| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,845,703:1 | +G | 100% | intergenic (‑70/+28) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 94.4% | 112.6 / 0.2 | 36 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (24/10); total (25/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACC > NC_002947/3845557‑3845838 | gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑gg < 3:204584/149‑1 (MQ=255)gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑gg > 6:112202/1‑149 (MQ=255) tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑gggc < 8:257798/149‑3 (MQ=255) gctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGCCGGGCAGAAAAGACGTGTTTC‑GC‑gggct > 4:303503/1‑146 (MQ=255) cGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATc > 5:388238/1‑148 (MQ=255) cATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATc > 8:93548/1‑149 (MQ=255) cATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATc < 1:234608/149‑1 (MQ=255) tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAg > 3:272641/1‑149 (MQ=255) tGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCAc > 7:133850/1‑148 (MQ=255) cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTGATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGATTC‑GCGGG‑GTGATCCATCGTCAGCCACGAt < 1:34453/149‑1 (MQ=255) cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGAt > 6:122710/1‑149 (MQ=255) cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGAt > 5:218036/1‑149 (MQ=255) tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGAt > 2:305732/1‑148 (MQ=255) tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGAt > 8:18484/1‑148 (MQ=255) ctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGAt > 3:186362/1‑147 (MQ=255) cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCa < 5:112202/146‑1 (MQ=255) tCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTTATGCATACTAAGCCACGATGGGTAAGCCCGCcccc > 1:172521/1‑149 (MQ=255) cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCg > 4:114969/1‑149 (MQ=255) gACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCgg > 3:107021/1‑149 (MQ=255) ttCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTg > 3:83238/1‑148 (MQ=255) tcattgttattcttGTCATAGAGGTCTCTCTGTCTAAAGCGTTGCGCCAGACTAAGAGCTGGATGACGTGCAGAAAAGAAGTGTTTC‑GC‑GGTCTGATCCATGGTCAGACACGAAGTATCATACAGCGCCCTGCCATG‑CCGTTGCAAgc > 8:269241/3‑148 (MQ=255) attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACgcag > 3:44577/1‑148 (MQ=255) tgtCAGCGGGGTCTCTCTGTCTGAAGCGTTGCGCCATACTAAGCGCTGTTTGAGGGGCATAAACGAGGTGTTTCGGG‑GG‑CTTATCCATCGTCAGCCACTATGTATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAggtggt < 4:107021/148‑1 (MQ=255) tctctcTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CATATCCATGGTCAGCCACGATGGGTGCGCCCGCGCCCGGCCCGG‑CCGGGGGACGCCGCACCAGGTGGGCAAGCGGGcccc > 7:132886/1‑149 (MQ=255) tctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAggtg > 5:42094/1‑131 (MQ=255) tctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagcc < 7:93548/146‑1 (MQ=255) tgAAGCGTTGCGCCAGACTAAGTGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑ATGATACATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAAAAGGGTGTCAAGAAGGCGCCGCGCCGACAg > 7:59855/1‑149 (MQ=255) aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc > 1:162010/1‑148 (MQ=255) aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc < 7:18484/148‑1 (MQ=255) gTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGtg > 2:92520/1‑64 (MQ=255) gTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGtg < 1:92520/65‑2 (MQ=39) gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt > 7:20918/1‑149 (MQ=255) aGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGACCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGc < 1:305732/149‑1 (MQ=255) gACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCg < 4:83238/149‑1 (MQ=255) tAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCAACGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTc > 1:91533/1‑149 (MQ=255) gagCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcgcg < 3:114969/149‑1 (MQ=255) ggATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCgcgc > 6:278209/1‑145 (MQ=255) ggATGACGGGCAGAAAAGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCgcgc < 5:278209/145‑1 (MQ=255) aGACGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcgc > 5:327109/1‑127 (MQ=255) aCGTGTTTC‑GCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCAcc > 3:304786/1‑147 (MQ=255) | GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGG‑CCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACC > NC_002947/3845557‑3845838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |