Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,625,544 Δ1 bp 6.3% intergenic (‑57/+20) PP_3195 ← / ← PP_3196 hypothetical protein/nodulation protein d1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,625,5440T. 6.3% 185.2 / 7.2 63intergenic (‑57/+20)PP_3195/PP_3196hypothetical protein/nodulation protein d1
Reads supporting (aligned to +/- strand):  ref base T (26/33);  new base . (2/2);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

CGGTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCAC  >  NC_002947/3625399‑3625688
                                                                                                                                                  |                                                                                                                                                 
cGGTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTg                                                                                                                                                <  7:19963/149‑1 (MQ=255)
        gAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAGACACCGCAGCGGTG‑CTGGGGcatcg                                                                                                                                        <  1:174771/149‑5 (MQ=255)
           tCCGGCGCCCACTTGAGCGACAGGCAGGCAGGTCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCCGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGgcc                                                                                                                                     <  6:130885/149‑1 (MQ=255)
             cGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGgccgc                                                                                                                                   >  6:122069/1‑149 (MQ=255)
             cGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGgccgc                                                                                                                                   >  5:49943/1‑149 (MQ=255)
                   ccACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCt                                                                                                                               >  6:187479/1‑147 (MQ=255)
                     aCTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAgg                                                                                                                           >  3:77467/1‑149 (MQ=255)
                              aCAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGgcgc                                                                                                                  <  6:234840/149‑1 (MQ=255)
                                 ggcaggcaggGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCa                                                                                                               <  8:44421/149‑1 (MQ=255)
                                  gcgggcaggGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCGGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGACGCCTCTCAGGAGGTGgcgcg                                                                                                                 >  3:37016/4‑146 (MQ=255)
                                   caggcaggGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCaga                                                                                                             <  5:372146/149‑1 (MQ=255)
                                    aggcaggGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCagag                                                                                                            <  5:42962/149‑1 (MQ=255)
                                      gcaggGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAACCGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCa                                                                                                          >  4:141135/1‑149 (MQ=255)
                                         gggCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCt                                                                                                       <  7:356486/149‑1 (MQ=255)
                                          ggCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTg                                                                                                      >  2:164945/1‑149 (MQ=255)
                                           gcgcGGTTTGAATGCAAGGTACGCACGCAGGGCAGGGTGGGTCCAATCGTGATCGGGGATCCGAGGGATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTgg                                                                                                     <  4:37016/149‑1 (MQ=255)
                                             gcgGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCt                                                                                                   <  6:343453/149‑1 (MQ=255)
                                                gTTTGCAGGCAAGGTACGAAGTCACGGCAGGGTGGGTCCAATCCTGATCAGGGATTCGAGTTATCGAGATGCGCGATGCCCCAG‑CACCGCTGCTGTGTCTGCGGCGATGGCAGAAGCTCATGCGGTGCCGCACAGAGCATCTGGCtcag                                                                                                >  6:189657/1‑146 (MQ=255)
                                                     aaGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTg                                                                                           >  6:52511/1‑149 (MQ=255)
                                                       ggCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGgcgcgc                                                                                                                >  5:88034/1‑126 (MQ=255)
                                                       ggCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGgcgcgc                                                                                                                <  6:88034/126‑1 (MQ=255)
                                                       ggCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGgc                                                                                         >  7:17552/1‑149 (MQ=255)
                                                       ggCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGgc                                                                                         >  8:45673/1‑149 (MQ=255)
                                                       ggCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGAGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGATCAGGCGGTGGCTCGCATAGCATATGGCTGCGTGCTGgc                                                                                         >  8:108957/1‑149 (MQ=255)
                                                          aaGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGGTGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCa                                                                                      >  1:74419/1‑149 (MQ=255)
                                                                cggacgcagggcaggGTGGGGCCAATCGTGATCCGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGaa                                                                                 <  8:360853/143‑1 (MQ=255)
                                                                    ggcagggcagGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACa                                                                            <  7:45673/149‑1 (MQ=255)
                                                                           cagggTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACt                                                                                    >  1:292439/1‑134 (MQ=255)
                                                                           cagggTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACt                                                                                    <  2:292439/134‑1 (MQ=255)
                                                                                       aaTCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑c                                                                         <  4:233499/132‑1 (MQ=255)
                                                                                       aaTCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑c                                                                         >  3:233499/1‑132 (MQ=255)
                                                                                       aaTCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGaa                                                        <  5:122069/149‑1 (MQ=255)
                                                                                       aaTCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGaa                                                        <  4:172484/149‑1 (MQ=255)
                                                                                         tCGTGATCATTGATGCGAGGTATCTCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGc                                                      <  6:9057/149‑1 (MQ=255)
                                                                                            tGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCAt                                                   <  6:49943/149‑1 (MQ=255)
                                                                                              aTCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCt                                                 >  8:167881/1‑149 (MQ=255)
                                                                                              aTCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCt                                                 >  2:301233/1‑149 (MQ=255)
                                                                                                          aGGTAGCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCTATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACGGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTc                                     <  6:282756/149‑1 (MQ=255)
                                                                                                             tATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGcc                                  <  7:108957/149‑1 (MQ=255)
                                                                                                             tATCGCGAAACGCGATGCCCCAG‑CACAGCAGCGGTGTCTGGGGCGATTGCCGCCGCTCAGGCGGTGACGAGCAGAGCATCTGGATGCGTGCTGGCGCACTGAATGACAT‑AGCTGATGCGCTCGCGAAGCAATCTGTGCATGGGGTCGcc                                  >  8:354376/1‑149 (MQ=255)
                                                                                                               tCGCGAAACGCGATGCCCCAG‑CACCACTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGt                                >  7:317550/1‑149 (MQ=255)
                                                                                                                 gcgAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCg                                 >  2:346692/1‑146 (MQ=255)
                                                                                                                 gcgAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCg                                 <  1:346692/146‑1 (MQ=255)
                                                                                                                 gcgAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTc                               >  6:15841/1‑148 (MQ=255)
                                                                                                                     aaCGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCAtgc                          >  3:3458/1‑149 (MQ=255)
                                                                                                                         cgATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCg                      <  6:151824/149‑1 (MQ=255)
                                                                                                                            tGCGCCAG‑CTCCGCTGCGGTGTGTTGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGTGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTg                   <  4:77467/149‑1 (MQ=255)
                                                                                                                            tGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTg                   <  3:141135/149‑1 (MQ=255)
                                                                                                                              ccccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGc                  <  1:293329/148‑1 (MQ=255)
                                                                                                                               cccagacACCGCAGCGGTG‑CTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTCTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                >  7:340661/7‑149 (MQ=255)
                                                                                                                               cccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                <  5:52511/149‑1 (MQ=255)
                                                                                                                                ccagacACCGCAGCGGTG‑CTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                >  4:237001/6‑148 (MQ=255)
                                                                                                                                ccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                <  1:301233/149‑1 (MQ=255)
                                                                                                                                ccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGc                                                                       <  1:109715/93‑1 (MQ=255)
                                                                                                                                ccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGc                                                                       >  2:109715/1‑93 (MQ=255)
                                                                                                                                  aG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcaca              <  5:156636/148‑1 (MQ=255)
                                                                                                                                  aG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑AGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                >  6:156636/1‑146 (MQ=255)
                                                                                                                                   gacACCGCAGCGGTG‑CTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGc            <  3:237001/147‑1 (MQ=255)
                                                                                                                                      aCCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt          <  7:167881/149‑1 (MQ=255)
                                                                                                                                       ccGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt          >  4:159481/1‑148 (MQ=255)
                                                                                                                                        cGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGa        <  1:164945/149‑1 (MQ=255)
                                                                                                                                             cGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGTGGCACCGCAGCATggacgg    <  8:17552/148‑6 (MQ=255)
                                                                                                                                              ggTGTCTGGGGCGGTGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCAc  <  2:74419/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGGTCGTGGAGTCCGGCGCCCACTTGAGCGACAGGCAGGCAGGGCGCGGTTTGAAGGCAAGGTACGCAGGCAGGGCAGGGTGGGGCCAATCGTGATCAGGGATGCGAGGTATCGCGAAACGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACAT‑CGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCAC  >  NC_002947/3625399‑3625688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑