| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,932,638:1 | +C | 100% | intergenic (‑105/+45) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 97.9% | 155.0 / ‑3.1 | 48 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (24/23); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT > NC_002947/1932490‑1932781 | aTGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTg < 5:84905/149‑1 (MQ=255) ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAggg > 7:328146/1‑149 (MQ=255) gAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCCCTCCAAAAAAAGATATTCGTCCCCAGTAGGAGACGGCTTGCCC‑GCGAAAGGCTg > 5:239449/1‑149 (MQ=255) gATCTGGAAATTGCACTCCGCCATTGCAGGTCACACTAAAATACCAGAAACCGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCACGCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGCGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGc < 8:328146/149‑1 (MQ=25) gACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTGCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGCCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCa < 1:38708/144‑1 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCaaa > 6:119025/1‑149 (MQ=255) ttCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAAt > 1:37718/1‑149 (MQ=255) gACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGt > 1:151515/1‑149 (MQ=255) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGGCAAGCCCGCTCAAAAAAAGGATATGCGTACCCTGTAGGGGCGGGGTTGCCC‑GCCAAGGGCTGCAAGGCAGCCCCAAAtttt > 2:125413/1‑147 (MQ=255) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 3:310506/149‑1 (MQ=255) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCGTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 5:148804/149‑1 (MQ=255) cAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGAt > 3:57820/1‑149 (MQ=255) ggTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTAAAAAGTATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa < 2:37718/149‑1 (MQ=255) ctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGc < 4:57820/148‑1 (MQ=255) gAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg > 8:302896/1‑149 (MQ=255) tAAACGCTGGGGCCGCTTGGCGGCCCTGCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg < 3:100155/149‑1 (MQ=255) aaCGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt < 5:146260/149‑1 (MQ=255) gCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑cccgc < 7:143082/76‑4 (MQ=255) tGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGa < 5:119025/148‑1 (MQ=255) cGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagca < 2:151515/149‑1 (MQ=255) cGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGag > 7:168583/1‑147 (MQ=255) ggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtt > 5:97669/1‑149 (MQ=255) gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt > 3:230365/1‑149 (MQ=255) aaGCCCGCTCCTACAAAGCAATTGCGTCCCGTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 8:103839/149‑1 (MQ=39) gCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg > 4:238024/1‑112 (MQ=255) gCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg < 3:238024/112‑1 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 5:174446/1‑149 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc < 6:97669/149‑1 (MQ=255) gCTCCTAAAAAGCATTGGCGGCCCCTGTAGGCGGGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc < 6:239449/149‑1 (MQ=255) tCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGa > 6:137876/1‑149 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGTAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcag < 3:306219/125‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcag > 4:306219/1‑125 (MQ=255) tACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCAGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCAAGAATCGAGCAGCTGACCTGGTTTTCCAGCACGatat > 6:185617/1‑149 (MQ=39) cAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg < 5:137876/148‑1 (MQ=255) gATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcag > 1:278927/1‑117 (MQ=255) gATATGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcag < 2:278927/117‑1 (MQ=255) atatGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGGGCGATATGCCcatca > 8:130421/1‑149 (MQ=39) atGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcaca > 2:8638/1‑144 (MQ=255) atGCGTCCCCTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcaca < 1:8638/144‑1 (MQ=255) cccTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc > 1:38067/1‑148 (MQ=255) ccTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatcat < 1:259792/140‑1 (MQ=255) ccTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatcat > 2:259792/1‑140 (MQ=255) cTGTAGGAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 3:120050/1‑149 (MQ=255) ggAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca < 2:38067/149‑1 (MQ=255) ggAGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 4:230365/148‑1 (MQ=255) aGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcaca > 4:179710/1‑149 (MQ=255) aGCGGGCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcaca < 3:273397/149‑1 (MQ=255) ggCTTGCCC‑GCGAAGGGCTGCAAGGCCGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGCTTATCGCGCTCGAGATGCACATCATCCTAGCCCATGTTCACATAtt > 2:63561/1‑149 (MQ=255) gCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAttt > 7:127532/1‑149 (MQ=255) gCTTGCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAttt < 8:168583/149‑1 (MQ=255) | ATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT > NC_002947/1932490‑1932781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |