc > 1:257832/1‑147 (MQ=255) tCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGccc > 3:231986/1‑149 (MQ=255) cgcAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATg < 4:10183/131‑1 (MQ=255) cgcAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATg > 3:10183/1‑131 (MQ=255) cgcAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCcgcg < 2:235368/143‑1 (MQ=255) cgcAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCcgcg > 1:235368/1‑143 (MQ=255) cgcAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGc > 5:102150/1‑149 (MQ=255) cgcAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGc > 4:343811/1‑149 (MQ=255) ccAGGCCGACTTCATTCATTGTTATGGTT‑GTCATCGCGGGCTCTCTGTCTGAAGCGTGGCGCCAGACTAAGAGCGGGAGGAGGGGCACAAACCACGTGTTG‑CG‑CGGGCTGATCCAGCGCCAGCCACGATGGATCAG‑CCCGCGCCCTGcc < 8:322370/148‑1 (MQ=255) gACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTAAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCgg > 7:50724/1‑149 (MQ=255) attCATTGATTTTTATTGTT‑GTCTTCGGGGTCTCTCTGTCCTACGCTTTGCCCATTATTAATCGGTGCTTGAGGGCCATAAACGACGTTTTT‑CG‑CGGGCTTACCCACCTTCAGCCACTTTGTATCTG‑CCCGCGCCCTGCCAGGCCGGTg < 8:398081/148‑1 (MQ=255) attgatttTTTTTGTT‑GTCATCGGGGTCTCTCTGTCTGATGCTTTGCGCCATACTAAGAGCTGGATGAGGGGCATAAAAGACTTGTTTGGG‑G‑GGCTTATCCATCTTCAGCAACTTTGTATCAG‑CCCGCGCCCTGCCAGGCCGGTGCAc < 6:102150/148‑1 (MQ=255) ttgattgTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACg > 8:189439/1‑148 (MQ=255) tttttgtttttgtt‑GTCATCGCGGTCTCTCTGTCTTAAGCGTTGCGCAAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTTATCCATCGTCAGCCACTATGTATCTG‑CCCGCGCCCTGCCAGGCCGGTGCAc < 2:308165/143‑1 (MQ=255) tgattgTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACg > 7:28840/1‑147 (MQ=255) attgttattgtt‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACg > 2:409763/1‑110 (MQ=255) attgttattgtt‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACg < 1:409763/110‑1 (MQ=255) ttattgtt‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAAc < 3:343811/148‑1 (MQ=255) ttattgtt‑GTCATCGCGGGCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaa < 1:52072/148‑1 (MQ=255) ttgtt‑gTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg < 7:189439/148‑1 (MQ=255) tt‑gtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtggt > 1:27807/1‑149 (MQ=255) tCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCaa < 5:321035/149‑1 (MQ=255) tCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGACCTGGATGACGGGCAGAAAAGACGTGTAT‑CGCC‑GGCTGAACCATCGTCAGCCACGATGGATCAG‑CCCCCGCCCAGCCAGGCCGGAGCACGCAGCAACACGTGGTc > 2:159086/1‑147 (MQ=255) gTCTCTCTGTCTGAAGAGTTGCGCCAGACTAAGAGCTGTATGACGGGCAGAAAACAGGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACTATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGgcgc < 8:390891/149‑1 (MQ=255) tctctcTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgcc > 1:69887/1‑149 (MQ=255) ttGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGc > 6:427377/1‑149 (MQ=255) tggcccaaGACTAAGAGCTGGATGACGGGCAGAAAAGACGTTTTG‑CG‑CGGGCTGATCCAGCGTCAGCCACTTTGTTTCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGc < 8:279482/142‑1 (MQ=255) cgcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgt < 2:69887/148‑1 (MQ=255) gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt > 4:113540/1‑149 (MQ=255) gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt > 5:358921/1‑149 (MQ=255) ccAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGtgtg > 7:368358/1‑149 (MQ=255) cAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCAACGTCAGCCACGATGGATAAG‑CCCGCGCCCATCCAGGCCGGTGCACGCAGCAACAGGTGGTAAAGCAGGCGCCGAGCAGACAGCCAGAGCGTGTGc > 7:150362/1‑149 (MQ=255) tccTAATAGCGGGTTGACGGGCATAAAAGACGGGTTT‑CG‑CGGGCGGAGCCATGGTCTGCCACGATGGTTCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAACCGACAGCGAGAGCGTGTGCCg < 2:309443/147‑1 (MQ=255) gagCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcgcg > 4:148777/1‑149 (MQ=255) ggATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCCGGCCGGTGCACGCAGCAACAGGTGGTACAGCAGGCGCCGAGCCGACCGCGAGGGCGTGTGCCGGTCGCGCATGc > 8:344337/1‑149 (MQ=255) gATGACGGGCAGAAAAGACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg > 8:255659/1‑147 (MQ=255) gAAAAGACGTGTTT‑CG‑CGGGCAGATACAACCTCAGCCCCCATGGGTAAG‑CCCCCGCCCAGCCACGCCGGTGGCCGCCGCAAAACGTGGGGAAGGACGCGCCCAGCCCCCAGCGAGCGCGGGTGGCGGTCGCGGCTGCAGAAGGCGCAtt > 4:426684/1‑149 (MQ=255) gACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATgg < 6:161837/39‑1 (MQ=255) gACGTGTTT‑CG‑CGGGCTGATCCATCGTCAGCCACGATgg > 5:161837/1‑39 (MQ=255) | GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTT‑GTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑CG‑C‑GGCTGATCCATCGTCAGCCACGATGGATCAG‑CCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATT > NC_002947/3845557‑3845834 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |