Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	336,124:1	+T	100%	coding (209/237 nt)	PP_0278 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	336,124	1	.	T	100.0%	194.8 / NA	58	D70V (GAT→GTT)	PP_0278	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (27/31);  total (27/31)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335979‑336263                                                                                                                                                     |                                                                                                                                            aaCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGA‑tt                                                                                                                                           <  1:77854/148‑2 (MQ=255)   cccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGA‑ttacc                                                                                                                                        >  6:158047/1‑145 (MQ=255)    ccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGA‑ttacct                                                                                                                                       <  4:60371/149‑6 (MQ=255)         ccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGc                                                                                                                                    >  3:187374/1‑148 (MQ=255)         ccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGc                                                                                                                                    >  3:181452/1‑148 (MQ=255)         ccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGc                                                                                                                                    <  1:382391/148‑1 (MQ=255)          cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGa                                                                                                                                  >  1:261787/1‑149 (MQ=255)          cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGa                                                                                                                                  >  8:99008/1‑149 (MQ=255)          cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCAGATTACCTGTGCg                                                                                                                                   >  4:382519/1‑148 (MQ=255)           tCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGACTCGATCCTGATGGGCTACATCAACCACCTGCAGCGCGAGCGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCAGTGCGa                                                                                                                                  >  3:374758/1‑148 (MQ=255)             aaTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGATGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACAt                                                                                                                               <  4:100751/149‑1 (MQ=255)              aTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATg                                                                                                                              <  6:15273/149‑1 (MQ=255)              aTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATg                                                                                                                              <  2:195524/149‑1 (MQ=255)                  ccTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGcc                                                                                                                           >  8:251169/1‑148 (MQ=255)                  ccTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGA‑ttac                                                                                                                                         <  6:179961/133‑4 (MQ=255)                  ccTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGA‑ttac                                                                                                                                         >  5:179961/1‑130 (MQ=255)                    tACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAg                                                                                                                        >  6:39554/1‑149 (MQ=255)                         gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCt                                                                                                                   >  5:214182/1‑149 (MQ=255)                          tAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCGG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCt                                                                                                                   >  7:229257/1‑148 (MQ=255)                            gagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGATGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                                <  5:186964/149‑1 (MQ=255)                            gagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtga                                                                                                                 <  5:198494/148‑1 (MQ=255)                               cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGaa                                                                                                             >  8:202638/1‑149 (MQ=255)                                 cGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAAc                                                                                                            >  2:291549/1‑148 (MQ=255)                                        cccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGCAGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                      <  1:291549/149‑1 (MQ=255)                                        cccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCga                                                                                                    <  1:180525/149‑1 (MQ=255)                                               aCGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGGCATGGCCAAGGCGCTGATGAACGATATCGAGATCGc                                                                                              >  5:129456/1‑148 (MQ=255)                                                   ggCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAgg                                                                                         <  5:158047/149‑1 (MQ=255)                                                        aCGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAg                                                                                    >  8:218662/1‑149 (MQ=255)                                                            gCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgcg                                                                                 >  2:375726/1‑148 (MQ=255)                                                             cATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGTGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCa                                                                               >  3:225880/1‑149 (MQ=255)                                                              aTGTCTCGATCCTGATGTGCTACATCAAGCACCTGGTGCGCGAGGGCGATATTGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCaa                                                                                           <  3:221803/136‑1 (MQ=255)                                                              aTGTCTCGATCCTGATGGGCTACATCAAGAACCTGGTGCGCGAGTGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGAAGCCGATTAACTGTGAGACATGGCCAAGGCGATGATGAACGATATCTAGATCGCCaa                                                                                           >  4:221803/1‑136 (MQ=255)                                                               tGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAtt                                                                             <  7:251169/149‑1 (MQ=255)                                                                gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt                                                                              >  5:2255/1‑147 (MQ=255)                                                                 tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTa                                                                            <  6:2255/148‑1 (MQ=255)                                                                   tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGCTCGCCAAGGGTAAGGCGCATTAgg                                                                          >  6:384195/1‑148 (MQ=255)                                                                   tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAgg                                                                          >  1:263313/1‑148 (MQ=255)                                                                     gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGc                                                                         <  2:261787/147‑1 (MQ=255)                                                                      aTCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAtt                                                                      >  1:379494/1‑149 (MQ=255)                                                                            aTGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcg                                                                  <  2:106573/147‑1 (MQ=255)                                                                                 cTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAt                                                           >  7:360150/1‑149 (MQ=255)                                                                                    cATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTT‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAttt                                                         <  7:74852/148‑1 (MQ=255)                                                                                      tCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                      <  4:225880/149‑1 (MQ=255)                                                                                        aaGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                     <  7:99008/148‑1 (MQ=255)                                                                                             ccTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCgg                                               >  4:363891/1‑149 (MQ=255)                                                                                              cTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGc                                              >  3:33612/1‑149 (MQ=255)                                                                                                    cgcgAGGGCGATATGGAGGACGACCATAAATTGCTG‑‑GGGGCATCCGATTACCTGTGGGACATGGCCTAGGCGCTGATGAACGATATCGAGAGCGCCTTGGGTAAGGCGCATTAGGCTTTGACGCGGTCATTTGTGGGAGCGGCGTTGc                                         <  4:374758/148‑1 (MQ=255)                                                                                                    cgcgAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGc                                         <  3:382519/148‑1 (MQ=255)                                                                                                      cgAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGGCATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTc                                      <  6:129456/149‑1 (MQ=255)                                                                                                         gggCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTcgc                                    <  8:360150/148‑1 (MQ=255)                                                                                                            cGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAt                                <  7:218662/149‑1 (MQ=255)                                                                                                            cGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAt                                <  1:375726/149‑1 (MQ=255)                                                                                                               tatGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATggg                             <  4:33612/149‑1 (MQ=255)                                                                                                                 tGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGc                            <  4:187374/148‑1 (MQ=255)                                                                                                                 tGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGc                            <  4:181452/148‑1 (MQ=255)                                                                                                                 tGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGc                            <  4:255799/148‑1 (MQ=255)                                                                                                                       acgacCATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGCTATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAag                     <  8:229257/149‑1 (MQ=255)                                                                                                                            cATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCtt                                           >  6:43293/1‑122 (MQ=255)                                                                                                                            cATAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCtt                                           <  5:43293/122‑1 (MQ=255)                                                                                                                              tAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                      <  6:80028/109‑1 (MQ=255)                                                                                                                              tAAATTCCTG‑‑GGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGcccc              >  5:124242/1‑149 (MQ=255)                                                                                                                                            ggCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGGCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCaga  <  3:363891/149‑1 (MQ=255)                                                                                                                                             gCAGCCGATTACCTGTCCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCa    >  2:216599/1‑146 (MQ=255)                                                                                                                                                     |                                                                                                                                            AACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTG‑‑GGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335979‑336263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑