| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,530,026 | T→A | 18.0% | intergenic (+32/‑136) | PP_4018 → / → topB | acetyltransferase/DNA topoisomerase III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,530,026 | 0 | T | A | 18.0% | 93.2 / 10.8 | 47 | intergenic (+32/‑136) | PP_4018/topB | acetyltransferase/DNA topoisomerase III |
| Reads supporting (aligned to +/- strand): ref base T (27/11); new base A (3/5); total (30/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGCCTGTTT > NC_002947/4529884‑4530173 | cAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCcgc > 2:112865/1‑149 (MQ=255) aGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTAAACCcgc > 4:304889/1‑148 (MQ=255) tGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTAAACCCGCGAag < 1:351494/149‑1 (MQ=255) cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAaga‑g < 2:95691/149‑1 (MQ=255) cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTAAACCCGCGAaga‑g < 3:304889/149‑1 (MQ=255) cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTAAACCCGCGAaga‑g < 7:359051/149‑1 (MQ=255) cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACCGGCCTCTTCGC‑GGGTTTACCCGCGAaga < 6:358109/148‑1 (MQ=255) cacgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCgg > 8:212971/1‑149 (MQ=255) ccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGGTTACCCGCGAAGA‑GGCCGGCt > 5:388736/1‑147 (MQ=255) tCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGc > 8:335240/1‑148 (MQ=255) tATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCcg < 4:139811/127‑1 (MQ=255) tATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCcg > 3:139811/1‑127 (MQ=255) tttCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGaa < 1:54124/116‑1 (MQ=255) tttCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGaa > 2:54124/1‑116 (MQ=255) gcttcGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCACCGCTGAC‑AAAAAACCt > 3:28904/5‑149 (MQ=255) aGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCAGACTCGCAGGGGTTACCC‑CGAAGACGCCCGGCGCAGGC‑AAATAAATACCCGCGGAA‑AAAAAAccctg > 5:233164/1‑146 (MQ=255) cGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑Ac < 5:158690/149‑1 (MQ=255) gAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑Aca > 6:181856/1‑149 (MQ=255) ggAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGt > 5:67592/1‑144 (MQ=255) ggAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGt < 6:67592/144‑1 (MQ=255) ggAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAAACTGT‑Acatc > 4:124848/1‑149 (MQ=255) aTTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATCTACATCCCCGCTGAC‑AAAAAACCGGT‑Acatccat > 7:285967/1‑149 (MQ=255) tCGAAGCTGGCATCCCGCATGTCGACATGGTGCGTGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑Acatccatc > 2:74951/1‑145 (MQ=255) tCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑Acatccatc > 2:47439/1‑145 (MQ=255) tCGAAGCTGGCATCCCGCATGTCGACATGGAGCGCGACTCGCGCGCCTCATCCATCACCTGCACTGGCCTATTCGC‑GGGTTTACCCGCGAAGA‑GGAAGGCTCAGGCGATATACATCCCCCCTGAC‑AAAAAACCTGT‑ACAACCAACCAg > 7:71092/1‑148 (MQ=255) cGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGGTTAACCGCGAAGC‑GGCCGGCTCAGGCGCATTACATGCCCGCGGAC‑AAAAAAACAGT‑AGAtacaaccaaa > 1:385225/1‑146 (MQ=255) aGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATaa > 3:193955/1‑148 (MQ=255) gCTGGCATCCCGCATGTCTACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGAACTGGCCTCTTCGC‑GGTTTAACCCGCTAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATaaaa < 7:335240/149‑1 (MQ=255) gCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTTATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGTC‑AAAAAACCTGT‑ACATCCATCCAGATaaaa < 1:33236/149‑1 (MQ=255) ggCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGTACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑Acatccatc > 3:113513/1‑137 (MQ=255) aTCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAAACCTGT‑ACATCCATCCAGATAAAACTTGc < 1:47439/149‑1 (MQ=255) ccGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCctct > 5:118731/1‑149 (MQ=255) ccGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCctct > 2:314993/1‑149 (MQ=255) ccGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTAGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATACCCGCGGCA‑AAAAAACCTGT‑AAATCCATCCAGATAAAACTTGCctct > 3:314/1‑149 (MQ=255) ccGCATGTCCACAGGGTCCGCGACTCCCCCGCCTGATCCACCACCTGCACTGCCCTCTTCGC‑GGGTTCACCCGCTAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCctct < 8:94322/149‑1 (MQ=255) tGCGTGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGTCACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTgcg < 1:74951/149‑1 (MQ=255) gcgcGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCa > 7:133240/1‑149 (MQ=255) cTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTAt < 5:181856/149‑1 (MQ=255) ccTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAg < 1:112865/149‑1 (MQ=255) tGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTcccc > 6:87143/1‑149 (MQ=255) gCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCCCCACCCCGATGAAGCGTCCCCgg > 6:202092/1‑149 (MQ=255) gCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCgg > 4:287878/1‑149 (MQ=255) tctTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCa > 2:27236/1‑149 (MQ=255) ttCGC‑GGGTAAACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGccc < 4:116724/98‑1 (MQ=255) ttCGC‑GGGTAAACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGccc > 3:116724/1‑98 (MQ=255) ttCGC‑GGGTAAACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATg > 3:203964/1‑149 (MQ=255) cgc‑gGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGACCACCACCCCGATGAAGCGTCCCCGGCCATgcg > 2:276028/1‑149 (MQ=255) tACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGCCTGttt < 1:27236/148‑1 (MQ=255) | CAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGC‑GGGTTTACCCGCGAAGA‑GGCCGGCTCAGGCGATATACATCCCCGCTGAC‑AAAAAACCTGT‑ACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGCCTGTTT > NC_002947/4529884‑4530173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |