| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,070,246:1 | +GA | 100% | intergenic (+46/+96) | aroP‑I → / ← PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,070,246 | 1 | . | G | 86.7% | 127.5 / 15.6 | 45 | intergenic (+46/+96) | aroP‑I/PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Reads supporting (aligned to +/- strand): ref base . (0/6); new base G (18/21); total (18/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,070,246 | 2 | . | A | 86.7% | 119.8 / 15.8 | 45 | intergenic (+46/+96) | aroP‑I/PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Reads supporting (aligned to +/- strand): ref base . (0/6); new base A (18/21); total (18/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTAC > NC_002947/1070100‑1070378 || tGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCt‑‑gt > 6:118282/1‑147 (MQ=255) aCGGCGCCGATTCGCATCTCTGTTATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑Ga < 7:299402/147‑1 (MQ=255) aCGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCt‑‑gt > 5:361793/1‑145 (MQ=255) aCGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCt‑‑gt < 6:361793/145‑1 (MQ=255) aCGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑Ga < 4:154174/147‑1 (MQ=255) gccgccGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCTTTAGGCTTTCATCGCCt‑‑gt > 1:240014/1‑143 (MQ=255) cgccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑Gagatcga < 5:235533/149‑7 (MQ=255) cgccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑Gagatcga < 5:156864/149‑7 (MQ=255) gccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑Gagatcgag < 2:201679/149‑8 (MQ=255) ttCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGACAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGcgccg > 3:342999/1‑149 (MQ=255) gCATCTCGGT‑ATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCcgcgcg > 2:120837/1‑149 (MQ=255) cATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCcgcgcg < 7:6300/149‑1 (MQ=255) ctcGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTGTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCgcggg > 1:85633/1‑149 (MQ=255) ctcGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTA‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCgcgggc > 5:269779/1‑149 (MQ=255) ggTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCgcggg < 7:52790/145‑1 (MQ=255) aaTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGACGCGCGGGCGGCGCt > 4:341747/1‑149 (MQ=255) aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCTTTAGGCTTTCATCGCCT‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCt < 3:289936/148‑1 (MQ=37) tGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCTCg < 6:35883/149‑1 (MQ=38) cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGTGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAt < 6:225152/149‑1 (MQ=38) cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCTTTAGGCTTTCATCGCCT‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCTCGa < 2:240014/148‑1 (MQ=25) cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAt > 5:225152/1‑149 (MQ=38) aTCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtct < 5:118282/148‑1 (MQ=255) ggCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAgg < 3:341747/149‑1 (MQ=255) tGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGGGA‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGAt < 8:359441/149‑1 (MQ=255) ggATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGAt < 4:342999/148‑1 (MQ=255) ggtgtgctgtgGGTGGCTT‑TCATGCTTAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGc < 2:382338/147‑1 (MQ=38) tgctgtgGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTGGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCt < 1:120837/147‑1 (MQ=255) gtgggtggCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCgcggg > 8:145075/1‑112 (MQ=255) ggtggCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATg < 1:241300/149‑1 (MQ=255) gCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTACGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGACGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTc < 4:246354/149‑1 (MQ=38) tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGCTCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTggc > 4:212940/1‑149 (MQ=255) tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTggc > 4:140349/1‑149 (MQ=255) tGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCt > 5:349853/1‑149 (MQ=255) tGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGCTTTCATCGCCT‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCt < 2:262359/102‑1 (MQ=18) tGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGCTTTCATCGCCT‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCt > 1:262359/1‑102 (MQ=18) agaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGAc < 3:140349/149‑1 (MQ=255) gCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGtt > 5:171662/1‑149 (MQ=255) tCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCTc < 1:173075/93‑1 (MQ=25) tCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCTc > 2:173075/1‑93 (MQ=25) cAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCtt > 6:85975/1‑149 (MQ=255) ccAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGc < 4:343187/106‑1 (MQ=255) ccAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGc > 3:343187/1‑106 (MQ=255) cAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATg < 2:85633/149‑1 (MQ=255) aaGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAg > 1:56303/1‑149 (MQ=255) aGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAg < 6:269779/148‑1 (MQ=255) gTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtgg > 6:325707/1‑149 (MQ=255) ggTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTa < 2:56303/149‑1 (MQ=255) ggCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTAc > 7:43961/1‑143 (MQ=255) ggCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTAc < 8:43961/143‑1 (MQ=255) || TGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTT‑TCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTAC > NC_002947/1070100‑1070378 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |