Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,707,288:1 +A 9.1% intergenic (+106/+36) PP_1501 → / ← PP_1502 hypothetical protein/OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,707,2881.A 9.1% 291.7 / 22.3 77intergenic (+106/+36)PP_1501/PP_1502hypothetical protein/OmpA family protein
Reads supporting (aligned to +/- strand):  ref base . (35/35);  new base A (3/4);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

GGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGT  >  NC_002947/1707140‑1707430
                                                                                                                                                     |                                                                                                                                                
ggTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCcgcg                                                                                                                                                   >  3:149809/1‑149 (MQ=255)
 gTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑t                                                                                                                                                 <  4:122184/149‑1 (MQ=255)
  tGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACGGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑Tc                                                                                                                                                >  3:361012/1‑149 (MQ=255)
  tGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑Tc                                                                                                                                                >  5:31107/1‑149 (MQ=255)
      cTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATc                                                                                                                                            >  6:294750/1‑149 (MQ=255)
           tGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCggg                                                                                                                                       <  4:36648/149‑1 (MQ=255)
            gggCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCgggg                                                                                                                                      >  4:307166/1‑149 (MQ=255)
             ggCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGA‑CGCGGGGc                                                                                                                                     <  5:242093/149‑1 (MQ=255)
               cGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCt‑‑t                                                                                                                                 <  7:218935/149‑1 (MQ=255)
                  ggAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGCGCGCCCCGCGGGGGGCGCTCGGACTCACAGGGGGCCCCACATTTTCGGCGAAAAAAAAAAAGCCCCCCG‑GGGAGCCCGGGGGT‑‑TGTc                                                                                                                              >  2:192869/1‑149 (MQ=255)
                  ggAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGA‑CGCGGGGCT‑‑TTTc                                                                                                                              >  1:164234/1‑149 (MQ=255)
                  ggAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTc                                                                                                                              <  2:49434/149‑1 (MQ=255)
                   gAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTcc                                                                                                                             <  1:243573/149‑1 (MQ=255)
                   gAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTcc                                                                                                                             >  1:64950/1‑149 (MQ=255)
                     aaCACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGA‑CGCGGGGCT‑‑TTTCttt                                                                                                                           <  5:390519/149‑1 (MQ=38)
                      acacCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGA‑CGCGGGGCT‑‑TTTCtttg                                                                                                                          <  2:164234/149‑2 (MQ=38)
                        acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCttt                                                                                                                          >  1:55800/1‑147 (MQ=255)
                          cgcgGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAg                                                                                                                       >  1:334519/1‑148 (MQ=255)
                              gCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTag                                                                                                                   >  8:98986/1‑148 (MQ=255)
                              gCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTag                                                                                                                   <  7:98986/148‑1 (MQ=255)
                                  gTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCt‑‑t                                                                                                                                 >  7:284571/1‑130 (MQ=255)
                                  gTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCt‑‑t                                                                                                                                 <  8:284571/130‑1 (MQ=255)
                                  gTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTagccagc                                                                                                              >  8:211491/1‑149 (MQ=255)
                                  gTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTagccagc                                                                                                              >  4:261228/1‑149 (MQ=255)
                                       gTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAg                                                                                                         >  4:157740/1‑149 (MQ=255)
                                        tGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAgc                                                                                                        <  1:159612/149‑1 (MQ=255)
                                        tGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑GCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAgc                                                                                                        >  1:136323/1‑149 (MQ=255)
                                          aGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAgcgc                                                                                                      <  6:31107/149‑1 (MQ=255)
                                             tttCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGa                                                                                                   <  4:301158/149‑1 (MQ=255)
                                                cGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCGTTTCAGCTAGCCAGCCTTAGCGCTGAAc                                                                                                 <  5:294750/148‑1 (MQ=255)
                                                  tcgcATCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGTCGGCACAACATTATCGGCGAACAAAGAAAAGGCCCTCG‑TCTATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTg                                                                                              <  8:54112/148‑1 (MQ=39)
                                                  gcgcAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTg                                                                                              <  6:317139/149‑1 (MQ=255)
                                                   cgcAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTgg                                                                                             <  2:334519/149‑1 (MQ=255)
                                                         aTTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCAcc                                                                                       <  7:155558/149‑1 (MQ=255)
                                                             ttGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGc                                                                                   >  3:50247/1‑149 (MQ=255)
                                                                gcgcCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtg                                                                                <  3:261228/149‑1 (MQ=255)
                                                                  gcCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtggt                                                                              <  7:360840/149‑1 (MQ=255)
                                                                     cgcgAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGc                                                                            >  2:9212/1‑148 (MQ=255)
                                                                      gcgAGATCGAGCGCCGCGCGGGCGGCGCTCGAGCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCGTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCa                                                                          >  3:108529/1‑149 (MQ=255)
                                                                            tCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCt                                                                    >  4:243168/1‑149 (MQ=255)
                                                                              gAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGCTCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTgg                                                                  >  7:27044/1‑149 (MQ=255)
                                                                              gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTgg                                                                  >  7:50460/1‑149 (MQ=255)
                                                                               cgcgcCCCCCGCGCGGCGCTCGATCTCGGGGGGGGCAAAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCTTCTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTg                                                                   <  1:116832/148‑1 (MQ=255)
                                                                               aGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGt                                                                 >  8:247934/1‑149 (MQ=255)
                                                                               aGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGt                                                                 >  8:17238/1‑149 (MQ=255)
                                                                                gcgcCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGtt                                                                >  5:22544/1‑149 (MQ=255)
                                                                                gcgcCGCCCGCGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGtt                                                                <  8:50460/149‑1 (MQ=255)
                                                                                   ccgcGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTc                                                             <  3:307166/149‑1 (MQ=255)
                                                                                   ccgcGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTc                                                             <  5:48953/149‑1 (MQ=255)
                                                                                   ccgcGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTc                                                             >  3:123768/1‑149 (MQ=255)
                                                                                        cgggcggCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTgg                                                        <  4:50247/149‑1 (MQ=255)
                                                                                        cgggcggCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTgg                                                        <  3:157740/149‑1 (MQ=255)
                                                                                            cggcgCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGAt                                                    <  1:192869/149‑1 (MQ=255)
                                                                                             ggcgCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATg                                                   <  6:22544/149‑1 (MQ=255)
                                                                                             ggcgCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATg                                                   <  4:123768/149‑1 (MQ=255)
                                                                                                gcTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCttt                                                                                                                          >  7:292958/1‑75 (MQ=255)
                                                                                                gcTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCttt                                                                                                                          <  8:292958/75‑1 (MQ=255)
                                                                                                 cTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCa                                               >  4:134955/1‑149 (MQ=255)
                                                                                                    gATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCAc                                              <  4:149809/147‑1 (MQ=255)
                                                                                                    gATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCt                                            >  3:148662/1‑149 (MQ=255)
                                                                                                         cacaGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCAcc                                       >  5:4841/1‑149 (MQ=255)
                                                                                                          acgggcggcACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTc                                           <  4:361012/141‑1 (MQ=255)
                                                                                                          acaGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCAccc                                      >  6:394023/1‑149 (MQ=255)
                                                                                                               cggcACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGGGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACgg                                 <  2:136323/149‑1 (MQ=255)
                                                                                                                   acaacaTTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCt                             <  6:4841/149‑1 (MQ=255)
                                                                                                                              ggCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTccc                  >  8:48179/1‑149 (MQ=255)
                                                                                                                                 gAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAg               >  1:170395/1‑149 (MQ=255)
                                                                                                                                   aCAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTGCCGAGtt             >  1:372939/1‑149 (MQ=255)
                                                                                                                                   aCAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGtt             <  7:247934/149‑1 (MQ=255)
                                                                                                                                   aCAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGtt             <  8:27044/149‑1 (MQ=255)
                                                                                                                                    cAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTg            <  3:243168/149‑1 (MQ=255)
                                                                                                                                       aGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTc         <  5:394023/149‑1 (MQ=255)
                                                                                                                                       aGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTc         >  3:76042/1‑149 (MQ=255)
                                                                                                                                         acaaGCCCCGCGATCGA‑CGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTc                                                <  5:262090/106‑1 (MQ=39)
                                                                                                                                         acaaGCCCCGCGATCGA‑CGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTc                                                >  6:262090/3‑108 (MQ=39)
                                                                                                                                         aaaaGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCTCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCAGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTATGCGCACGTCCCGACTTGcac         >  3:280417/1‑145 (MQ=255)
                                                                                                                                            aGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGtt    <  2:55800/149‑1 (MQ=255)
                                                                                                                                             gCCCCGCGATCGA‑CGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTg   >  1:261404/1‑149 (MQ=255)
                                                                                                                                              ccccGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGt  >  6:342967/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCG‑TCGATCGCGGGGCT‑‑TGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGT  >  NC_002947/1707140‑1707430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑