Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,962,576	Δ1 bp	8.8%	coding (774/1152 nt)	PP_3492 →	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,962,576	0	A	.	8.8%	202.2 / 13.3	68	coding (774/1152 nt)	PP_3492	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base . (3/3);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGC  >  NC_002947/3962429‑3962721                                                                                                                                                     |                                                                                                                                                   tACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCAt                                                                                                                                                   <  2:273815/149‑1 (MQ=255)   ccTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTc                                                                                                                                                 <  5:280886/149‑1 (MQ=255)    cTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGAATCGCCCGCGCCGCATTc                                                                                                                                                 >  5:39197/1‑148 (MQ=255)          ggTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGaatgcgg                                                                                                                                         >  5:54786/1‑144 (MQ=255)           gTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGACTTCgcgccga                                                                                                                                          >  3:6780/1‑142 (MQ=255)           gTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCAAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCTCCCGCGCCGCATTCGAA‑gcggc                                                                                                                                        <  4:369918/149‑1 (MQ=255)            tCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑gcgg                                                                                                                                         <  2:185496/147‑1 (MQ=255)               ccTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTg                                                                                                                                    <  6:62001/149‑1 (MQ=255)               ccTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGC‑TTCGAATGCGgcgcgg                                                                                                                                    <  1:50025/149‑3 (MQ=255)                 ttCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGt                                                                                                                                  >  6:282345/1‑149 (MQ=255)                    gACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGAATGCGGCGCgggcgat                                                                                                                               <  6:54786/149‑5 (MQ=255)                     aCAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTACCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTAc                                                                                                                              <  1:58786/149‑1 (MQ=255)                           gCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGt                                                                                                                        >  1:63853/1‑149 (MQ=255)                            ccGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGt                                                                                                                        >  2:6715/1‑148 (MQ=255)                            ccGCATGCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGt                                                                                                                        <  3:76248/148‑1 (MQ=255)                             cGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGa                                                                                                                      >  5:390590/1‑149 (MQ=255)                                         gccAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGtt                                                                                                          >  6:1850/1‑149 (MQ=255)                                          ccAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGttt                                                                                                         <  2:250963/149‑1 (MQ=255)                                             atatCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGc                                                                                                      <  6:257407/149‑1 (MQ=255)                                              tatCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCa                                                                                                     <  2:362081/149‑1 (MQ=255)                                               atCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGc                                                                                                      >  1:295663/1‑148 (MQ=255)                                                tCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGGAAg                                                                                                   >  8:240659/1‑149 (MQ=255)                                                 ccTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGAATGCGGCGCggg                                                                                                                                   <  1:138152/116‑1 (MQ=38)                                                 ccTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGAATGCGGCGCggg                                                                                                                                   >  2:138152/1‑116 (MQ=255)                                                 ccTCGGTGAGCGTGGCAAGGGCCTGGCCATCGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGc                                                                                                  >  8:211858/1‑149 (MQ=255)                                                     ggggcgCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCg                                                                                                <  2:295663/142‑1 (MQ=255)                                                     ggTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGAt                                                                                              <  2:63853/149‑1 (MQ=255)                                                         gcaatGGCCAGGCCCTTGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                          >  3:265659/5‑149 (MQ=255)                                                         aGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                          <  4:186606/149‑1 (MQ=255)                                                         aGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                          <  1:6715/149‑1 (MQ=255)                                                            gTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGaa                                                                                       <  8:5145/149‑1 (MQ=255)                                                               gCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGCacac                                                                                    >  2:126901/1‑149 (MQ=255)                                                                 aaGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCa                                                                                  >  2:43609/1‑149 (MQ=255)                                                                       cTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGa                                                                                               <  6:96040/130‑1 (MQ=255)                                                                       cTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGa                                                                                               >  5:96040/1‑130 (MQ=255)                                                                        tGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCAtt                                                                           >  8:209775/1‑149 (MQ=255)                                                                          gCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGc                                                                         >  6:335110/1‑149 (MQ=255)                                                                                 cccTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTg                                                                                                        >  2:214114/1‑111 (MQ=255)                                                                                 cccTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTg                                                                                                        <  1:214114/111‑1 (MQ=255)                                                                                    tGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCAtt                                                                           <  7:177340/137‑1 (MQ=255)                                                                                    tGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCAtt                                                                           >  8:177340/1‑137 (MQ=255)                                                                                      tCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTgg                                                              <  6:39197/148‑1 (MQ=255)                                                                                       ccAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                            >  2:388596/1‑149 (MQ=255)                                                                                         aaCCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGa                                                          >  8:146365/1‑149 (MQ=255)                                                                                           ccTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACa                                                        <  1:200639/149‑1 (MQ=255)                                                                                            cTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAt                                                       >  7:67822/1‑149 (MQ=255)                                                                                               gAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgc                                                     <  1:43609/148‑1 (MQ=255)                                                                                                aa‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgcag                                                   >  6:33652/1‑149 (MQ=255)                                                                                                       gcCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgcaggtgcag                                             <  5:1850/149‑1 (MQ=255)                                                                                                         cGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTAGAA‑GCGGCGCTGGTCTACTCGCGAGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCAATGCCAACCTGCTGGCCGACATtcaggtgcaggt                                           >  4:80740/1‑149 (MQ=255)                                                                                                                      ccgccCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGt                              >  5:387396/1‑149 (MQ=255)                                                                                                                        gccCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGttt                            <  7:209775/149‑1 (MQ=255)                                                                                                                         cccAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTg                           >  5:204597/1‑149 (MQ=255)                                                                                                                           cAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCt                         >  6:379310/1‑149 (MQ=255)                                                                                                                               ccTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATc                     <  5:33652/149‑1 (MQ=255)                                                                                                                                 ttGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAAc                                                                     >  7:299375/1‑99 (MQ=255)                                                                                                                                 ttGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAAc                                                                     <  8:299375/99‑1 (MQ=255)                                                                                                                                 ttGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTAGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCt                   >  8:159900/1‑149 (MQ=255)                                                                                                                                   gggCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCt                   <  8:67822/147‑1 (MQ=255)                                                                                                                                    ggCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGc                 <  6:387396/148‑1 (MQ=255)                                                                                                                                    ggCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGc                 <  7:211858/148‑1 (MQ=255)                                                                                                                                     gCAGCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCAt               <  5:394430/149‑1 (MQ=255)                                                                                                                                           cccGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATgccgcc         >  2:201526/1‑149 (MQ=255)                                                                                                                                           cccGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATgccgcc         >  8:277629/1‑149 (MQ=255)                                                                                                                                            cagcgcCGC‑TTCGAATGCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGccgccc        >  7:123736/3‑149 (MQ=255)                                                                                                                                             cgcgCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGccgcccg       <  7:146365/149‑1 (MQ=255)                                                                                                                                                  cgcATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTgc  >  1:204761/1‑149 (MQ=255)                                                                                                                                                   gcATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGc                          <  6:6403/124‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TACCTGCGCGGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGC  >  NC_002947/3962429‑3962721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑