| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,430,397:1 | +A | 9.4% | intergenic (‑79/+82) | PP_4769 ← / ← PP_4770 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,430,397 | 1 | . | A | 9.4% | 201.3 / 16.5 | 53 | intergenic (‑79/+82) | PP_4769/PP_4770 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (21/27); new base A (3/2); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01 | |||||||||||
GGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTAT > NC_002947/5430249‑5430545 | ggCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAt > 7:252116/1‑149 (MQ=255) ggtccTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCTCGA‑TCTTTTTGACCTGGGAACCTCATTTTTTGCCTGGAGTGCCGTTGGGCTGCTGCTCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGt < 4:248543/144‑1 (MQ=255) cgcACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg < 1:87880/149‑1 (MQ=255) cgaaCGGTGTAGTAGAGGTTCTGCAAATCGGCGAACAGCGCGA‑TCTTTTTCCACTGGGCACCTCATTTTTGGCCTGGAGTGCC‑GGTGGC‑GCTTTGCGCCCCTTCGCGGGCACGCCCGCTCCCAAGGGTGTACGCGCG‑ATACCTGTGGGa < 7:48617/146‑1 (MQ=9) gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCgg < 7:256116/149‑1 (MQ=255) tagtagAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGc < 8:39489/148‑1 (MQ=255) gtagAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAg < 4:105916/141‑1 (MQ=37) gtagAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAg > 3:105916/1‑141 (MQ=37) gtagAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTg > 3:266625/1‑149 (MQ=255) tagAGGTTCTGCACATCGGCGAACAGCGCGATTCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTg > 1:46479/1‑149 (MQ=255) tagAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGc < 1:139047/149‑1 (MQ=255) gCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAggg > 5:117929/1‑149 (MQ=255) cacaTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAgggg < 3:351767/149‑1 (MQ=255) cacaTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAg < 5:388607/146‑1 (MQ=255) cacaTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCGGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAggg < 4:222475/148‑1 (MQ=255) acaTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGgc < 3:156162/149‑1 (MQ=255) caTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAg > 1:110533/1‑144 (MQ=255) caTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAg < 2:110533/144‑1 (MQ=255) caTCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCCTCGCGGGCACCCCCCCGCCCCCCGGGAT‑AGCGGGGGACCCCGGGGGCGGGGGGGTGCCC‑GCGCAgggggg > 6:207669/1‑147 (MQ=255) ggCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCaaa < 2:88287/149‑1 (MQ=255) gCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAg > 7:54752/1‑149 (MQ=255) attttttcCCCCGGGGACCCCCATTTTGCGCCTGGATGGCCGGGGCGCGGCGCCCCCGACCTCCGCGGACACCCCCGCTCCACCCCGTCT‑GGCGGGTACCCCGGTGGGTGGGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGaaa < 4:198764/146‑1 (MQ=255) tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGTTAGTGGGCGTGCCCTGCGAAGGGGCGCAAAGAGCCCCCTGAAATGc < 1:41680/148‑1 (MQ=255) tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGc > 3:207303/1‑147 (MQ=255) tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCCTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAAt < 5:207669/145‑1 (MQ=255) ttCTCCTGGGAAGCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCca < 4:356660/148‑1 (MQ=255) cTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTa > 1:96488/1‑149 (MQ=255) ggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCTCAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCG‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTAt < 3:34054/147‑1 (MQ=255) aaCCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCa < 4:266625/149‑1 (MQ=255) cTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGCAGGGGCGCAACGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc > 8:154969/1‑149 (MQ=255) cTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc < 5:113980/149‑1 (MQ=255) cTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACACCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc > 3:135008/1‑149 (MQ=255) cTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCGGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc > 6:370457/1‑149 (MQ=255) tCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAcc > 5:163332/1‑149 (MQ=255) tCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCCCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAc > 1:115476/1‑148 (MQ=255) tttttGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGt < 2:238120/149‑1 (MQ=255) tgtccTGGAGTGCCGGGGGGCGGCTGCGCAGACCTTCGCGGACACGGCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAg < 6:230030/145‑1 (MQ=255) ggCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGa < 4:207303/148‑1 (MQ=255) ccTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACg > 5:367045/1‑148 (MQ=255) cGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc < 3:79088/149‑1 (MQ=255) cGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc < 8:252116/149‑1 (MQ=255) cTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCga < 4:146850/135‑1 (MQ=255) cTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCga > 3:146850/1‑135 (MQ=255) cgGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGGATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCg > 5:190469/1‑149 (MQ=255) acacGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCTGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTg > 8:103695/1‑149 (MQ=255) cacGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGc < 8:54752/149‑1 (MQ=255) acGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGc > 3:370577/1‑148 (MQ=37) ccGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGCCC‑GCGACGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaaa > 7:248244/1‑149 (MQ=37) ccACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCg > 5:73562/1‑149 (MQ=255) acaGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTc > 7:110340/1‑149 (MQ=255) aGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCgg > 2:161321/1‑149 (MQ=255) ggTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGc < 6:367045/149‑1 (MQ=255) ggTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGc < 2:115476/149‑1 (MQ=255) aT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTa > 2:244171/1‑149 (MQ=255) t‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCGCCGTCGGCGTAt > 6:97578/1‑149 (MQ=255) t‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTAt > 2:138079/1‑149 (MQ=255) | GGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGA‑TCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCC‑GCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTAT > NC_002947/5430249‑5430545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |