| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 278,742:1 | +A | 100% | intergenic (‑237/+77) | PP_0224 ← / ← sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 278,742 | 1 | . | A | 96.3% | 174.3 / ‑0.2 | 54 | intergenic (‑237/+77) | PP_0224/sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (22/30); total (24/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGG > NC_002947/278597‑278881 | gTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑aaa > 4:219408/1‑148 (MQ=255) tCGCTACAGCTGATCGCCACAGACGTGTTCTGGTCCGGTTGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑aaac < 7:160934/149‑3 (MQ=255) cAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCAGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTc < 2:180445/149‑1 (MQ=255) aCAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGc < 2:213095/149‑1 (MQ=255) aGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCt < 3:236240/149‑1 (MQ=255) cTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTac > 2:50285/1‑130 (MQ=255) cTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTAATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTac < 1:50285/130‑1 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCtgtg < 7:23826/148‑1 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCtgt < 1:163029/147‑1 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCtgt < 4:72075/147‑1 (MQ=255) tccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCa < 3:219408/149‑1 (MQ=255) cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTcc > 2:111171/1‑121 (MQ=255) cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAACATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTcc < 1:111171/121‑1 (MQ=255) tggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa < 8:114733/149‑1 (MQ=255) tatatataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑ggg < 5:254033/149‑1 (MQ=255) tatatataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑ggg < 6:386044/149‑1 (MQ=255) tatataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGtt < 8:238579/149‑1 (MQ=255) aCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑t < 6:158707/92‑1 (MQ=255) aCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑t > 5:158707/1‑92 (MQ=255) cTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACAAATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCcgc > 5:148690/1‑148 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAg < 6:116484/116‑1 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAg > 5:116484/1‑116 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCt > 6:122848/1‑120 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCt < 5:122848/120‑1 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa > 6:253549/1‑130 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa < 5:253549/130‑1 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑gg < 2:41689/137‑1 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑gg > 1:41689/1‑137 (MQ=255) tataAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGcc < 6:148690/148‑1 (MQ=255) tataAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCg > 6:352967/1‑149 (MQ=255) ttaAAATTTTTATGAACTAATTTTTTGTATTATTTTTTGAAGGGGTTTTTGTTAGAGTGGTGCCGTGCTCTTCTTGTT‑TAAACCCGCTCGTACAAACATGCATCATGTTCTTTGCACTTTAGGTGC‑GGTTTTACCCGCGAAAGGGCCg < 8:3835/147‑1 (MQ=255) aaaaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCtgt < 1:37869/111‑1 (MQ=255) aaaaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCtgt > 2:37869/1‑111 (MQ=255) aTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAg < 5:352967/149‑1 (MQ=255) ttttATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGt > 8:105274/1‑149 (MQ=255) tttATGAACTAACTTTTTGGAATATTTATAGAAGGGGGTTTGTTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGt < 8:254092/148‑1 (MQ=39) tttATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAAACCCCAACATCACACAAGCCTGAAGATATGTGGAATGTAGGAAC‑GGGTTTTCCCGCGAAAAGCTCGGAagagcgt > 2:293924/1‑146 (MQ=255) ttaaTGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGaa < 1:293924/130‑1 (MQ=255) ctaactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTT‑AAACCGCTGCTAAAAACATGGCTCAAGATATGTGCAATGTTGGAGC‑GGGTTTAACCGGGAAATGGCCGGAACAGGttttatgg > 3:201806/1‑142 (MQ=255) ctaactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATATGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTaatag > 3:178800/1‑143 (MQ=255) taactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGttt > 8:74801/1‑115 (MQ=255) taactTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGttt < 7:74801/115‑1 (MQ=255) actTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCcgc < 6:177919/119‑1 (MQ=255) actTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCcgc > 5:177919/1‑119 (MQ=255) gAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTT‑AAACCCCGCCAACACACAAGGCGCAAGAAGAGTGGCATGTTGGGGCGGGGTTTACCCCGAAAAGGGCGGGAACA‑GTTTAATCGGGGGCCGccttac > 2:138648/1‑144 (MQ=255) tttATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACCCACATGCCTCAAGATGTGTGCACTGTAGGAGC‑GGGTTTTCCCGCGaaa > 2:280439/1‑110 (MQ=38) tttATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACCCATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGaaa < 1:280439/110‑1 (MQ=255) ttATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGATAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAgg > 5:328401/1‑148 (MQ=255) ataGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCGACCCACCTGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGCAc > 2:354283/1‑149 (MQ=39) gAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTg > 4:63362/1‑149 (MQ=255) aaGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgc > 1:350826/1‑149 (MQ=255) aGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTgcg < 7:105274/149‑1 (MQ=255) ttACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGGCGCAGTCCCGAGGAACTTGCGCAAGCACTGcc > 2:295708/1‑149 (MQ=255) gTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGc < 3:63362/149‑1 (MQ=255) gggCGCTTCGCGGG‑TAAACCCGCTCCTCCACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTcttc < 3:283280/149‑1 (MQ=255) ttCGCGGG‑TAAACCCGCTCCTACCCACATGCCTCATGATCTGTGCACTGTCGGAGG‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCg < 5:76157/100‑1 (MQ=37) ttCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCgg > 2:27164/1‑149 (MQ=255) ttCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑CGGTTTACCCGCGAAACGGCCGGAACAGCGTTACTCGGAGGCCg > 6:76157/1‑100 (MQ=38) | GTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGG > NC_002947/278597‑278881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |