Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,408,053:1	+T	5.7%	coding (491/615 nt)	PP_3021 ←	LysE family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,408,053	1	.	T	5.7%	290.6 / 10.0	70	coding (491/615 nt)	PP_3021	LysE family transporter
Reads supporting (aligned to +/- strand):  ref base . (31/35);  new base T (2/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

GACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGAC  >  NC_002947/3407907‑3408193                                                                                                                                                         |                                                                                                                                              gACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑Cg                                                                                                                                             >  7:163129/1‑149 (MQ=255)  aCATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCAAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑Cgg                                                                                                                                            >  4:172987/1‑149 (MQ=255)   cATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGc                                                                                                                                           <  3:369380/149‑1 (MQ=255)      gcgcACCTTGCAACTAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGAc                                                                                                                                        >  7:328787/1‑149 (MQ=255)       cgcACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGAc                                                                                                                                        <  8:280357/148‑1 (MQ=255)            cTTGCAACAAGTCAGCCGCGGGCCAGATGCAGCAACAACGCCCCCGCCTCCATCACCCGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGGTGCCTA‑‑‑TG‑‑GCCA‑CGGCGACCAGCaa                                                                                                                                  <  4:52647/149‑1 (MQ=255)            cTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGcgg                                                                                                                                  <  4:143978/149‑3 (MQ=255)             ttGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAg                                                                                                                                 >  8:270320/1‑149 (MQ=255)               gCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGt                                                                                                                                <  3:265716/148‑1 (MQ=255)                 aacaaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCa                                                                                                                                      <  3:2599/140‑1 (MQ=255)                 aacaaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCa                                                                                                                                      >  4:2599/1‑140 (MQ=255)                   caaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCag                                                                                                                           >  1:381827/1‑149 (MQ=255)                    aaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCaga                                                                                                                          <  8:212652/149‑1 (MQ=255)                          gCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑a                                                                                                                   <  7:119047/149‑1 (MQ=255)                                   cAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGa                                                                                                          >  6:306739/1‑149 (MQ=255)                                     gATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAAc                                                                                                        <  7:221305/149‑1 (MQ=255)                                         cagcaACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATc                                                                                                     <  3:366414/148‑1 (MQ=255)                                           gcaACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCa                                                                                                   >  1:158110/1‑148 (MQ=255)                                               caacGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCTCTCCAGCCACGCCAGGCTGCGTGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAgcg                                                                                              <  5:299805/149‑1 (MQ=255)                                               caacGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAgcg                                                                                              <  1:133069/149‑1 (MQ=255)                                                aacgccaacgccAACATCACCACCATGACCCCCGAGGCCCGCACCAGCCACGCCAAGCAGCGGGCGAAGCGCCGCAGAACCCCCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCACCAACTACCAGAGCAAG‑ACGATGCTGAACAACCATAgcgc                                                                                             >  5:273811/1‑149 (MQ=255)                                                  cGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGcgcg                                                                                            >  6:288849/1‑148 (MQ=255)                                                     ccccGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCTAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAgg                                                                                        <  5:299921/149‑1 (MQ=255)                                                     ccccGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAgg                                                                                        >  2:211511/1‑149 (MQ=255)                                                        cGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCg                                                                                     >  3:133610/1‑149 (MQ=255)                                                         gCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGt                                                                                    >  1:29018/1‑149 (MQ=255)                                                          ccAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGtt                                                                                   <  8:163129/149‑1 (MQ=255)                                                               aTCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTc                                                                                >  2:263305/1‑147 (MQ=255)                                                                   ccAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGGTTTCCAGCCg                                                                          >  1:49137/1‑149 (MQ=255)                                                                      gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGTGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       <  6:273811/149‑1 (MQ=255)                                                                      gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       <  5:162168/149‑1 (MQ=255)                                                                      gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       <  1:211511/149‑1 (MQ=255)                                                                      gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       <  5:306739/149‑1 (MQ=255)                                                                       cATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGc                                                                      <  1:62115/149‑1 (MQ=255)                                                                         tGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCAAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCACG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTg                                                                    <  1:248803/149‑1 (MQ=255)                                                                             ccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       >  7:353242/1‑142 (MQ=255)                                                                             ccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                       <  8:353242/142‑1 (MQ=255)                                                                             ccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGc                                                                >  5:42740/1‑149 (MQ=255)                                                                               ccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTg                                                              <  2:49137/149‑1 (MQ=255)                                                                                cGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGCTATCGATTCTGTTGCAA‑GGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGAc                                                                  <  1:126909/149‑1 (MQ=255)                                                                                  aGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CCGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGc                                                            >  6:23046/1‑148 (MQ=255)                                                                                     cccGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGAc                                                         >  2:324329/1‑148 (MQ=255)                                                                                         cTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGc                                                    <  2:29018/149‑1 (MQ=255)                                                                                               ccACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCTGCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGcgg                                                                                                                                  <  1:194208/66‑3 (MQ=17)                                                                                               ccACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCTGCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGcgg                                                                                                                                  >  2:194208/1‑64 (MQ=17)                                                                                                cACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAg                                             >  7:173617/1‑149 (MQ=255)                                                                                                 aCGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAgg                                            >  7:75051/1‑149 (MQ=255)                                                                                                   gCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTg                                                              >  1:149884/1‑129 (MQ=255)                                                                                                   gCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTg                                                              <  2:149884/129‑1 (MQ=255)                                                                                                        gCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCAt                                     <  6:93209/149‑1 (MQ=255)                                                                                                              ggCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCATG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACa                               <  3:172987/149‑1 (MQ=255)                                                                                                                cGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAgc                             <  7:270320/149‑1 (MQ=255)                                                                                                                    gcgcCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGTACGATGCTGAACATCCATAGCGGGTAGGCGGTTTTCCAGCCGGGGATGACGGTGGGGACCATGCTGGCCAGGCTGGCATAGAACAGggcg                          >  1:112115/1‑149 (MQ=255)                                                                                                                    gcgcCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCAAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGt                         >  5:274891/1‑149 (MQ=255)                                                                                                                      gccgcAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTc                       >  5:201797/1‑149 (MQ=255)                                                                                                                          cAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgg                   <  5:23046/149‑1 (MQ=255)                                                                                                                           aGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTggg                  <  4:193433/149‑1 (MQ=255)                                                                                                                            gTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgggg                 <  1:263305/149‑1 (MQ=255)                                                                                                                            gTACCCACTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgggg                 >  4:123538/1‑149 (MQ=255)                                                                                                                               cccGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGtt               <  5:288849/148‑1 (MQ=255)                                                                                                                               cccGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTg              <  2:381827/149‑1 (MQ=255)                                                                                                                                ttgCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGtt               >  3:370751/3‑147 (MQ=255)                                                                                                                                      gTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGc       >  4:69637/1‑149 (MQ=255)                                                                                                                                       ttGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGcc      <  6:201797/149‑1 (MQ=255)                                                                                                                                         gCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCa     <  2:158110/148‑1 (MQ=255)                                                                                                                                            gA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCCTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGAc  >  7:343847/1‑148 (MQ=255)                                                                                                                                             a‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAgc                             <  6:241415/120‑1 (MQ=255)                                                                                                                                             a‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAgc                             >  5:241415/1‑120 (MQ=255)                                                                                                                                                 tG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGAAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGCCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGAc  >  1:220071/1‑146 (MQ=255)                                                                                                                                                      ccA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTgg                                                             >  4:298610/1‑84 (MQ=255)                                                                                                                                                         |                                                                                                                                              GACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCCAGAC  >  NC_002947/3407907‑3408193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑