Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	98.3%	193.0 / ‑4.3	58	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (27/30);  minor base C (1/0);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

AGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑AGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAA  >  NC_002947/499059‑499346                                                                                                                                                  |                                                                                                                                                aGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCa                                                                                                                                             >  8:166548/1‑149 (MQ=255)  gACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGGCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCAt                                                                                                                                            >  5:382542/1‑149 (MQ=255)  gACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCAt                                                                                                                                            >  8:383939/1‑149 (MQ=255)  gACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCAt                                                                                                                                            <  8:334287/149‑1 (MQ=255)      aTCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGccc                                                                                                                                        <  4:142260/149‑1 (MQ=255)       tCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCcg                                                                                                                                       <  7:126242/149‑1 (MQ=255)         aGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAATCTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCcgc                                                                                                                                      >  4:60757/1‑148 (MQ=255)           cACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGTCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGaa                                                                                                                                   >  2:151313/1‑149 (MQ=255)              ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGCATCCCCCCCAACg                                                                                                                                 >  1:312507/1‑149 (MQ=255)              ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGTCCGCGAACgg                                                                                                                                <  7:263900/149‑1 (MQ=255)              ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACgg                                                                                                                                >  2:113792/1‑149 (MQ=255)                acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGTCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGggc                                                                                                                              <  1:151313/149‑1 (MQ=255)                acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGggc                                                                                                                              >  7:410145/1‑149 (MQ=255)                  aaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCCTGCCCGCGGACCggcct                                                                                                                            >  3:277367/1‑149 (MQ=255)                   aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGggcctg                                                                                                                           <  3:304785/149‑1 (MQ=255)                        cGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGCAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGgcctggcctg                                                                                                                      <  1:113792/149‑1 (MQ=255)                         gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCctgc                                                                                                                     >  8:291274/1‑149 (MQ=255)                          ccTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCctgct                                                                                                                    >  4:134883/1‑149 (MQ=255)                          ccTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCctgct                                                                                                                    <  7:383939/149‑1 (MQ=255)                           cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGA‑GGGCATTCCCGCGAACGGGCCTGGCctgct                                                                                                                    >  5:208015/1‑148 (MQ=255)                              aagCATAACTTGAAAGTGAACTCTCTGGCCGAGCTGGGGAAGTACCCCATGTAGCACAAGCTGATCTCATTGCGACATACGGGGGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGa                                                                                                                  <  7:113705/145‑1 (MQ=255)                               tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtct                                                                                                               <  3:134883/149‑1 (MQ=255)                                 cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtctc                                                                                                              <  6:248522/148‑1 (MQ=255)                                 cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtctc                                                                                                              >  2:131737/1‑148 (MQ=255)                                 cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtctc                                                                                                              >  1:249360/1‑148 (MQ=255)                                  aGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTa                                                                                                            >  4:302508/1‑149 (MQ=255)                                    aaGTTGAAAGTGAACTCGCTGGTGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGACCGCGAACGGGCCAGGCCGGCGGATCTCTAtt                                                                                                          >  8:57694/1‑148 (MQ=255)                                             gTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCg                                                                                                 <  6:158011/149‑1 (MQ=255)                                                aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                               <  5:119348/148‑1 (MQ=255)                                                         gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTTGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTcgcg                                                                                      <  2:249360/148‑1 (MQ=255)                                                         gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTcgc                                                                                       <  1:131737/147‑1 (MQ=255)                                                         gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                     <  3:60757/149‑1 (MQ=255)                                                                  gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑CGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCg                                                                            >  4:404630/1‑149 (MQ=255)                                                                          cGCCATGTAGCACAAGTTTATCTGATTGCGACATACGGGTGCCCTAAACTTGCCGATCCCTATGTGGTTGC‑GGGAATGCCCGCTAAGGGGCCTGGCCTGCTTATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCcac                                                                     <  6:208015/148‑1 (MQ=37)                                                                          cGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGa                                                                                                                  >  8:148469/1‑103 (MQ=255)                                                                          cGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGa                                                                                                                  <  7:148469/103‑1 (MQ=255)                                                                             cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGGAAGCCCGCGCACCGGCCAGGCCTGGGGATATCTATTGCTGAGCCGGCCGAGTAGCGGGCAATCCCGCGCCCCCACGt                                                                 >  3:257050/1‑149 (MQ=255)                                                                             cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGc                                                                               >  8:93426/1‑135 (MQ=255)                                                                             cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGc                                                                               <  7:93426/135‑1 (MQ=255)                                                                             aaTGGAGCAAAAGGTTATCTTATTGCGACATAGGGGTGCCCTAAAATTGCCTAAGCTTTTGGGGTAGG‑GGGAATGCCCGCGAAGGGGCGTGGCCTGCTGATCTCTATGGCTGAGCGGGCGTCTTCGGGGGAATGCCCGCTCCCACAGGt                                                                 <  6:382542/148‑1 (MQ=255)                                                                                ggAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTc                                                              >  1:49775/1‑149 (MQ=255)                                                                                      caAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGtt                                                        >  6:327783/1‑149 (MQ=255)                                                                                       aaGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCCAGCCCGCGAACGGGCCTGGCCGGGGGAAGACTATTGCGGAGCCGGCCGATTAGCCGGCCTTGCCCCTCCCACAGGTTTAGTGGTTg                                                       >  2:276450/1‑149 (MQ=255)                                                                                          ctgatctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGCGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCt                                                    <  7:166548/149‑1 (MQ=255)                                                                                             atcttatTGCGAAATACGGGTCCCCGAACCTTGCCTAAGCATATTTGGTAGC‑GGGCATGCCCGCTAACGGGCCTGCCCTGCTTATCTCTATGGCTGAGCGGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTc                                                   <  2:295586/147‑1 (MQ=37)                                                                                                      cGACATCCGGGGGCCCCAAACTTGCCGAAGCCTATGGGGGAGC‑GGGCATGCCCGCTAAGGGGCCTGGCGTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCAtt                                        <  2:144751/149‑1 (MQ=38)                                                                                                           tACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTc                                                                         <  2:308726/111‑1 (MQ=255)                                                                                                           tACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTc                                                                         >  1:308726/1‑111 (MQ=255)                                                                                                                gTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCAAGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGc                              >  6:206723/1‑149 (MQ=255)                                                                                                                      gAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCt                        >  6:79862/1‑149 (MQ=255)                                                                                                                          cTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTa                    <  5:79862/149‑1 (MQ=255)                                                                                                                                 aaGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGAt             <  7:291274/149‑1 (MQ=255)                                                                                                                                   gCCTTTGGGGGCGC‑GGGCATGCCCGCCAACGGGCGTGGCGTGCTGATCTCTATGGCTGCGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATcc           <  7:57694/149‑1 (MQ=38)                                                                                                                                          tgGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTacaa     <  3:302508/148‑1 (MQ=255)                                                                                                                                          tgGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTa        <  7:10370/145‑1 (MQ=255)                                                                                                                                             gAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTTGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTAcaacaa  >  2:257482/1‑148 (MQ=255)                                                                                                                                             gAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTacaac    <  2:285825/146‑1 (MQ=255)                                                                                                                                             gAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTacaac    >  1:285825/1‑146 (MQ=255)                                                                                                                                                  |                                                                                                                                                AGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑AGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAA  >  NC_002947/499059‑499346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑