Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,408,047	Δ1 bp	5.8%	coding (497/615 nt)	PP_3021 ←	LysE family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,408,047	0	A	.	5.8%	212.8 / 7.1	69	coding (497/615 nt)	PP_3021	LysE family transporter
Reads supporting (aligned to +/- strand):  ref base A (30/35);  new base . (2/2);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01

CTTGGTCGACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGAC  >  NC_002947/3407900‑3408193                                                                                                                                                    |                                                                                                                                                          cTTGGTCGACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCCCCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑t                                                                                                                                                       >  4:113545/1‑149 (MQ=255) cTTGGTCGACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑t                                                                                                                                                       >  6:162168/1‑149 (MQ=255)    ggTCGACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑Tg                                                                                                                                                      <  2:138955/147‑1 (MQ=255)        gACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑Cg                                                                                                                                             >  7:163129/1‑149 (MQ=255)         aCATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCAAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑Cgg                                                                                                                                            >  4:172987/1‑149 (MQ=255)          cATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGc                                                                                                                                           <  3:369380/149‑1 (MQ=255)             gcgcACCTTGCAACTAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGAc                                                                                                                                        >  7:328787/1‑149 (MQ=255)              cgcACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGAc                                                                                                                                        <  8:280357/148‑1 (MQ=255)                   cTTGCAACAAGTCAGCCGCGGGCCAGATGCAGCAACAACGCCCCCGCCTCCATCACCCGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGGTGCCTA‑‑‑TG‑‑GCCA‑CGGCGACCAGCaa                                                                                                                                  <  4:52647/149‑1 (MQ=255)                   cTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGcgg                                                                                                                                  <  4:143978/149‑3 (MQ=255)                    ttGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAg                                                                                                                                 >  8:270320/1‑149 (MQ=255)                      gCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGt                                                                                                                                <  3:265716/148‑1 (MQ=255)                        aacaaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCa                                                                                                                                      >  4:2599/1‑140 (MQ=255)                        aacaaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCa                                                                                                                                      <  3:2599/140‑1 (MQ=255)                          caaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCag                                                                                                                           >  1:381827/1‑149 (MQ=255)                           aaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCaga                                                                                                                          <  8:212652/149‑1 (MQ=255)                                 gCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑a                                                                                                                   <  7:119047/149‑1 (MQ=255)                                          cAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGa                                                                                                          >  6:306739/1‑149 (MQ=255)                                            gATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAAc                                                                                                        <  7:221305/149‑1 (MQ=255)                                                cagcaACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATc                                                                                                     <  3:366414/148‑1 (MQ=255)                                                  gcaACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCa                                                                                                   >  1:158110/1‑148 (MQ=255)                                                      caacGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCTCTCCAGCCACGCCAGGCTGCGTGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAgcg                                                                                              <  5:299805/149‑1 (MQ=255)                                                      caacGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAgcg                                                                                              <  1:133069/149‑1 (MQ=255)                                                       aacgccaacgccAACATCACCACCATGACCCCCGAGGCCCGCACCAGCCACGCCAAGCAGCGGGCGAAGCGCCGCAGAACCCCCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCACCAACTACCAGAGCAAG‑ACGATGCTGAACAACCATAgcgc                                                                                             >  5:273811/1‑149 (MQ=255)                                                         cGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGcgcg                                                                                            >  6:288849/1‑148 (MQ=255)                                                            ccccGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCTAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAgg                                                                                        <  5:299921/149‑1 (MQ=255)                                                            ccccGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAgg                                                                                        >  2:211511/1‑149 (MQ=255)                                                               cGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCg                                                                                     >  3:133610/1‑149 (MQ=255)                                                                gCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGt                                                                                    >  1:29018/1‑149 (MQ=255)                                                                 ccAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGtt                                                                                   <  8:163129/149‑1 (MQ=255)                                                                      aTCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTc                                                                                >  2:263305/1‑147 (MQ=255)                                                                          ccAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGGTTTCCAGCCg                                                                          >  1:49137/1‑149 (MQ=255)                                                                             gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGTGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       <  6:273811/149‑1 (MQ=255)                                                                             gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       <  5:306739/149‑1 (MQ=255)                                                                             gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       <  1:211511/149‑1 (MQ=255)                                                                             gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       <  5:162168/149‑1 (MQ=255)                                                                              cATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGc                                                                      <  1:62115/149‑1 (MQ=255)                                                                                tGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCAAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCACG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTg                                                                    <  1:248803/149‑1 (MQ=255)                                                                                    ccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       >  7:353242/1‑142 (MQ=255)                                                                                    ccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       <  8:353242/142‑1 (MQ=255)                                                                                    ccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGc                                                                >  5:42740/1‑149 (MQ=255)                                                                                      ccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTg                                                              <  2:49137/149‑1 (MQ=255)                                                                                       cGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGCTATCGATTCTGTTGCAA‑GGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGAc                                                                  <  1:126909/149‑1 (MQ=255)                                                                                         aGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CCGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGc                                                            >  6:23046/1‑148 (MQ=255)                                                                                            cccGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGAc                                                         >  2:324329/1‑148 (MQ=255)                                                                                                cTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGc                                                    <  2:29018/149‑1 (MQ=255)                                                                                                      ccACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCTGCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGcgg                                                                                                                                  >  2:194208/1‑64 (MQ=17)                                                                                                      ccACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCTGCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGcgg                                                                                                                                  <  1:194208/66‑3 (MQ=17)                                                                                                       cACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAg                                             >  7:173617/1‑149 (MQ=255)                                                                                                        aCGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAgg                                            >  7:75051/1‑149 (MQ=255)                                                                                                          gCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTg                                                              <  2:149884/129‑1 (MQ=255)                                                                                                          gCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTg                                                              >  1:149884/1‑129 (MQ=255)                                                                                                               gCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCAt                                     <  6:93209/149‑1 (MQ=255)                                                                                                                     ggCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCATG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACa                               <  3:172987/149‑1 (MQ=255)                                                                                                                       cGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAgc                             <  7:270320/149‑1 (MQ=255)                                                                                                                           gcgcCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGTACGATGCTGAACATCCATAGCGGGTAGGCGGTTTTCCAGCCGGGGATGACGGTGGGGACCATGCTGGCCAGGCTGGCATAGAACAGggcg                          >  1:112115/1‑149 (MQ=255)                                                                                                                           gcgcCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCAAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGt                         >  5:274891/1‑149 (MQ=255)                                                                                                                             gccgcAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTc                       >  5:201797/1‑149 (MQ=255)                                                                                                                                 cAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgg                   <  5:23046/149‑1 (MQ=255)                                                                                                                                  aGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTggg                  <  4:193433/149‑1 (MQ=255)                                                                                                                                   gTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgggg                 <  1:263305/149‑1 (MQ=255)                                                                                                                                   gTACCCACTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgggg                 >  4:123538/1‑149 (MQ=255)                                                                                                                                      cccGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGtt               <  5:288849/148‑1 (MQ=255)                                                                                                                                      cccGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTg              <  2:381827/149‑1 (MQ=255)                                                                                                                                       ttgCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGtt               >  3:370751/3‑147 (MQ=255)                                                                                                                                             gTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGc       >  4:69637/1‑149 (MQ=255)                                                                                                                                              ttGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGcc      <  6:201797/149‑1 (MQ=255)                                                                                                                                                gCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCa     <  2:158110/148‑1 (MQ=255)                                                                                                                                                   gA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCCTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGAc  >  7:343847/1‑148 (MQ=255)                                                                                                                                                    a‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAgc                             <  6:241415/120‑1 (MQ=255)                                                                                                                                                    a‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAgc                             >  5:241415/1‑120 (MQ=255)                                                                                                                                                    |                                                                                                                                                          CTTGGTCGACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGAC  >  NC_002947/3407900‑3408193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑