Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,027,284:1	+C	7.6%	coding (594/993 nt)	ocd →	ornithine cyclodeaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,027,284	1	.	C	7.6%	256.9 / 11.0	66	coding (594/993 nt)	ocd	ornithine cyclodeaminase
Reads supporting (aligned to +/- strand):  ref base . (33/28);  new base C (2/3);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCGCTCCTTCTTGTGCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGAGGTAATAGCAGCAGTCGATCACT  >  NC_002947/5027136‑5027429                                                                                                                                                      |                                                                                                                                                  aaGCGCTCCTTCTTGTGCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCt                                                                                                                                                    <  8:261205/149‑1 (MQ=255)       tCCTTCTTGTGCGGGATATCATGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACAtt                                                                                                                                             >  3:219179/1‑149 (MQ=255)         cttcttGTGCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTg                                                                                                                                            <  2:113580/148‑1 (MQ=255)         cttcttGTGCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTg                                                                                                                                            <  4:257026/148‑1 (MQ=255)         cttcttGTGCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTg                                                                                                                                            <  1:351645/148‑1 (MQ=255)          ttcttGTGCGGGATATCAAGCGAGTTCGTGTATTGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTATACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTC‑AGCGCTCGACATTGct                                                                                                                                          <  2:114302/149‑2 (MQ=255)          ttcttGTGCGGGATATCAAGCGAGTTAGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGACTTGCAGCTATCGAGACTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTC‑AGCGCTCGACATTGct                                                                                                                                          >  1:114302/1‑148 (MQ=255)            cttGTGCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCa                                                                                                                                          <  1:141303/147‑1 (MQ=255)            cttGTGCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTC‑AGCGCTCGACATTGct                                                                                                                                          <  6:370978/147‑2 (MQ=255)             ttGTGCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGt                                                                                                                                        >  2:182525/1‑148 (MQ=255)               gtgCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTC‑AGCGCTCGACATTGcttc                                                                                                                                        <  1:258500/146‑4 (MQ=255)                tgCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCAcc                                                                                                                                    >  8:106708/1‑149 (MQ=255)                tgCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCAc                                                                                                                                     <  6:124606/148‑1 (MQ=255)                 gCGGTATATCAAGCGAGTTCGGGTATGGGCTAGTAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCt                                                                                                                                   <  6:282277/149‑1 (MQ=255)                           atgCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAACGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAg                                                                                                                         <  4:219179/147‑1 (MQ=255)                           aaGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCaa                                                                                                                          <  7:106708/148‑1 (MQ=255)                            aGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGc                                                                                                                        <  1:182525/149‑1 (MQ=255)                            aGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTAACCTGCACGCCAAg                                                                                                                         >  8:83122/1‑148 (MQ=255)                                aGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATc                                                                                                                    <  8:113158/149‑1 (MQ=255)                                  ttCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCaa                                                                                                                  >  4:160773/1‑149 (MQ=255)                                    cGGGTATGGTCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCGTTGCAGCTATCTAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAAc                                                                                                                 <  3:233111/148‑1 (MQ=255)                                     gggTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAAcc                                                                                                                >  3:35416/1‑148 (MQ=255)                                                 ggAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCAACGGCCTGGAAGCCAATGACGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAACCAATCAACAAATTTTACATCGc                                                                                                   >  5:214618/1‑149 (MQ=255)                                                  gAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCa                                                                                                  <  2:53440/149‑1 (MQ=255)                                                     ttCCGCAAAAGCGCAGCAACTGGCCTTGCATCTAGCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACTCCAAGCAATCAACCAATTTTACATCGCAgg                                                                                                <  6:63724/148‑1 (MQ=255)                                                     ttCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATc                                                                                                                    >  5:220771/1‑128 (MQ=255)                                                     ttCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATc                                                                                                                    <  6:220771/128‑1 (MQ=255)                                                        cGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCaa                                                                                                                      <  8:107974/123‑1 (MQ=255)                                                        cGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCaa                                                                                                                      >  7:107974/1‑123 (MQ=255)                                                        cGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGACACTGCCGCAGAGTCGGTAGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACt                                                                                            >  3:262989/1‑149 (MQ=255)                                                          cAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGc                                                                                               <  4:255299/144‑1 (MQ=255)                                                          cAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGc                                                                                               >  3:255299/1‑144 (MQ=255)                                                          cAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTg                                                                                          >  6:142975/1‑149 (MQ=255)                                                               gcgcAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGcc                                                                                     <  4:106254/149‑1 (MQ=255)                                                                 gcagcaACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCg                                                                                   <  8:112882/149‑1 (MQ=255)                                                                 gcagcaACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCg                                                                                   >  7:272191/1‑149 (MQ=255)                                                                 gcagcaACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCg                                                                                   <  8:220501/149‑1 (MQ=255)                                                                   agcaACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTAGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCgg                                                                                  >  7:333795/1‑148 (MQ=255)                                                                        cTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCa                                                                            >  1:88591/1‑149 (MQ=255)                                                                           gCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCatat                                                                         <  4:35416/149‑1 (MQ=255)                                                                                gCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGTAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCTTATCACgg                                                                    >  2:300448/1‑149 (MQ=255)                                                                                 cAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCAAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATTTCACGGc                                                                   >  1:110659/1‑149 (MQ=255)                                                                                   gCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTa                                                                 >  4:170820/1‑149 (MQ=255)                                                                                    cTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTAt                                                                >  8:357364/1‑149 (MQ=255)                                                                                       tCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTAgggg                                                             >  4:197942/1‑149 (MQ=255)                                                                                         gAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCa                                                                                                  >  1:164644/1‑110 (MQ=255)                                                                                         gAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCa                                                                                                  <  2:164644/110‑1 (MQ=255)                                                                                                  cGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GCCATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACt                                                   >  6:273119/1‑148 (MQ=255)                                                                                                     ccTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACGAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGaa                                                >  4:61842/1‑148 (MQ=255)                                                                                                       tGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCCAGCGCT‑GACATTGAAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAaca                                              >  4:15405/1‑148 (MQ=255)                                                                                                           aGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACaaa                                          >  4:317173/1‑148 (MQ=255)                                                                                                                cTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAg                                    <  3:170820/149‑1 (MQ=255)                                                                                                                       agagTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTc                             <  3:160773/149‑1 (MQ=255)                                                                                                                         agTCGGTCGATGAAGCAAAGTCGAGCTCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTAGGGGGGCAGACACTGAACACAAAAACGAGGttcaggct                           >  8:149722/1‑143 (MQ=255)                                                                                                                               tcgATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGa                         <  2:69885/145‑1 (MQ=255)                                                                                                                               tcgATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGa                         >  1:69885/1‑145 (MQ=255)                                                                                                                                 gATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTTCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTATCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGAGGTAAt                   <  4:262989/149‑1 (MQ=255)                                                                                                                                   tGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGa                                                 <  5:150146/117‑1 (MQ=255)                                                                                                                                   tGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGa                                                 >  6:150146/1‑117 (MQ=255)                                                                                                                                   tGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGAGGTAATag                 >  4:374034/1‑149 (MQ=255)                                                                                                                                      tgCAATGTC‑AGCGCTCGACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGAGGTAATagcag              >  7:300210/2‑149 (MQ=255)                                                                                                                                       gCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGAGGTAATagcag              >  4:96608/1‑148 (MQ=255)                                                                                                                                        cAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGAGGTAATagcag              >  7:64101/1‑147 (MQ=255)                                                                                                                                          aTGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACAGAACACAAAAACGAGGTTGCTCCTGAGGTAATAGCAGCAGt          >  5:327803/1‑149 (MQ=255)                                                                                                                                              cGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGAGGTAATAGCAGCAGTCGAt      >  4:279199/1‑149 (MQ=255)                                                                                                                                                aGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAAATTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGAGGTAATAGCAGCAGTCGATCa    <  7:83122/149‑1 (MQ=255)                                                                                                                                                  cgcT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGAGGTAATAGCAGCAGTCGATCACt  <  3:317173/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  AAGCGCTCCTTCTTGTGCGGGATATCAAGCGAGTTCGGGTATGGGCTAGGAGTTCCGCAAAAGCGCAGCAACTGGCCTTGCAGCTATCGAGCCTCTACGGCCTGGAAGCCACTGCCGCAGAGTCGGTCGATGAAGCAATGTCGAGCGCT‑GACATTGCAGTCACCTGCACGCCAAGCAATCAACCAATTTTACATCGCAGGCACTTGCGGCCCGGTCTCCATATCACGGCTATGGGGGCAGACACTGAACACAAAAACGAGGTTGCTCCTGAGGTAATAGCAGCAGTCGATCACT  >  NC_002947/5027136‑5027429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑