Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,089,078:1 +C 5.3% coding (761/1029 nt) astA‑I ← arginine N‑succinyltransferase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,089,0781.C 5.3% 306.7 / 11.1 76coding (761/1029 nt)astA‑Iarginine N‑succinyltransferase subunit beta
Reads supporting (aligned to +/- strand):  ref base . (36/36);  new base C (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

AGTGATGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACAGCGGGAACTTGGGCA  >  NC_002947/5088936‑5089222
                                                                                                                                               |                                                                                                                                                 
aGTGATGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCg                                                                                                                                              >  7:6823/1‑148 (MQ=255)
 gTGATGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGaa                                                                                                                                            <  6:166161/149‑1 (MQ=255)
 gTGATGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGCCCGGCCATCGa                                                                                                                                             <  3:347807/149‑1 (MQ=255)
 gTGATGCGACACTCCTCGCGATTGCGGTTGTGGAGGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGAT‑GCCGGCCCGGCCATCGag                                                                                                                                            <  4:224657/149‑2 (MQ=255)
    aTGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGa                                                                                                                                          <  4:322377/148‑1 (MQ=255)
    aTGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGa                                                                                                                                          <  6:21714/148‑1 (MQ=255)
    aTGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGAt                                                                                                                                         <  3:294484/149‑1 (MQ=255)
        gACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTc                                                                                                                                      >  1:68242/1‑148 (MQ=255)
               gtcgCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGc                                                                                                                              >  6:391196/1‑149 (MQ=255)
                tcgCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGcc                                                                                                                             >  6:90670/1‑149 (MQ=255)
                tcgCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGcc                                                                                                                             >  6:302106/1‑149 (MQ=255)
                  gcgcTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGccc                                                                                                                            <  2:68242/148‑1 (MQ=255)
                  gcgcTTGCGGGTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCt                                                                                                                           <  8:240054/149‑1 (MQ=255)
                    gcTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑gg                                                                                                                        >  7:78344/1‑149 (MQ=255)
                          ggTTGTGGATTATGAAAGGGGTCGCGTCATCGCCTGTCGTAGCTACCGCCAGCACCAGGGTCTGGCTGTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGtt                                                                                                                   <  8:6823/148‑1 (MQ=255)
                          ggTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTg                                                                                                                  <  1:13997/149‑1 (MQ=255)
                            ttGTGGATGATGAAAGAGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGaa                                                                                                                <  7:284447/149‑1 (MQ=255)
                                 gatgatGACAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCtt                                                                                                           <  8:75550/149‑1 (MQ=255)
                                  atgatgAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTc                                                                                                          <  2:28651/149‑1 (MQ=255)
                                       gaAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAAGGTCTGGCTCTAGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCt                                                                                                     >  1:79395/1‑149 (MQ=255)
                                        aaaGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCtt                                                                                                    <  5:391196/149‑1 (MQ=255)
                                        aaaGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCt                                                                                                     <  1:195252/148‑1 (MQ=255)
                                          aGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCtt                                                                                                    >  2:367187/1‑147 (MQ=255)
                                                  cgTCNTCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGc                                                                                           >  5:26934/1‑148 (MQ=255)
                                                   gTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGt                                                                                                                                       <  4:34040/104‑1 (MQ=255)
                                                   gTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGt                                                                                                                                       >  3:34040/1‑104 (MQ=255)
                                                    tCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAg                                                                                        >  5:289279/1‑149 (MQ=255)
                                                     cATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAgc                                                                                       >  8:87520/1‑149 (MQ=255)
                                                      aTCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAgcg                                                                                      >  6:247295/1‑149 (MQ=255)
                                                       tCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAgcgc                                                                                     >  8:67916/1‑149 (MQ=255)
                                                         gCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAg                                                                                                                     >  7:121272/1‑115 (MQ=255)
                                                         gCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAg                                                                                                                     <  8:121272/115‑1 (MQ=255)
                                                         gCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGcc                                                                                    <  8:78344/148‑1 (MQ=255)
                                                         gCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCg                                                                                   >  8:228206/1‑149 (MQ=255)
                                                            ttgCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCt                                                                                >  7:249096/3‑149 (MQ=255)
                                                              gCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCg                                                                              <  2:66256/149‑1 (MQ=255)
                                                                gTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGgt                                                                            <  8:222845/149‑1 (MQ=255)
                                                                gTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGgt                                                                            <  2:336677/149‑1 (MQ=255)
                                                                  acctaccGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGgtgt                                                                          >  2:223118/1‑149 (MQ=255)
                                                                    ctaccGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑gg                                                                                                                        <  3:197420/101‑1 (MQ=255)
                                                                    ctaccGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCt                                                                        <  7:67916/149‑1 (MQ=255)
                                                                    ctaccGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCt                                                                        >  6:13996/1‑149 (MQ=255)
                                                                    ctaccGCCAGCACCAGGGTCTGGCTCGCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑gg                                                                                                                        >  4:197420/1‑101 (MQ=255)
                                                                      accGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCttt                                                                      >  2:247234/1‑149 (MQ=255)
                                                                        cGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgt                                                                    <  2:353888/149‑1 (MQ=255)
                                                                          ccAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgtg                                                                   >  1:214059/1‑148 (MQ=255)
                                                                           cAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGca                                                                 <  2:214059/149‑1 (MQ=255)
                                                                                ccAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgt                                                                    <  1:255017/141‑1 (MQ=255)
                                                                                ccAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgt                                                                    >  2:255017/1‑141 (MQ=255)
                                                                                 cAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGc                                                           >  2:150176/1‑149 (MQ=255)
                                                                                 cAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACCTAGCCCT‑GGTAGTTGAAGCCTTACTGCTTGAGCATGGCCAGCGCCGGCTAGGTGTCTTTGTGCACACGGc                                                           >  3:216433/1‑149 (MQ=255)
                                                                                     gTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGAt                                                       <  5:13996/149‑1 (MQ=255)
                                                                                      tCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATc                                                      <  6:26934/149‑1 (MQ=255)
                                                                                          gCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgt                                                                    <  3:313443/131‑1 (MQ=255)
                                                                                          gCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTtgt                                                                    >  4:313443/1‑131 (MQ=255)
                                                                                          gCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCacac                                                  >  1:175773/1‑149 (MQ=255)
                                                                                           ctctCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCg                                                                              <  6:162537/120‑1 (MQ=255)
                                                                                           ctctCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCg                                                                              >  5:162537/1‑120 (MQ=255)
                                                                                           ctctCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACt                                                 <  1:150176/149‑1 (MQ=255)
                                                                                            tctcGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGa                                                                                                  >  3:344232/1‑99 (MQ=255)
                                                                                            tctcGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGa                                                                                                  <  4:344232/99‑1 (MQ=255)
                                                                                                gcgTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTgcgcg                                            >  3:175723/1‑149 (MQ=255)
                                                                                                      ggCGCGGATCTTGGCAGTGTCACACTCGATGGCCGAGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCtt                                      >  1:57660/1‑149 (MQ=255)
                                                                                                         gcgGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCg                                    >  3:176794/1‑148 (MQ=255)
                                                                                                          cgGATCTTGGCAGTGTCACACTCCATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGa                                  <  6:171135/149‑1 (MQ=255)
                                                                                                            gATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACa                                <  1:223118/149‑1 (MQ=255)
                                                                                                            gATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACa                                <  2:79395/149‑1 (MQ=255)
                                                                                                               cTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAgg                              >  7:148928/1‑148 (MQ=255)
                                                                                                                     aGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAgg                       >  1:266410/1‑149 (MQ=255)
                                                                                                                       tgtCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGgtg                     <  4:60544/149‑1 (MQ=255)
                                                                                                                         tCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGgtgt                    <  5:247295/148‑1 (MQ=255)
                                                                                                                         tCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTa                   <  1:367187/149‑1 (MQ=255)
                                                                                                                           atcttCGAT‑GCCGGCCCGGCCATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTa                   >  5:185310/5‑147 (MQ=255)
                                                                                                                           acacTCGATGGCCGGTCCGG‑CATCGAAGATGTCGACGTAGACCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACa                 >  7:107061/1‑149 (MQ=255)
                                                                                                                           acacTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTa                   >  3:158354/1‑147 (MQ=255)
                                                                                                                            tcttCGAT‑GCCGGCCCGGCCATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTTTACAg                >  3:56265/4‑149 (MQ=255)
                                                                                                                                      cctgcccgg‑CATCGAAGATGTCGAAGTAGCCAT‑GGAAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCCCCGGCTCGGTGTCTTAGTGCACACCGCCGATCACACTGCGCGCCGCTTCGCACAGGAAGCAGGAGTACAGCGGGAACATg      >  8:360518/6‑149 (MQ=255)
                                                                                                                                      ccgggccgg‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACAGCGGGAACTTg      >  6:223665/1‑149 (MQ=255)
                                                                                                                                      ccgggccgg‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACAGCGGGAACTTg      <  2:266410/149‑1 (MQ=255)
                                                                                                                                       cgggccgg‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACAGCGGGAACTTgg     <  7:87520/149‑1 (MQ=255)
                                                                                                                                            cgg‑CATCGAAGATGTCGACGTAGCCCTGGGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACAGCGGGAACTTGGGCa  >  1:23659/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
AGTGATGCGACAGTCGTCGCGCTTGCGGTTGTGGATGATGAAAGGGGTCGCGTCATCGCCTGGCGTACCTACCGCCAGCACCAGGGTCTGGCTCTCGCGTACGGCGCGGATCTTGGCAGTGTCACACTCGATGGCCGGGCCGG‑CATCGAAGATGTCGACGTAGCCCT‑GGTAGTTGAAGCCTTCCTGCTTGAGCATGGCCAGCGCCGGCTCGGTGTCTTTGTGCACACGGCCGATCACACTGCGCGCCGCTTCGGACAGGAAGCAGGTGTACAGCGGGAACTTGGGCA  >  NC_002947/5088936‑5089222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑