Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,477,817	G→C	6.6%	H59D (CAT→GAT)	PP_3968 ←	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,477,817	0	G	C	6.6%	262.4 / 4.9	86	H59D (CAT→GAT)	PP_3968	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (40/40);  new base C (2/4);  total (42/44)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

ATAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTCCCATATC  >  NC_002947/4477670‑4477964                                                                                                                                                    |                                                                                                                                                    aTAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATgc                                                                                                                                                    <  7:110085/149‑1 (MQ=255)    gAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCa                                                                                                                                                 <  4:328401/149‑1 (MQ=255)      agtaccGAGCGCTGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGt                                                                                                                                               >  3:343753/6‑149 (MQ=255)        acTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCt                                                                                                                                             <  4:71948/149‑1 (MQ=255)           cGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGATGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAg                                                                                                                                          >  8:358154/1‑149 (MQ=255)           cGAGCGCAGCGCTCGGTACTCACCGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATAAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCGCATCAGCAGTCTGAg                                                                                                                                          <  3:39868/149‑1 (MQ=255)            gAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCGCATCAGCAGTCTGa                                                                                                                                           <  2:242306/147‑1 (MQ=255)            gAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCGCATCAGCAGTCTGAg                                                                                                                                          <  8:160769/148‑1 (MQ=255)                gctgcgcTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTa                                                                                                                                     <  6:336075/146‑1 (MQ=255)                       tCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCTCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCatat                                                                                                                              <  8:275984/149‑1 (MQ=255)                         ggTACTCATAGACCAATTGATCCCTCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCatat                                                                                                                              <  8:60286/147‑1 (MQ=255)                              tCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCgg                                                                                                                        >  7:338034/1‑148 (MQ=255)                              tCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCgg                                                                                                                        >  2:234177/1‑148 (MQ=255)                                agagACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCaa                                                                                                                     >  2:39217/1‑149 (MQ=255)                                    aCCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGt                                                                                                                 <  4:293107/149‑1 (MQ=255)                                      cAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTTCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTgc                                                                                                               <  2:13687/149‑1 (MQ=255)                                      cAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTgc                                                                                                               >  7:153777/1‑149 (MQ=255)                                        aTTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTgcg                                                                                                              >  6:302714/1‑148 (MQ=255)                                              ccATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAAtc                                                                                                       >  3:203638/1‑149 (MQ=255)                                                aTCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAAtctt                                                                                                     >  3:87468/1‑149 (MQ=255)                                                aTCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTCATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGAAGAAgctt                                                                                                     >  6:229104/1‑149 (MQ=255)                                                     aaaGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAAtcttct                                                                                                   >  4:287009/1‑146 (MQ=255)                                                      aaGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCaga                                                                                               <  2:145859/149‑1 (MQ=255)                                                       aGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCagag                                                                                              <  2:77840/149‑1 (MQ=255)                                                         cccGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGt                                                                                             >  6:30227/1‑148 (MQ=255)                                                             cTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTc                                                                                        <  4:87468/149‑1 (MQ=255)                                                                  aaaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCTGAGTGATTCCTGa                                                                                    <  6:124989/148‑1 (MQ=255)                                                                  aaaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGa                                                                                    <  1:234177/148‑1 (MQ=255)                                                                  aaaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAg                                                                                   >  3:92830/1‑149 (MQ=255)                                                                  aaaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAg                                                                                   >  6:154001/1‑149 (MQ=255)                                                                  aaaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAg                                                                                   <  5:154001/149‑1 (MQ=255)                                                                  aaaCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAg                                                                                   <  5:120737/149‑1 (MQ=255)                                                                    aCGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTATCAGAGTGATTCCTGAGGc                                                                                 >  8:79278/1‑149 (MQ=255)                                                                       aGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCgg                                                                              <  4:343753/149‑1 (MQ=255)                                                                            tGGCATGGGGCTGAGCCGCCGTATCGCGTCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGTGTGATTCCTGAGGCCGGCTGaa                                                                         <  3:259353/149‑1 (MQ=255)                                                                              gCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATc                                                                       <  3:287009/149‑1 (MQ=255)                                                                                    gTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGt                                                                 <  1:64243/149‑1 (MQ=255)                                                                                    gTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATATTCACAGAGAGATTCCTGAGGCCGGCTGAAACCTGAGt                                                                 >  8:56824/1‑149 (MQ=255)                                                                                     tCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTg                                                                >  7:100829/1‑149 (MQ=255)                                                                                     tCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTg                                                                <  8:338034/149‑1 (MQ=255)                                                                                      cTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGc                                                                             >  3:33431/1‑135 (MQ=255)                                                                                      cTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGc                                                                             <  4:33431/135‑1 (MQ=255)                                                                                          gccgccGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGt                                                             >  7:142127/1‑147 (MQ=255)                                                                                           ccgccgTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAg                                                          >  1:161535/1‑149 (MQ=255)                                                                                             gccgTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTc                                                        >  6:79175/1‑149 (MQ=255)                                                                                                 tATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTc                                                    <  8:153777/149‑1 (MQ=255)                                                                                                     gctgctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACTGTGCGCAGAATCTTCTCAGAGTGATTc                                                                                        <  5:178221/109‑1 (MQ=255)                                                                                                     gctgctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACAGTGCGCAGAATCTTCTCAGAGTGATTc                                                                                        >  6:178221/1‑109 (MQ=255)                                                                                                       tgctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGAcc                                              >  5:145806/1‑149 (MQ=255)                                                                                                         ctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGt                                                                                                                 >  5:27700/1‑80 (MQ=255)                                                                                                         ctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGt                                                                                                                 <  6:27700/80‑1 (MQ=255)                                                                                                          tCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGaa                                           >  1:56721/1‑149 (MQ=255)                                                                                                           cATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAAc                                          >  7:73252/1‑149 (MQ=255)                                                                                                            aTTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACg                                         >  7:121617/1‑149 (MQ=255)                                                                                                                gagGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGc                                      <  5:79175/148‑1 (MQ=255)                                                                                                                      ctctGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGc                                      <  6:139703/142‑1 (MQ=255)                                                                                                                      ctctGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGc                                      >  5:139703/1‑142 (MQ=255)                                                                                                                      ctctGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcc                               >  1:337111/1‑149 (MQ=255)                                                                                                                      ctctGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcc                               >  3:318778/1‑149 (MQ=255)                                                                                                                      ctctGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcc                               >  4:231826/1‑149 (MQ=255)                                                                                                                       tctGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcct                              >  5:79373/1‑149 (MQ=255)                                                                                                                         tGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcct                              <  5:302714/147‑1 (MQ=255)                                                                                                                               gcgcCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGc                       <  1:39217/148‑1 (MQ=255)                                                                                                                                gtactgctcAGACTGCGCATCAGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCt                     >  1:171479/7‑149 (MQ=255)                                                                                                                                cgcCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCt                     <  7:56824/149‑1 (MQ=255)                                                                                                                                cagcacatcAGACTGCTCATCAGCAGTCTGAGCAGTACGCATATCAGCGTCAACGTTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGTGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCt                     <  3:382320/142‑1 (MQ=255)                                                                                                                                 gcCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTg                    <  5:30227/149‑1 (MQ=255)                                                                                                                                  ccTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTcc                                                       <  8:189301/113‑1 (MQ=255)                                                                                                                                  ccTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTcc                                                       >  7:189301/1‑113 (MQ=255)                                                                                                                                  ccTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTg                    >  4:370247/1‑148 (MQ=255)                                                                                                                                   cTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGga                  <  3:370247/149‑1 (MQ=255)                                                                                                                                   cTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGga                  <  2:56721/149‑1 (MQ=255)                                                                                                                                        cAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATg             >  5:377060/1‑149 (MQ=255)                                                                                                                                         aGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAAt              <  3:231826/147‑1 (MQ=255)                                                                                                                                         aGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGc            >  7:177546/1‑149 (MQ=255)                                                                                                                                         aGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGc            >  3:291952/1‑149 (MQ=255)                                                                                                                                         aGACTGCGCATCAGCAGTCTGAGAAG‑GCGCATATCAGCCGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAACTCCGTCTTGACCGAACGTGCGAAATACTCCCAAGCCTGGCGAATGCt           >  2:141816/1‑149 (MQ=255)                                                                                                                                           aCTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCtt          <  8:142127/149‑1 (MQ=255)                                                                                                                                           aCTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCtt          <  8:73252/149‑1 (MQ=255)                                                                                                                                            ctgctgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTc         <  6:79373/149‑1 (MQ=255)                                                                                                                                              gctgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTccc       <  4:53610/149‑1 (MQ=255)                                                                                                                                               ctgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTccc       <  7:358154/148‑1 (MQ=255)                                                                                                                                               ctgATGCGCAGTATGAGGAGTACGCATATCAGCGTCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTcc        <  5:229104/147‑1 (MQ=255)                                                                                                                                                tgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGc            <  3:193966/142‑1 (MQ=255)                                                                                                                                                tgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGc            >  4:193966/1‑142 (MQ=255)                                                                                                                                                tgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTCCCa      >  6:311598/1‑148 (MQ=255)                                                                                                                                                 gATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAAAGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTCCCata    >  1:258674/1‑149 (MQ=255)                                                                                                                                                    gcgcAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTCCCATATc  >  4:68108/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ATAGAACACTCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAATGCTTCCCATATC  >  NC_002947/4477670‑4477964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑