Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	564,382	Δ1 bp	18.6%	intergenic (+34/‑139)	rplQ → / → katA	50S ribosomal protein L17/catalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	564,382	0	T	.	18.6%	188.0 / 42.0	70	intergenic (+34/‑139)	rplQ/katA	50S ribosomal protein L17/catalase
Reads supporting (aligned to +/- strand):  ref base T (28/29);  new base . (6/7);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

GGGCGGCTACCTGCGCATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGAGAGCCCATGAGCAAGA  >  NC_002947/564234‑564530                                                                                                                                                      |                                                                                                                                                      gggCGGCTACCTGCGCATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGt                                                                                                                                                       <  8:164850/149‑1 (MQ=255)  ggcggcTACCTGCGCATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTg                                                                                                                                                      >  5:8837/1‑149 (MQ=255)           cTGCGCATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGCACTAG‑GCCCGGtttt                                                                                                                                             <  7:226564/149‑1 (MQ=255)              cgcATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGGCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAAGCGGGCACTAG‑GCCCGGTTTTCTg                                                                                                                                          <  4:337212/149‑1 (MQ=255)                cATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGtt                                                                                                                                        <  1:230163/149‑1 (MQ=255)                 aTCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTTCTACAGAAAACCGGGC‑CTAGTGCCCTGTTTTTTGtt                                                                                                                                        >  8:324917/1‑148 (MQ=255)                  tCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAAACCGGGCACTAG‑GCCCGGttttct                                                                                                                                           >  4:21598/1‑142 (MQ=255)                  tCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAAACCGGGCACTAG‑GCCCGGttttct                                                                                                                                           <  3:21598/144‑3 (MQ=255)                  tCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAAACCGGGCACTAG‑GCCCGGTTTTCTg                                                                                                                                          >  5:32982/1‑145 (MQ=255)                  tCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAAACCGGGCACTAG‑GCCCGGTTTTCTg                                                                                                                                          <  6:32982/145‑1 (MQ=255)                    cTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTtat                                                                                                                                     >  2:282512/1‑148 (MQ=255)                    cTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTtat                                                                                                                                     <  8:163735/148‑1 (MQ=255)                    cTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAAACCGGGCACTAG‑GCCCGGTTTTCTGtagca                                                                                                                                     <  5:84631/148‑5 (MQ=38)                     tGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTtata                                                                                                                                    >  1:80588/1‑148 (MQ=255)                       aaGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGtagcaga                                                                                                                                   <  2:315793/147‑7 (MQ=255)                       aaGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATAc                                                                                                                                   <  7:313929/147‑1 (MQ=255)                               tttCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGtt                                                                                                                         <  6:121837/149‑1 (MQ=255)                                 tCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACAt                                                                                                                                 >  1:161377/1‑139 (MQ=255)                                 tCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACAt                                                                                                                                 <  2:161377/139‑1 (MQ=255)                                        tGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAAACCGGGCACTAG‑GCCCGGTTTTCTGt                                                                                                                                         >  5:112065/1‑124 (MQ=38)                                        tGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAAAAAACCGGGCACTAG‑GCCCGGTTTTCTGt                                                                                                                                         <  6:112065/124‑1 (MQ=38)                                          gCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTAtt                                                                                                              <  3:168714/148‑1 (MQ=255)                                          gCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTAt                                                                                                               <  8:208115/147‑1 (MQ=255)                                            gACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGAt                                                                                                           <  6:61209/149‑1 (MQ=255)                                            gACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGAt                                                                                                           <  2:80588/149‑1 (MQ=255)                                            gACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGAt                                                                                                           <  1:282512/149‑1 (MQ=255)                                                  gcgcCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAg                                                                                                      >  4:131398/1‑148 (MQ=255)                                                      cTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATg                                                                                                 <  6:284800/149‑1 (MQ=255)                                                              tATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAAt                                                                                         <  2:387463/149‑1 (MQ=255)                                                                 gTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTg                                                                                      >  5:75707/1‑149 (MQ=255)                                                                 gTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTg                                                                                      >  7:84613/1‑149 (MQ=255)                                                                  tCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGt                                                                                     <  6:8837/149‑1 (MQ=255)                                                                      gCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCt                                                                                 >  2:291039/1‑149 (MQ=255)                                                                          gTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTaa                                                                             <  3:131398/149‑1 (MQ=255)                                                                          gTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTaa                                                                             >  1:263256/1‑149 (MQ=255)                                                                                     ggTCGGCGGTGCTGTATTAGCTGGTTAGTAAGAAGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTTTTTATACATCTATTGTTTACTTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGt                                                                   <  1:291039/149‑1 (MQ=255)                                                                                     ggTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTaa                                                                             <  4:377242/138‑1 (MQ=255)                                                                                     ggTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTaa                                                                             >  3:377242/1‑138 (MQ=255)                                                                                      gTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCa                                                                 <  6:75707/149‑1 (MQ=255)                                                                                          gcggTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATAc                                                             >  8:147918/1‑149 (MQ=255)                                                                                          gcggTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATAc                                                             >  8:139301/1‑149 (MQ=255)                                                                                                  gTAGAAGCTGCTGAGTATGAAGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATActctctct                                                      <  7:324917/148‑1 (MQ=255)                                                                                                     gAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGCAATCTTGCTCGTCAATACTCTCTCTa                                                     <  7:341479/146‑1 (MQ=255)                                                                                                     gAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGCAATCTTGCTCGTCAATACTCTCTCTa                                                     >  8:341479/1‑146 (MQ=255)                                                                                                      aaGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CAAGTGAACGGGTTTTTGTTTATCCATCTATTGTTGA‑TTTCGAGTGATGTAAGTCATTTAATTAATTTGTCCCTGTAATCTTGCTCGTCACTACTCTCGCTACacc                                                 >  4:243156/1‑147 (MQ=255)                                                                                                           gctgAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGCAATCTTg                                                                        <  8:187376/121‑1 (MQ=255)                                                                                                           gctgAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGCAATCTTg                                                                        >  7:187376/1‑121 (MQ=255)                                                                                                             tgAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAg                                          >  3:80896/1‑149 (MQ=255)                                                                                                                gTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATATATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGc                                         <  8:115835/147‑1 (MQ=255)                                                                                                                gTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGc                                         >  7:115835/1‑147 (MQ=255)                                                                                                                 tAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCgg                                      >  7:142781/1‑149 (MQ=255)                                                                                                                 tAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCgg                                      >  6:40644/1‑149 (MQ=255)                                                                                                                   aGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTATCTACATCCGATTAGCCGGcc                                    >  6:218578/1‑149 (MQ=255)                                                                                                                    gACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATAc                                                             >  2:102333/1‑123 (MQ=255)                                                                                                                    gACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATAc                                                             <  1:102333/123‑1 (MQ=255)                                                                                                                    gACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGccc                                   >  8:19378/1‑149 (MQ=255)                                                                                                                                 cTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGa                        <  7:147918/147‑1 (MQ=255)                                                                                                                                  aaCAAAAAACCGGGCACTAG‑GCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGAcc                          >  7:333428/2‑144 (MQ=255)                                                                                                                                  aaCAAAAAACCGGGCACTAG‑GCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGAcc                          <  8:333428/143‑1 (MQ=255)                                                                                                                                     aaaaaaCCGGGCACTAG‑GCCCGGTTTTCTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATg                    >  5:77427/3‑147 (MQ=255)                                                                                                                                     aaaaaaCCGGGCACTAG‑GCCCGGGTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGATCGGCAATACTATCTCTACAGCAGATTAGCCGGCCCTGGAAGACCGAAATg                    >  2:93147/3‑147 (MQ=255)                                                                                                                                     aGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGga                  <  7:19378/149‑1 (MQ=255)                                                                                                                                     aGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTAAATACTCTCTCTACAGCCGAGTAGCCGGCCGTTGAAGACCGAAATg                    >  8:97442/1‑147 (MQ=255)                                                                                                                                      gAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTc                                                                     <  7:254854/97‑1 (MQ=255)                                                                                                                                      gAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTc                                                                     >  8:254854/1‑97 (MQ=255)                                                                                                                                      gAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCATGTAATATTGATCGTCTATACTGTCTCTACAGCAGATTATAAGGAACTTGTAGTCCTAAATGgag                 >  5:292104/1‑149 (MQ=255)                                                                                                                                       aaaaCCGGGC‑CTAGTGGCCGGTTTTTTGTTTATAGATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGgaga                <  8:84613/149‑1 (MQ=255)                                                                                                                                         aaCCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGAGAGc              >  3:351634/1‑149 (MQ=255)                                                                                                                                           ccGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGAGAGccc            <  5:40644/149‑1 (MQ=255)                                                                                                                                           ccGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGAGAGcc             >  5:168206/1‑148 (MQ=255)                                                                                                                                             gggC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGAGAGCCCAt          <  1:154578/149‑1 (MQ=255)                                                                                                                                                      tGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGAGAGCCCATGAGCAAGa  <  5:310575/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      GGGCGGCTACCTGCGCATCCTGAAGTGCGGTTTCCGCGCTGGCGACAACGCGCCTATGGCGTATGTCGAGCTGGTTGATCGTCCGGTCGGCGGTGCTGTAGAAGCTGCTGAGTAAGACGTTCTGTCTGCTACAGAAAACCGGGC‑CTAGTGCCCGGTTTTTTGTTTATACATCTATTGTTTA‑TTTCTATTGATGTAAGTCATGTAATGAATTTGTCCCTGTAATCTTGCTCGTCAATACTCTCTCTACAGCCGATTAGCCGGCCCTTGAAGACCGAAATGGAGAGCCCATGAGCAAGA  >  NC_002947/564234‑564530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑