Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,098,177	Δ1 bp	5.2%	coding (1215/2091 nt)	acsA‑I ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,098,177	0	G	.	5.2%	246.0 / 6.0	77	coding (1215/2091 nt)	acsA‑I	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (36/37);  new base . (2/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

TCGACGATCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGT  >  NC_002947/5098031‑5098325                                                                                                                                                    |                                                                                                                                                      tCGACGATCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGCTCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGgcgc                                                                                                                                                      <  3:269099/148‑1 (MQ=255)   gacgaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGaa                                                                                                                                                   >  3:104675/1‑149 (MQ=255)   gacgaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAAACGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGaa                                                                                                                                                   >  6:302855/1‑149 (MQ=255)    acgaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGa                                                                                                                                                    >  4:164977/1‑147 (MQ=255)      gaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                                <  5:310000/149‑1 (MQ=255)       aTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                                >  5:139393/1‑148 (MQ=255)          gggCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAg                                                                                                                                             <  3:182214/148‑1 (MQ=255)          gggCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCATTCGCGCCATGATGGC‑CGAAGGGCAg                                                                                                                                             <  5:316993/148‑1 (MQ=255)           ggCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGtt                                                                                                                                           >  3:116326/1‑149 (MQ=255)              aaCGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑Ggc                                                                                                                                       >  8:28502/1‑149 (MQ=255)               aCGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑Ggcg                                                                                                                                      <  5:35496/149‑1 (MQ=255)                cGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑Ggcgc                                                                                                                                     <  3:164977/149‑1 (MQ=255)                cGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCATTCGCGCCATGATGGC‑CGAAGGGCAggcagctg                                                                                                                                      <  3:26236/149‑8 (MQ=38)                        gCCGACGGTCTTGTAGTACGAGTTCCAGGCCTCAGGGTTGAGCGGCTCACCCACCGTCCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTag                                                                                                                              <  1:210463/148‑1 (MQ=255)                         ccGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTaga                                                                                                                             <  7:262097/148‑1 (MQ=255)                         ccGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTaga                                                                                                                             <  7:83068/148‑1 (MQ=255)                         ccGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTaga                                                                                                                             <  4:104675/148‑1 (MQ=255)                                tCTTGTAGTTGCAGGTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGAGGCAGGCTTGAACGGTCGGCGCCTTCATCAGCTGCCTGCCCTTCG‑GCCATCTTGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTg                                                                                                                     <  4:9388/149‑1 (MQ=255)                                  ttGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGAc                                                                                                                   >  6:25071/1‑149 (MQ=255)                                  ttGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTAAG‑GCCAACATGGCGCGAATGGCACTT‑GGCGCAGTGTAGAGGATGTTGAc                                                                                                                   >  8:310069/1‑149 (MQ=255)                                    gtagtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGAcc                                                                                                                  <  8:114520/148‑1 (MQ=255)                                       gtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTtgt                                                                                                              <  4:116326/149‑1 (MQ=255)                                       gtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTtgt                                                                                                              >  5:80438/1‑149 (MQ=255)                                       gtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTtg                                                                                                               <  2:380872/148‑1 (MQ=255)                                        taCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTtgtg                                                                                                             <  3:110958/149‑1 (MQ=255)                                        taCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTtgt                                                                                                              <  7:310069/148‑1 (MQ=255)                                          ccAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCt                                                                                                           >  4:199552/1‑149 (MQ=255)                                          ccAGTTCCAGGCCTCAGGGGTGATCGGCTCACCGACCGACCCCAGCAGAGGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGc                                                                                                            <  1:223534/148‑1 (MQ=255)                                           cAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTa                                                                                                                               >  6:380172/1‑128 (MQ=255)                                           cAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTa                                                                                                                               <  5:380172/128‑1 (MQ=255)                                                 cAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTcgac                                                                                                    >  4:282669/1‑149 (MQ=255)                                                  aGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTcgac                                                                                                    >  2:116361/1‑148 (MQ=255)                                                     ccTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATc                                                                                                >  8:154613/1‑149 (MQ=255)                                                      cTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCt                                                                                               >  4:143333/1‑149 (MQ=255)                                                      cTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCt                                                                                               >  1:377580/1‑149 (MQ=255)                                                         aGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCtt                                                                                              <  6:139393/147‑1 (MQ=255)                                                            gTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATa                                                                                         <  8:201002/149‑1 (MQ=255)                                                               gATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATAcgc                                                                                      >  4:109982/1‑149 (MQ=255)                                                                   ggCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCggg                                                                                   <  3:282669/148‑1 (MQ=255)                                                                      tCACCCACCGACCCCAGCAGACGCAGGCTGGAAACGTCGGCGCCTTAAACACCTGCCTGCCCATCG‑GCCAAAATGGCGCGAATGGGAGTT‑GGCCCTGTGTAGAGGATGTTGACCTTGTGCTTGTCCACGATCATAGCAACCCGGGTAAt                                                                               >  1:249419/1‑149 (MQ=255)                                                                          ccaccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTc                                                                                             >  4:372389/1‑131 (MQ=255)                                                                          ccaccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTc                                                                                             <  3:372389/131‑1 (MQ=255)                                                                          ccaccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTcc                                                                           >  5:24158/1‑149 (MQ=255)                                                                            accGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCgg                                                                         >  2:277490/1‑149 (MQ=255)                                                                            accGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCgg                                                                         <  5:302855/149‑1 (MQ=255)                                                                            accGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCgg                                                                         <  3:143333/149‑1 (MQ=255)                                                                             cccaCCCCACCATCCGCAGGGGGGAACCGGGGGGGCCTTAAAAACGTGCCTGCCCTTCG‑GCCATAATGGGGGGAATGGCAGTTCGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCgg                                                                         <  1:116361/146‑1 (MQ=255)                                                                                aCCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAg                                                                     >  8:308003/1‑149 (MQ=255)                                                                                aCCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTA‑GCCGCGGTGTAGAGGAAGTTGCCCTAGTGCTTGTCCACGATCTTCGAAACTCGCGTAATGTCCGAGTAg                                                                     >  4:213865/1‑149 (MQ=255)                                                                                  cccAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGtt                                                                   >  4:375056/1‑149 (MQ=255)                                                                                    cagcagACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGc                                                                                                                                               <  2:130067/72‑1 (MQ=255)                                                                                    cagcagACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGc                                                                                                                                               >  1:130067/1‑72 (MQ=255)                                                                                    cagcagACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTgg                                                                 >  3:21965/1‑149 (MQ=255)                                                                                           cGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGcc                                                          >  5:143669/1‑149 (MQ=255)                                                                                                  tGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGGGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGaa                                                    >  1:246347/1‑148 (MQ=255)                                                                                                          tCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAggt                                           <  6:24158/149‑1 (MQ=255)                                                                                                            ggCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCGGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAggtg                                          <  6:80438/148‑1 (MQ=255)                                                                                                             gcgcCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAggtggt                                        >  3:379858/1‑149 (MQ=255)                                                                                                                  ttCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAAt                                                                               <  6:390155/105‑1 (MQ=255)                                                                                                                  ttCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAAt                                                                               >  5:390155/1‑105 (MQ=255)                                                                                                                  ttCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTc                                                       >  5:12645/1‑129 (MQ=255)                                                                                                                  ttCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTc                                                       <  6:12645/129‑1 (MQ=255)                                                                                                                  ttCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGgcgc                                   >  4:287714/1‑149 (MQ=255)                                                                                                                    cAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGcc                                  <  2:292281/148‑1 (MQ=255)                                                                                                                     aaCAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGt                                >  6:51712/1‑149 (MQ=255)                                                                                                                      aCAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGCGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGtt                               <  2:246347/149‑1 (MQ=255)                                                                                                                       cAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTg                              <  3:375056/149‑1 (MQ=255)                                                                                                                       cAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTg                              <  1:277490/149‑1 (MQ=255)                                                                                                                        aGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTgg                             >  1:28103/1‑149 (MQ=255)                                                                                                                               tgccATTCGCGCCATGATGGC‑CGAAGGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCgg                      >  8:349404/1‑149 (MQ=38)                                                                                                                                 cccTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGc                    >  1:388050/1‑149 (MQ=255)                                                                                                                                  ccTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGcc                   >  4:61455/1‑149 (MQ=255)                                                                                                                                  cattcgcgCCATGATGGC‑CGAAGGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGcc                   >  5:111754/8‑149 (MQ=38)                                                                                                                                   cTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCg                  <  3:287714/149‑1 (MQ=255)                                                                                                                                      cG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGt                 <  4:339029/147‑1 (MQ=255)                                                                                                                                      cG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTa                <  5:25071/148‑1 (MQ=255)                                                                                                                                               atGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGAtata       <  7:308003/149‑1 (MQ=255)                                                                                                                                               atGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGACACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGAtata       <  7:154613/149‑1 (MQ=255)                                                                                                                                                    gcgAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGGGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCtgt  <  2:377580/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TCGACGATCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTT‑GGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGT  >  NC_002947/5098031‑5098325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑