Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,463,417:1	+A	5.5%	coding (765/1116 nt)	algL ←	alginate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,463,417	1	.	A	5.5%	297.1 / 11.4	73	coding (765/1116 nt)	algL	alginate lyase
Reads supporting (aligned to +/- strand):  ref base . (35/34);  new base A (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

GTGGCTGGGGTCGCGGGCGCCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTAGCCATGACCGACCAGGCCGC  >  NC_002947/1463270‑1463560                                                                                                                                                     |                                                                                                                                                gTGGCTGGGGTCGCGGGCGCCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAgc‑g                                                                                                                                                >  6:246836/1‑149 (MQ=255)  tGGCTGGGGTCGCGGGCGCCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAgc‑gc                                                                                                                                               <  3:285849/149‑1 (MQ=255)    gCTGGGGTCGCGGGCGCCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCACGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAACGCGTAGTTGAGATAGGCCAga‑gcgc                                                                                                                                             >  4:336486/1‑149 (MQ=255)        ggggCGCGGGCGCCGGCCTGCAGGCCTTCAGCCAGGCGCTGCAAGGCGTAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGTGCGAAGCTGGCATTCATCGGCAGCGGCGGCAAGTCGTAGTTGTGATAGGCCAGC‑GCGCgct                                                                                                                                          <  8:259787/148‑1 (MQ=255)        gggTCGCGGGCGCCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCgct                                                                                                                                          <  5:246836/148‑1 (MQ=255)           tCGCGGGCGCCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGctgctg                                                                                                                                      >  5:343845/1‑149 (MQ=255)           tCGCGGGCGCCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGctgctg                                                                                                                                      >  7:220509/1‑149 (MQ=255)              cgGGCGCCGGCCAGCACGCCTTCACACAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGGGATAGGCCAGC‑GCGCGCTGCTGgcg                                                                                                                                   >  8:362235/1‑149 (MQ=255)               gggCGCCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCAGCGCGC‑GCTGgcct                                                                                                                                  <  4:247405/149‑3 (MQ=255)                  cgcCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCAGCGCGC‑GCTGgcctatc                                                                                                                               <  8:94165/149‑6 (MQ=255)                  cgcCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGATGGCGCTTg                                                                                                                               >  5:260997/1‑149 (MQ=255)                   gcCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGCAGCGCGC‑GCTGgcctatca                                                                                                                              >  6:45524/1‑143 (MQ=255)                   gcCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGa                                                                                                                              <  1:184358/149‑1 (MQ=255)                     cGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGAtt                                                                                                                            >  8:27065/1‑149 (MQ=255)                         cAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGt                                                                                                                        <  5:283618/149‑1 (MQ=255)                            cACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTgg                                                                                                                     >  2:133474/1‑149 (MQ=255)                             aCGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTggg                                                                                                                    <  7:220450/149‑1 (MQ=255)                               gCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCg                                                                                                                         >  8:184913/1‑142 (MQ=255)                               gCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCg                                                                                                                         <  7:184913/142‑1 (MQ=255)                               gCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTggg                                                                                                                    >  4:260304/1‑147 (MQ=255)                               gCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCa                                                                                                                  <  8:220509/149‑1 (MQ=255)                                    cACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAg                                                                                                             >  3:108915/1‑149 (MQ=255)                                       ccAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGccc                                                                                                          <  8:191996/149‑1 (MQ=255)                                       ccAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGccc                                                                                                          >  3:266726/1‑149 (MQ=255)                                       ccAGGCGCAGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGccc                                                                                                          >  5:82119/1‑149 (MQ=255)                                         aGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTg                                                                                                        <  1:33534/149‑1 (MQ=255)                                         aGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTg                                                                                                        <  3:179818/149‑1 (MQ=255)                                          ggCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTgg                                                                                                       <  6:55206/149‑1 (MQ=255)                                          ggCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTgg                                                                                                       <  6:190086/149‑1 (MQ=255)                                          ggCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTgg                                                                                                       <  5:126228/149‑1 (MQ=255)                                             gcTGCAAGGCGAAGTTGTTCTCGGCGAGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCa                                                                                                    <  5:69239/149‑1 (MQ=255)                                                 cAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATcc                                                                                                 <  7:312533/148‑1 (MQ=255)                                                   aGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCAcc                                                                                              >  5:214413/1‑149 (MQ=255)                                                   aGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCAcc                                                                                              <  6:343845/149‑1 (MQ=255)                                                   aGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCAcc                                                                                              <  1:261731/149‑1 (MQ=255)                                                   aGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCAGCAAGGCGAAGTTGTGATAGGCCAGC‑GCGCTCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCCTCCAcc                                                                                              >  7:258310/1‑149 (MQ=255)                                                         aGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTg                                                                                        >  4:38000/1‑149 (MQ=255)                                                              ttCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGcc                                                                                   <  3:260304/149‑1 (MQ=255)                                                                ctcGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGa                                                                                 >  3:128821/1‑149 (MQ=255)                                                                 tcGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGAc                                                                                >  6:314156/1‑149 (MQ=255)                                                                   ggCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGa                                                                                 <  7:33223/146‑1 (MQ=255)                                                                   ggCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGAcc                                                                               <  4:266726/148‑1 (MQ=255)                                                                          aGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGATTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTc                                                                       <  2:266417/149‑1 (MQ=255)                                                                            gTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCt                                                                     >  1:233513/1‑149 (MQ=255)                                                                            aactacgccttgccgccgctggcgatgattgccagcttcgcccaggccaatggcgtAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTAGTATTCCt                                                                     >  7:210583‑M2/57‑149 (MQ=255)                                                                              ctacgccttgccgccgctggcgatgattgccagcttcgcccaggccaatggcgtAGTTGTGTTAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTgg                                                                                                       <  5:275836‑M2/59‑1 (MQ=255)                                                                              ctacgccttgccgccgctggcgatgattgccagcttcgcccaggccaatggcgtAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTgg                                                                                                       >  6:275836‑M2/55‑113 (MQ=255)                                                                                    CATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGcc                                                             >  5:314156‑M1/1‑149 (MQ=255)                                                                                             gggCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATc                                                                                                  >  6:210268/1‑103 (MQ=255)                                                                                             gggCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATc                                                                                                  <  5:210268/103‑1 (MQ=255)                                                                                              ggCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGaa                                                     >  8:107119/1‑147 (MQ=255)                                                                                              ggCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAAc                                                    <  7:27065/148‑1 (MQ=255)                                                                                                cGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAgg                                                 >  6:195660/1‑149 (MQ=255)                                                                                                cGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAgg                                                 >  7:77225/1‑149 (MQ=255)                                                                                                    gCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTcgc                                             >  7:336955/1‑149 (MQ=255)                                                                                                       ggCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGAc                                          >  3:184180/1‑149 (MQ=255)                                                                                                       ggCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGAc                                          >  1:347596/1‑149 (MQ=255)                                                                                                        gCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGa                                           <  1:87348/147‑1 (MQ=255)                                                                                                        gCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGAcg                                         >  3:43074/1‑149 (MQ=255)                                                                                                          aaTCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGAcggt                                       <  4:128821/149‑1 (MQ=255)                                                                                                          aaTCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGAcggt                                       <  1:133474/149‑1 (MQ=255)                                                                                                                    aGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGGCGCGACGGTCGGTGGCGAc                             >  6:270098/1‑149 (MQ=255)                                                                                                                      cggcggCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGAcgg                           <  7:107119/149‑1 (MQ=255)                                                                                                                        gcggcAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATc                                                                                                  <  1:165748/76‑1 (MQ=255)                                                                                                                        gcggcAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATc                                                                                                  >  2:165748/1‑76 (MQ=255)                                                                                                                         cggcAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGAcggcgg                        <  5:270098/149‑1 (MQ=255)                                                                                                                           gcAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTa                      <  4:43074/149‑1 (MQ=255)                                                                                                                             aaGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTAGc                    >  8:356394/1‑149 (MQ=255)                                                                                                                             aaGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTAGc                    >  7:143416/1‑149 (MQ=255)                                                                                                                                gcgtAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTAGCCAt                 <  7:362235‑M2/145‑1 (MQ=255)                                                                                                                                  gtAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTAGCCATGa               >  2:346217‑M2/3‑149 (MQ=255)                                                                                                                                        gtgATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTAGCCATgaccgac          <  2:347596/148‑1 (MQ=255)                                                                                                                                          gATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTAGcc                   >  5:323408/1‑137 (MQ=255)                                                                                                                                          gATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTAGcc                   <  6:323408/137‑1 (MQ=255)                                                                                                                                             gcgCCAGCAGCGCGC‑GCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTAGCCATGACCGACCAGgcc    >  3:149091/3‑149 (MQ=255)                                                                                                                                               gCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTAGCCATGACCGACCAGgccgc  >  1:129647/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                GTGGCTGGGGTCGCGGGCGCCGGCCAGCACGCCTTCACCCAGGCGCTGCAAGGCGAAGTTGTTCTCGGCGCGCAGGTCGACGCCATTGGCCTGGGCGAAGCTGGCAATCATCGCCAGCGGCGGCAAGGCGTAGTTGTGATAGGCCAGC‑GCGCGCTGCTGGCGCTTGATTTCGTTGGGCAGGAAGCCCTGGTCATCCACCTGGTTGGCGCCGACCTTGTATTCCTTCACGGCCCAGTCGAACAGGTCGCGACGGTCGGTGGCGACGGCGGTAGCCATGACCGACCAGGCCGC  >  NC_002947/1463270‑1463560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑