| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| JC | NC_002947 | 4,980,585 | +GGC | 100% | intergenic (‑24/+44) | PP_4387 ← / ← flgE | hypothetical protein/flagellar hook protein FlgE |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_002947 | = 4980585 | 2 (0.030) | 52 (0.860) +GGC |
45/260 | NT | 96.4% | intergenic (‑24/+44) | PP_4387/flgE | hypothetical protein/flagellar hook protein FlgE |
| ? | NC_002947 | 4980586 = | 2 (0.030) | intergenic (‑25/+43) | PP_4387/flgE | hypothetical protein/flagellar hook protein FlgE | |||||
ACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑‑‑GG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCG > NC_002947/4980447‑4980734 | gaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCt > 6:200195‑M2/146‑149 (MQ=255) caggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagcaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGc > 2:52895‑M2/142‑149 (MQ=255) aggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCg > 5:135127‑M2/141‑149 (MQ=255) ccggtgcccaggctttcccaatcatacaaccgggcggtggacgcgggcgcaggcagaaccagcaccagcaaggggaacaaggccagcaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAAtt < 8:175879‑M2/18‑1 (MQ=255) cggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAAtt > 5:316612‑M2/131‑148 (MQ=255) ggggaccaggctttgcctttgatagaagggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTAc < 6:112873‑M2/20‑1 (MQ=255) gtgaccaggctttgccattgatcgaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACa < 5:377528‑M2/21‑1 (MQ=255) ctgtcccattgatcgaacggggcgggcgcagcggggccaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcccgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCaaa < 3:43675‑M2/30‑1 (MQ=255) cattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCaa > 7:343120‑M2/114‑148 (MQ=255) aacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaccgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAatcat > 6:114120‑M2/105‑148 (MQ=255) aacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAatcat > 6:108818‑M2/105‑148 (MQ=255) ctgggcgggggccgcggtggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgctgcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAg < 8:288737‑M2/47‑1 (MQ=255) cgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCAt < 6:316612‑M2/52‑1 (MQ=255) ggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCt > 4:369095‑M2/96‑149 (MQ=255) ggcagcgggggcaggcagaaccagcacctgcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCg > 1:217965‑M2/94‑102 (MQ=255) ggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCg < 2:217965‑M2/9‑1 (MQ=255) agcgggggcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑‑‑GGGGCCGCTTTTCGGGGCCCATTTCACCCGCCAAAGCCATAAAAAGCAGAAGGTCAGCTG‑Aatg > 8:305999/1‑149 (MQ=255) gcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCa > 8:66818‑M2/93‑143 (MQ=255) gcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCa < 7:66818‑M2/51‑1 (MQ=255) agcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACa < 5:679‑M2/39‑1 (MQ=255) agcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACa > 6:679‑M2/91‑129 (MQ=255) agcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑Aat > 6:45198‑M2/91‑148 (MQ=255) gcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCt > 1:184995‑M2/84‑149 (MQ=255) aggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTg > 3:93632‑M2/82‑148 (MQ=255) aggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGc < 2:184995‑M2/68‑1 (MQ=255) accagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCatgatg < 6:33457‑M2/74‑1 (MQ=255) cagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGc > 4:134106‑M2/73‑140 (MQ=255) cagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGc < 3:134106‑M2/68‑1 (MQ=255) gaaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaacttcacattcttggggcCGCTTTGCGGCCCCAATTACAAAAACAAAAGCAAATACAATCAAGAGGTCATCTG‑AATGAGGGTGTGGATGATGgtgat > 8:177714‑M2/71‑147 (MQ=255) accagcaaggggaacaaggccattaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCttt < 1:271886‑M2/81‑1 (MQ=255) accagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGGCATCTG‑AATGATGGTCTGCATGATGGTGCttt < 2:42597‑M2/81‑1 (MQ=255) gcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCa < 5:186372‑M2/69‑1 (MQ=255) gcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCa > 6:186372‑M2/65‑133 (MQ=255) caaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTg < 6:135127‑M2/86‑1 (MQ=255) aaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAAAACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTg > 3:290535‑M2/63‑148 (MQ=255) ggAACCATGCCATTAACGAAACCCGCATCAATCCCGGGAAACATCACATTAATGGGGG‑‑‑CGGTTTTCGGCCCCAATTACAAACACCAAAGCCAATAAAAACAACAGGGCATCTGAAATGATGGTCTGCATGATGGTGCTTTCAGTGGaaa < 1:52895/149‑1 (MQ=255) ggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGa > 7:162294‑M2/61‑148 (MQ=255) gggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTTCAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGAAGGtca > 8:137225‑M2/60‑124 (MQ=255) gggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCt < 7:137225‑M2/66‑1 (MQ=255) ggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTg < 5:45198‑M2/98‑1 (MQ=255) cagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCa > 6:297758‑M2/49‑149 (MQ=255) agtaacgacagccgcttcattcgcgtgcaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTTCGTTCAGTGGAAATGGGCTTggctt > 4:103689‑M2/48‑147 (MQ=255) aacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCACGTCATCTG‑AATGATGGTCAGCATGATGGTGCTTTCAGTGGAAATGGACTTGGCATTgg > 3:35947‑M2/45‑149 (MQ=255) cagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTgg < 8:343120‑M2/110‑1 (MQ=255) ccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCt > 5:6727‑M2/37‑102 (MQ=255) ccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCt < 6:6727‑M2/66‑1 (MQ=255) gcttcattcgcgtgaaccttcacattcttggggcCGCGTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATAATCAGGTCATCAG‑AATGATGGTCTGCATGAAGGTGCTTTCAGTGGGAATGGTCTTGGCATTGGGCTGGTAttt > 4:39006‑M2/35‑149 (MQ=255) gcttcattcgcctgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAACCACCAAAGCCAATACAATCATCAGCTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGGCTTGGCATTGGCCTGGTAGtt < 4:69462‑M2/115‑1 (MQ=255) cattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGct < 3:39006‑M2/118‑1 (MQ=255) gcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTgcg < 3:369095‑M2/123‑1 (MQ=255) ccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATc < 5:114120‑M2/131‑1 (MQ=255) cattcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATc < 5:108818‑M2/131‑1 (MQ=255) cttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCa < 3:133143‑M2/132‑1 (MQ=255) cacattctttgggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATTGTCTTGGCATTTGCCTGGTAGTTGCTCTGCGCCTTGATCAg < 7:177714‑M2/133‑1 (MQ=255) acattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGt < 8:162294‑M2/135‑1 (MQ=255) ttggggcCGCAAAGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAg > 3:48664‑M2/8‑149 (MQ=255) ggcCGCTTTGCTTCCCCAATTACAAACACCAAAGCCAATACATTCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTg < 8:88054‑M2/116‑1 (MQ=255) ggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGCTCTTGGCATTTGCCTGGTAGTTg > 7:88054‑M2/4‑119 (MQ=255) gcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGc > 6:366614‑M2/3‑149 (MQ=255) cgcTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCg < 1:149537/149‑1 (MQ=255) cgcTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCg < 3:186472/149‑1 (MQ=255) | ACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑‑‑GG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG‑AATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCG > NC_002947/4980447‑4980734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |