Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,503,790 A→G 11.6% intergenic (+39/‑59) xanP → / → tusD xanthine permease/sulfur transfer protein complex subunit TusD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,503,7900AG11.6% 102.6 / 5.7 45intergenic (+39/‑59)xanP/tusDxanthine permease/sulfur transfer protein complex subunit TusD
Reads supporting (aligned to +/- strand):  ref base A (17/21);  new base G (2/3);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

TGCCGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCCGGCGGG  >  NC_002947/4503648‑4503938
                                                                                                                                              |                                                                                                                                                    
tGCCGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGcac                                                                                                                                                     <  8:145921/144‑1 (MQ=255)
tGCCGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGcac                                                                                                                                                     >  7:145921/1‑144 (MQ=255)
   cggcggCGATTCGCAATGTGCTTGAGGCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGTGCAAgcgggc                                                                                                                                             <  8:364931/149‑1 (MQ=255)
     gcggcgATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAgcgggcgc                                                                                                                                           <  5:341099/149‑1 (MQ=255)
     gcggagATTAGCAATGTGCTGGTGTCGGGGGTGGCTACCGGTGGGATTTGTGCGCTGTTGCTGAACTGGTTCTTGCCGTAGGGCAAGGAGCAGGCTTGAACCTTGAGACTTAATGAGAGTAGCCCGCGCCCGCTTGTGCAAgcgggcgc                                                                                                                                           <  8:311830/149‑1 (MQ=255)
       ggcgATTCGCAATGTGCTGGAGTCGGGTGTGGCTTCCGGGGGGTTTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGTGCAAgcgggcgcgg                                                                                                                                         <  2:203465/149‑1 (MQ=255)
                cAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCCGCCCCGCTGGGCCCAGCGGGCGGGGGGgtttttt                                                                                                                                >  6:42746/1‑148 (MQ=255)
                  aTGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGGGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCt                                                                                                                              <  5:36821/149‑1 (MQ=255)
                        tGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGGCCAAGCGGGCGCGGGCTTTTTTGCTTTAACa                                                                                                                        >  4:236606/1‑149 (MQ=255)
                         ggAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCAt                                                                                                                       <  8:365932/149‑1 (MQ=255)
                               gggTGTGGCTACCTGGGGGATTTGTGCGCGGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTAGAACCTTGAGCCTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCAc                                                                                                                  <  4:37224/148‑1 (MQ=255)
                                 gtgtGGCTACCGGGGGGATTTGTGCGCTGGTGTTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATg                                                                                                                      >  1:243003/1‑142 (MQ=255)
                                 gtgtGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATg                                                                                                                      <  2:243003/142‑1 (MQ=255)
                                       cTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCttt                                                                                                         <  4:163264/149‑1 (MQ=255)
                                          ccGGGGGGATTTGTGCGCTGGTGCTGAACTTGTTCTTGCCGGATGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGACTAGCCCGCGCCCGCTTTCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCa                                                                                                      <  5:363527/149‑1 (MQ=255)
                                          ccGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCa                                                                                                      <  4:147693/149‑1 (MQ=255)
                                             gggggATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCttt                                                                                                                            <  3:151426/124‑1 (MQ=255)
                                             gggggATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCttt                                                                                                                            >  4:151426/1‑124 (MQ=255)
                                                     tgtgCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGcc                                                                                            <  1:284601/148‑1 (MQ=255)
                                                              gTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTc                                                                                   <  7:91082/148‑1 (MQ=255)
                                                                   gAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATc                                                                             >  4:120322/1‑149 (MQ=255)
                                                                   gAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATc                                                                             >  7:203312/1‑149 (MQ=255)
                                                                        ggTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCAt                                                                                                     <  5:226673/120‑1 (MQ=255)
                                                                        ggTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGgt                                                                        >  6:310415/1‑149 (MQ=255)
                                                                            cTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAgcgggc                                                                                                                                             >  8:124147/1‑76 (MQ=255)
                                                                            cTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAgcgggc                                                                                                                                             <  7:124147/76‑1 (MQ=255)
                                                                                 cGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCCCGCCCCCTTGGACAAGCCGGCGCCGGGTTTTTTTGGTTATAATGGCCCCAGTCCATTGCATGAGTTAGCCAATAAATTAGGAAAGGCGGTGTTATgccc                                                               >  2:220315/1‑149 (MQ=255)
                                                                                        cAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGGACAAGCGGGCGCGGGGGTTTTTGCTTTATCATGGCACCATTGCTTTGGATGAGTTAGGCATGAAATTCGCGATCGCGGTGTTCTCCCCGGCCCAt                                                        >  2:94292/1‑149 (MQ=255)
                                                                                         aaGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCTGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATg                                                       >  2:213417/1‑149 (MQ=255)
                                                                                             aGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGcc                                                   <  3:120322/149‑1 (MQ=255)
                                                                                                  gCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCtt                                              <  3:236606/149‑1 (MQ=255)
                                                                                                    ttGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTcg                                            >  5:169617/1‑149 (MQ=255)
                                                                                                      gAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTcg                                            <  5:310415/147‑1 (MQ=255)
                                                                                                        aCCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCGTGCACAAGCGGGCGCGGGGTTTTTTGCTTTATCATGGCACCATTGCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGGGTTGTCCCCGGCCCATGCGCCCTCTTCGCGAc                                        >  2:127110/1‑149 (MQ=255)
                                                                                                                  tGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGcc                                                   <  3:128053/128‑1 (MQ=255)
                                                                                                                  tGAATGAGAGTAGCCCGCGCCCCCTTGCACAAACGGGCGCCGGCTTTTTTGCTTTATCATGGCACCATTCCATTGCATGAGTTAGCCATGAAATTAGCTATCGAGGTGTTCTGCCCGGCCCAAGCGcc                                                   >  4:128053/1‑128 (MQ=255)
                                                                                                                       gagaGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCg                         >  5:392769/1‑149 (MQ=255)
                                                                                                                         gagTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGcc                       <  7:312172/149‑1 (MQ=255)
                                                                                                                            tAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAg                    >  4:325032/1‑149 (MQ=255)
                                                                                                                                ccgcgcccgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCgg                <  6:313492/149‑1 (MQ=255)
                                                                                                                                 cgcgcccgcTTGTGCAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACACGCCCTGCGCTTCGCCGAGGCGGt               >  3:274712/1‑149 (MQ=255)
                                                                                                                                  gcgcccgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTg              <  6:392769/149‑1 (MQ=255)
                                                                                                                                     cccgcTTGTGCAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCATTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGGTTCGCCGAGGCGGGGCTg           >  8:191801/1‑149 (MQ=255)
                                                                                                                                      ccgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTgg          >  3:74432/1‑149 (MQ=255)
                                                                                                                                       cgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCTCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGc         >  8:171620/1‑149 (MQ=255)
                                                                                                                                        gcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCATTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCCAGGCGGTGCTGGcc        >  8:177697/1‑149 (MQ=255)
                                                                                                                                            gCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCcgg      <  8:203312/147‑1 (MQ=255)
                                                                                                                                             cacaAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCcggcgg   <  6:169617/149‑1 (MQ=255)
                                                                                                                                             cacaAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCcggcg    <  3:325032/148‑1 (MQ=255)
                                                                                                                                              acaAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCCGGCggg  >  7:220755/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
TGCCGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCCGGCGGG  >  NC_002947/4503648‑4503938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑