Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,962,582:1	+T	7.9%	coding (780/1152 nt)	PP_3492 →	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,962,582	1	.	T	7.9%	244.9 / 12.9	63	coding (780/1152 nt)	PP_3492	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (29/29);  new base T (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGCGCAGTGC  >  NC_002947/3962438‑3962728                                                                                                                                                   |                                                                                                                                                   ggTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGaatgcgg                                                                                                                                                >  5:54786/1‑144 (MQ=255)  gTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCAAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCTCCCGCGCCGCATTCGAA‑gcggc                                                                                                                                               <  4:369918/149‑1 (MQ=255)   tCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑gcgg                                                                                                                                                <  2:185496/147‑1 (MQ=255)      ccTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTg                                                                                                                                           <  6:62001/149‑1 (MQ=255)      ccTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGC‑TTCGAATGCGgcgcgg                                                                                                                                           <  1:50025/149‑3 (MQ=255)        ttCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGt                                                                                                                                         >  6:282345/1‑149 (MQ=255)           gACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGAATGCGGCGCgggcgat                                                                                                                                      <  6:54786/149‑5 (MQ=255)            aCAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTACCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTAc                                                                                                                                     <  1:58786/149‑1 (MQ=255)                  gCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGt                                                                                                                               >  1:63853/1‑149 (MQ=255)                   ccGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGt                                                                                                                               >  2:6715/1‑148 (MQ=255)                   ccGCATGCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGt                                                                                                                               <  3:76248/148‑1 (MQ=255)                    cGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGa                                                                                                                             >  5:390590/1‑149 (MQ=255)                                gccAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGtt                                                                                                                 >  6:1850/1‑149 (MQ=255)                                 ccAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGttt                                                                                                                <  2:250963/149‑1 (MQ=255)                                    atatCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGc                                                                                                             <  6:257407/149‑1 (MQ=255)                                     tatCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCa                                                                                                            <  2:362081/149‑1 (MQ=255)                                      atCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAAGGGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGc                                                                                                             >  1:295663/1‑148 (MQ=255)                                       tCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGGAAg                                                                                                          >  8:240659/1‑149 (MQ=255)                                        ccTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGAATGCGGCGCggg                                                                                                                                          >  2:138152/1‑116 (MQ=255)                                        ccTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCAGCGCCGC‑TTCGAATGCGGCGCggg                                                                                                                                          <  1:138152/116‑1 (MQ=38)                                        ccTCGGTGAGCGTGGCAAGGGCCTGGCCATCGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGc                                                                                                         >  8:211858/1‑149 (MQ=255)                                            ggggcgCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCg                                                                                                       <  2:295663/142‑1 (MQ=255)                                            ggTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGAt                                                                                                     <  2:63853/149‑1 (MQ=255)                                                gcaatGGCCAGGCCCTTGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                                 >  3:265659/5‑149 (MQ=255)                                                aGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                                 <  4:186606/149‑1 (MQ=255)                                                aGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAAc                                                                                                 <  1:6715/149‑1 (MQ=255)                                                   gTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGaa                                                                                              <  8:5145/149‑1 (MQ=255)                                                      gCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGCacac                                                                                           >  2:126901/1‑149 (MQ=255)                                                        aaGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCa                                                                                         >  2:43609/1‑149 (MQ=255)                                                              cTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGa                                                                                                      >  5:96040/1‑130 (MQ=255)                                                              cTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGa                                                                                                      <  6:96040/130‑1 (MQ=255)                                                               tGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCAtt                                                                                  >  8:209775/1‑149 (MQ=255)                                                                 gCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGc                                                                                >  6:335110/1‑149 (MQ=255)                                                                        cccTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTg                                                                                                               >  2:214114/1‑111 (MQ=255)                                                                        cccTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTg                                                                                                               <  1:214114/111‑1 (MQ=255)                                                                           tGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCAtt                                                                                  >  8:177340/1‑137 (MQ=255)                                                                           tGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCAtt                                                                                  <  7:177340/137‑1 (MQ=255)                                                                             tCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTgg                                                                     <  6:39197/148‑1 (MQ=255)                                                                              ccAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGcc                                                                   >  2:388596/1‑149 (MQ=255)                                                                                aaCCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGa                                                                 >  8:146365/1‑149 (MQ=255)                                                                                  ccTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACa                                                               <  1:200639/149‑1 (MQ=255)                                                                                   cTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAt                                                              >  7:67822/1‑149 (MQ=255)                                                                                      gAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgc                                                            <  1:43609/148‑1 (MQ=255)                                                                                       aa‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgcag                                                          >  6:33652/1‑149 (MQ=255)                                                                                              gcCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACAtgcaggtgcag                                                    <  5:1850/149‑1 (MQ=255)                                                                                                cGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTAGAA‑GCGGCGCTGGTCTACTCGCGAGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCAATGCCAACCTGCTGGCCGACATtcaggtgcaggt                                                  >  4:80740/1‑149 (MQ=255)                                                                                                             ccgccCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGt                                     >  5:387396/1‑149 (MQ=255)                                                                                                               gccCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGttt                                   <  7:209775/149‑1 (MQ=255)                                                                                                                cccAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTg                                  >  5:204597/1‑149 (MQ=255)                                                                                                                  cAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCt                                >  6:379310/1‑149 (MQ=255)                                                                                                                      ccTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATc                            <  5:33652/149‑1 (MQ=255)                                                                                                                        ttGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAAc                                                                            <  8:299375/99‑1 (MQ=255)                                                                                                                        ttGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAAc                                                                            >  7:299375/1‑99 (MQ=255)                                                                                                                        ttGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTAGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCt                          >  8:159900/1‑149 (MQ=255)                                                                                                                          gggCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCt                          <  8:67822/147‑1 (MQ=255)                                                                                                                           ggCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGc                        <  7:211858/148‑1 (MQ=255)                                                                                                                           ggCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGc                        <  6:387396/148‑1 (MQ=255)                                                                                                                            gCAGCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCAt                      <  5:394430/149‑1 (MQ=255)                                                                                                                                  cccGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATgccgcc                >  8:277629/1‑149 (MQ=255)                                                                                                                                  cccGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATgccgcc                >  2:201526/1‑149 (MQ=255)                                                                                                                                   cagcgcCGC‑TTCGAATGCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGccgccc               >  7:123736/3‑149 (MQ=255)                                                                                                                                    cgcgCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGccgcccg              <  7:146365/149‑1 (MQ=255)                                                                                                                                         cgcATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTgc         >  1:204761/1‑149 (MQ=255)                                                                                                                                          gcATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGc                                 <  6:6403/124‑1 (MQ=255)                                                                                                                                              agtaa‑GCGGGGTTGGTCT‑CTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGCGCAGt    <  6:30768/145‑1 (MQ=255)                                                                                                                                               cGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGCGCAGt    <  2:243664/148‑1 (MQ=255)                                                                                                                                                gAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGCGCAGTGc  <  8:232874/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GGTCACCTTCGACAACTGCCGCATTCCTGCCGCCAATATCCTCGGTGAGCGTGGCAAGGGCCTGGCCATTGCCCTGTCCAACCTCGAA‑GGCGGCCGTATCGGCATTGCCGCCCAGGCCTTGGGCATCGCCCGCGCCGCATTCGAA‑GCGGCGCTGGTCTACTCGCGTGACCGCATACAGTTTGGCAAGCCGATCAACGAACACCAGAGCATTGCCAACCTGCTGGCCGACATGCAGGTGCAGGTGAACGCCGCACGTTTGCTGATCCTGCATGCCGCCCGCCTGCGCAGTGC  >  NC_002947/3962438‑3962728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑