Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	336,125	+T	coding (210/237 nt)	PP_0278 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	336,124	1	.	T	100.0%	179.7 / NA	54	D70V (GAT→GTT)	PP_0278	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (25/29);  total (25/29)

ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTC  >  NC_002947/335980‑336260                                                                                                                                                  |                                                                                                                                          aCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttac                                                                                                                                       <  5:267408/149‑4 (MQ=255)  cccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacc                                                                                                                                      <  5:188036/149‑5 (MQ=255)        ccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGc                                                                                                                                  <  5:88341/148‑1 (MQ=255)        ccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGc                                                                                                                                  <  6:314511/148‑1 (MQ=255)         cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATGCTGATGGGCTACATCAAGCACCTGCTGCGAGAGGGCGATATGGAGGACGCCCATAAATTCGTGGGGGCAGCCGATTACCTGTGCGa                                                                                                                                >  1:350531/1‑149 (MQ=255)         cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑tt                                                                                                                                         >  8:82868/1‑138 (MQ=255)         cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑tt                                                                                                                                         <  7:82868/139‑2 (MQ=255)            aaTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGAc                                                                                                                               <  8:397403/147‑1 (MQ=255)            aaTCTCCTACGCGTAGAGACCGGCGTCCCCGTCGACGCGGCCAACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGAGCACCATAAATTCCTTGGGGCAGCCGATTACCTGTGCGAc                                                                                                                               >  7:176519/1‑147 (MQ=255)                  cTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATgg                                                                                                                           <  1:195132/145‑1 (MQ=255)                      gcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACTAGCCTGTCTCGATCCTGATGGGCTACATCAATCACCTGGTGCGAGAGGGCGATATGGAGGAAGACCATAAATTCCTGGGGGCAGCGGATTACCTGTGCGACATGGCCAAGgcg                                                                                                                   >  6:328198/1‑149 (MQ=255)                       cgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGgcgc                                                                                                                  <  1:283230/149‑1 (MQ=255)                        gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCt                                                                                                                 >  2:186085/1‑149 (MQ=255)                              cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGaa                                                                                                           >  5:81241/1‑149 (MQ=255)                               ccGGCGTCCCCGTCGACGGGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAAc                                                                                                          <  3:30292/149‑1 (MQ=255)                                  gCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGat                                                                                                       <  1:375944/149‑1 (MQ=255)                                        ccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                    >  8:170090/1‑146 (MQ=255)                                              aCGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGat                                                                                                       <  3:142178/137‑1 (MQ=255)                                              aCGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGat                                                                                                       >  4:142178/1‑137 (MQ=255)                                                gcgGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCaa                                                                                         <  7:170090/149‑1 (MQ=255)                                                         gAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGgc                                                                               >  3:177192/1‑149 (MQ=255)                                                                 ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAgg                                                                       >  3:176763/1‑149 (MQ=255)                                                                  tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAgg                                                                       >  7:296281/1‑148 (MQ=255)                                                                  tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAgg                                                                       >  4:247594/1‑148 (MQ=255)                                                                    gtttcTGATGGGCTACATCAAGCACCTGGTGCGCGATGGCTATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCGAAGGG‑TAAGGCGCATTAgg                                                                       <  2:121434/142‑1 (MQ=255)                                                                     aTCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCAtt                                                                   <  7:138666/149‑1 (MQ=255)                                                                          gATGGGCTACATCAAGCTCCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAgg                                                                                       <  5:372625/125‑1 (MQ=255)                                                                          gATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAgg                                                                                       >  6:372625/1‑125 (MQ=255)                                                                            tGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCgg                                                            <  1:87747/149‑1 (MQ=255)                                                                            tGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCgg                                                            >  8:35507/1‑149 (MQ=255)                                                                             gggCTACATCAAGCACCTGGTGCTCGAGTGCGATATGGAGGACGACCATAAATTCATGGGGGCAGAAGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCTTTGACGCgg                                                            <  8:176519/148‑1 (MQ=255)                                                                               gCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGAAATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCa                                                         >  5:6358/1‑149 (MQ=255)                                                                                   cATCAAGCAGCTGGTGCGCGAGGGCTTTATGGAGGACGACCATAAATTCCTGGTGGCAGCCGATTACCTGTGCGACATGGCCAAGGCTCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCAttt                                                      <  5:328198/148‑1 (MQ=255)                                                                                    aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGAcgc                                                              <  4:413127/139‑1 (MQ=255)                                                                                    aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGAcgc                                                              >  3:413127/1‑139 (MQ=255)                                                                                     tCAAGCACCTGGTGCGCGATGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCCGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGATAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                    <  1:59176/149‑1 (MQ=255)                                                                                     tCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGAAATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                   >  5:21470/1‑149 (MQ=255)                                                                                      cAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                  >  8:235873/1‑149 (MQ=255)                                                                                      cAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                  >  2:379755/1‑149 (MQ=255)                                                                                       aaGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                  <  4:372854/148‑1 (MQ=255)                                                                                        aGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                  >  1:263516/1‑147 (MQ=255)                                                                                           aCCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCg                                             >  3:212285/1‑149 (MQ=255)                                                                                             cTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGc                                           <  6:81241/149‑1 (MQ=255)                                                                                                gTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGcc                                          <  3:247594/147‑1 (MQ=255)                                                                                                gTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGcc                                          <  1:186085/147‑1 (MQ=255)                                                                                                  gcgcGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGc                                      >  7:240624/1‑149 (MQ=255)                                                                                                       aGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTcgc                                 <  7:326263/149‑1 (MQ=255)                                                                                                           cGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAt                             <  4:212285/149‑1 (MQ=255)                                                                                                                     gacgacCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCaaa                   <  7:235873/149‑1 (MQ=255)                                                                                                                     gacgacCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCa                     >  6:97983/1‑147 (MQ=255)                                                                                                                      acgacCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAag                  <  8:240624/149‑1 (MQ=255)                                                                                                                       cgacCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagc                 <  7:35507/149‑1 (MQ=255)                                                                                                                           cATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTAGCGGAATGGCTGCAAAGCAccc             >  5:391093/1‑149 (MQ=255)                                                                                                                                aTTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCTTTGCAGcccat          >  2:102772/1‑145 (MQ=255)                                                                                                                                aTTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCTTTGCAGcccat          <  1:102772/147‑3 (MQ=255)                                                                                                                                     tGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGAcg                                                               >  3:416432/1‑89 (MQ=255)                                                                                                                                     tGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGAcg                                                               <  4:416432/89‑1 (MQ=255)                                                                                                                                      gggggCAGCGGATTACCTGTGGGACATGGCCAAGGGGCTTATGAACGATATCTTTATCGCCATGGG‑TAAGGCGCATTAGGCTTTGACGCGGTCTTTTGTGGGAGCGGCCTTGCGTCGCGATTGGGCTGCAAAGCAGCCCCGGCGATTTc  <  2:263516/149‑1 (MQ=37)                                                                                                                                                  |                                                                                                                                          ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGG‑TAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTC  >  NC_002947/335980‑336260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑