Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,426,626 Δ1 bp 12.8% coding (268/942 nt) PP_2127 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,426,6260C.12.8% 137.5 / 15.0 47coding (268/942 nt)PP_2127sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base . (3/3);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01

CCTGCAACTCGTCGGGCATGCGCCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCA  >  NC_002947/2426482‑2426769
                                                                                                                                                 |                                                                                                                                               
ccTGCAACTCGTCGGGCATGCGCCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGcc                                                                                                                                             >  6:251918/1‑149 (MQ=255)
 cTGCAACTCGTCGGGCATGCGCCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCg                                                                                                                                            >  1:223509/1‑149 (MQ=255)
       cTCGTCGGGCCAGTGCCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTCCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCGCCTTGAGCATGCGCCCGGCCGAGCT‑GGCCGAGCGCa                                                                                                                                      <  1:329127/149‑1 (MQ=255)
          gtcgGGCATGCGCCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAgg                                                                                                                                    <  4:43212/148‑1 (MQ=255)
          gtcgGGCATGCGCCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGt                                                                                                                                   <  5:251918/149‑1 (MQ=255)
          gtcgGGCATGCGCCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCCCGGCCGAGCT‑GGCCGAGCGCat                                                                                                                                     <  7:273296/147‑2 (MQ=255)
          gtcgGGCATGCGCCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCCCGGCCGAGCT‑GGCCGAGCGCat                                                                                                                                     <  4:44143/147‑2 (MQ=255)
                              gCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTTGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCgcccagg                                                                                                               <  1:88468/149‑1 (MQ=255)
                                cACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCgcccaggcc                                                                                                             >  4:18911/1‑149 (MQ=255)
                                      aGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTg                                                                                                       <  7:213885/149‑1 (MQ=255)
                                      aGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTg                                                                                                       <  4:341592/149‑1 (MQ=255)
                                        ttGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTa                                                                                                     <  2:13474/149‑1 (MQ=255)
                                        ttGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTa                                                                                                     <  3:18911/149‑1 (MQ=255)
                                            aGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTacaac                                                                                                 <  6:211483/149‑1 (MQ=255)
                                            aGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTacaa                                                                                                  >  8:355209/1‑148 (MQ=255)
                                            aGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTacaa                                                                                                  >  2:69135/1‑148 (MQ=255)
                                             gCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTTCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGAGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTTTacaaca                                                                                                <  8:322106/149‑1 (MQ=255)
                                             gCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTacaa                                                                                                  >  6:351525/1‑147 (MQ=255)
                                                           aGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTgg                                                                                   <  7:286727/148‑1 (MQ=255)
                                                                ttGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGGC‑AGCTCGCCCGGGCGCACGTGGAACGACCTGGCGCCCCGGCCCAGTTGTACAACAGCACCCACCGTGGCGAcc                                                                              >  2:229472/1‑148 (MQ=255)
                                                                  gACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCaa                                                                            <  2:223509/148‑1 (MQ=255)
                                                                    ccGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTg                                                                         <  5:351525/149‑1 (MQ=255)
                                                                           cgtcgtGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTTCAACAGCACCCAGCGAGGCGACGAACTGCAGCAcc                                                                  >  7:184392/1‑149 (MQ=255)
                                                                             tcgtGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACcag                                                                >  1:6864/1‑149 (MQ=255)
                                                                               gtGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACcagca                                                              <  7:355209/149‑1 (MQ=255)
                                                                                tGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACcagcag                                                             >  1:87908/1‑149 (MQ=255)
                                                                                 gCAGTTACCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAgg                                                            >  2:339055/1‑149 (MQ=255)
                                                                                       cccGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGcc                                                      >  6:253076/1‑149 (MQ=255)
                                                                                          ggCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCggg                                                   <  2:87908/149‑1 (MQ=255)
                                                                                               aGATGCTGGCGCTCGTCTTCTTGCAACTTGAGCCTGCGCTCGTCC‑ATCTCGGCCGTGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCTTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGc                                              <  5:44003/149‑1 (MQ=255)
                                                                                                  tGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTg                                           <  6:210490/149‑1 (MQ=255)
                                                                                                    cTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGt                                         <  1:234874/149‑1 (MQ=255)
                                                                                                    cTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGt                                         >  2:234874/1‑149 (MQ=255)
                                                                                                    cTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGACC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTgg                                          <  7:152288/148‑1 (MQ=255)
                                                                                                       gcgcTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGtt                                        <  6:222540/147‑1 (MQ=255)
                                                                                                               cttcttGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTa                               >  3:333705/1‑148 (MQ=255)
                                                                                                                         cTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGt                    <  5:253076/149‑1 (MQ=255)
                                                                                                                            gAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTg                  <  2:353060/148‑1 (MQ=255)
                                                                                                                               cATGCGCTCGGCC‑AGCTCGTCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCCACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGtt                   >  6:64147/1‑144 (MQ=255)
                                                                                                                               cATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGtt                   <  5:64147/144‑1 (MQ=255)
                                                                                                                                ctGCGCCCGGCCGAGCT‑GGCCGAGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAAt             >  8:123901/2‑149 (MQ=255)
                                                                                                                                  gcgcCCGGCCGAGCT‑GGCCGAGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTTGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAAt             >  2:241445/1‑147 (MQ=255)
                                                                                                                                  gcgcCCGGCCGAGCT‑GGACGAGCGCAGGTGGATCGACCTGGCGAACAGGCCCAGTTGTACAACATCACACAGCGTGGAGACCAACTGCAGCACCAGCAGGCTGGACGGGATTGCCTGGTTGTCCAGGTACAGCGCCATGTTGGCAAt             >  2:246804/1‑147 (MQ=255)
                                                                                                                                    gcTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGt         >  6:97504/1‑149 (MQ=255)
                                                                                                                                        cgCC‑AGCTCGGCCGGGCGCAGATGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAgg     <  2:167440/148‑1 (MQ=255)
                                                                                                                                         gCC‑AGCTCGGCCGGGCGCAGGTGGATCAACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGc    >  8:147502/1‑149 (MQ=255)
                                                                                                                                           c‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCa  >  2:136419/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCTGCAACTCGTCGGGCATGCGCCGTTGCAGCCACTGCAGTTGCAGCTTGGCAGCGGTGAGCAGTTGACCGATGTCGTCGTGCAGTTCCCGGCCCAGATGCTGGCGCTCGTCTTCTTGCACCTTGAGCATGCGCTCGGCC‑AGCTCGGCCGGGCGCAGGTGGATCGACCTGGCGCCCAGGCCCAGTTGTACAACAGCACCCAGCGTGGCGACCAACTGCAGCACCAGCAGGCTGGCCGGGATTGCCTGGTTGGCCAGGTACAGCGCCATGTTGGCAATCAGTGAGGCCA  >  NC_002947/2426482‑2426769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑