| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,547,609 | G→A | 6.9% | intergenic (‑264/‑80) | pydP ← / → pydB | NCS1 family transporter PydP/bifunctional D‑hydantoinase/dihydropyrimidinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,547,609 | 0 | G | A | 6.9% | 160.6 / 2.0 | 59 | intergenic (‑264/‑80) | pydP/pydB | NCS1 family transporter PydP/bifunctional D‑hydantoinase/dihydropyrimidinase |
| Reads supporting (aligned to +/- strand): ref base G (29/24); new base A (2/2); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.18e-01 | |||||||||||
AAATACCAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCC > NC_002947/4547465‑4547741 | aaaTACCAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGAtt > 3:347456/1‑149 (MQ=255)aaaTACCAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCCTGTCAGTCCTGACAAGAtt > 5:383667/1‑149 (MQ=255) tACCCGGACAACCCCGGCGCCCACTCGCCCGCTAATTTATTCCTATTGATATCAACACCTTAAAAACCACAAATTTAAT‑CGGAAAATTTTCTTGCTGACTCCAGAATCCGCATCTAGCCTCGAATCCTGTCAGTCCTGACAAGAttcga < 8:112826/149‑4 (MQ=255) tACCAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCCTGTCAGTCCTGACAAGAttcga < 6:383667/149‑4 (MQ=255) cAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGAccgc > 2:168418/1‑149 (MQ=255) cAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGAccgc > 7:310375/1‑149 (MQ=255) ggAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGAccgccc > 2:273093/1‑149 (MQ=255) gcaaacCCCCGCCCCCCATCCCCCGGTAAATTTTTCCCAATTTATA‑CAAAAACCTTAAAAACACAAAATTAAA‑TGGCAAATTTTCCTGCCTTATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGAccgccct < 4:360733/147‑1 (MQ=255) cATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATcc < 1:347800/149‑1 (MQ=255) gcgggTTAAATTTTTTCTTTTTATAATAACAACTTTAAAACCACAAATTTTATACATTAAATTAGATATCTTATTCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGa < 1:320723/149‑1 (MQ=255) cgTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAAt < 2:172396/149‑1 (MQ=255) gTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATg > 4:129524/1‑149 (MQ=255) gTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGAACGCCCTG‑CCCGCCCTGCATCCATGACAATg > 4:45556/1‑149 (MQ=255) ttttttCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCGCTGCATCCATGACAATGCa > 2:267906/1‑146 (MQ=255) ccTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCg < 1:273093/148‑1 (MQ=255) cTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCgg < 8:310375/148‑1 (MQ=255) gATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTTTAAGGACTGACATGATTGACCGCCCTG‑CCCGCCCTGCATCAATGACAATGCAAAGAACCGGCCAAGa > 3:209975/1‑149 (MQ=255) atatCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGAc < 6:176439/149‑1 (MQ=255) tatCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGAcc > 7:89894/1‑149 (MQ=255) tCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCg < 2:513/148‑1 (MQ=255) aacaacTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGt > 1:196398/1‑148 (MQ=255) aacaacTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGt < 3:45556/148‑1 (MQ=255) aacaacTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTc < 2:196398/149‑1 (MQ=255) aacaacTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATAAGCATCTACACACGAATCTTGTCAGGACTGACAGGATTGACCGCCCTT‑CACCACCTTCATCCATGACAAACCAAAGAACCGGACAAGACCgga > 1:236673/1‑147 (MQ=255) acaacTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCa < 8:126548/149‑1 (MQ=255) aacTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAg > 8:332272/1‑148 (MQ=255) ttAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTTACAGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTg > 7:143659/1‑149 (MQ=255) ttAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTg > 2:140672/1‑149 (MQ=255) aaaaCCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGc > 6:282399/1‑147 (MQ=255) aCCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAgg > 7:317961/1‑148 (MQ=255) cacaAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCGAAGAACCGGCCAAGACCGGTCAGCCTGCGa > 3:287775/1‑144 (MQ=255) cacaAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑aaa < 4:347456/149‑1 (MQ=255) cacaAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑aa > 7:98400/1‑148 (MQ=255) acaAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑aaaa > 3:232388/1‑149 (MQ=255) aTTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGc < 7:154616/149‑1 (MQ=255) t‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTcc < 7:332272/149‑1 (MQ=255) ggAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGGCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCt > 2:244351/1‑149 (MQ=255) gAAAATTTTCTTGCTGAATCCAGAATCATCATCTAGCCTCGAATCTTGTCAGGACTGACATGATTGACCGCCCTG‑CCCGCCCTGCTTCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCGCt < 2:236673/148‑1 (MQ=255) gAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGACCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTg > 2:250902/1‑149 (MQ=255) ttCTTGCTGAATGCAGAATCAGCATCTAGCCTCGAATGTTGTCAGGACTTACAGGATTGAACGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGACAAGACCGGTCAGCCTGCTAGG‑AAAAGGGCATGTCCCTGTTGATc < 8:143659/148‑1 (MQ=255) ttCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATc < 1:168418/148‑1 (MQ=255) gCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGgc > 5:129588/1‑149 (MQ=255) cTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGgcg > 6:107889/1‑149 (MQ=255) tCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCGTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTa < 1:162048/149‑1 (MQ=255) cAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGTCCCGCCCTGCATCCATGACAATGCAAAGACCCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTa > 1:91539/1‑149 (MQ=255) cAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTCACCGCCCTG‑ACCGCCCTGCATGAATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCACTGTTGATCCGTGCCGCCACCGTGGTTAc > 4:274717/1‑149 (MQ=255) tAGGCTCGAATCTTGTCAGGAATGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGGAAAAGGAAAGAACCGGCCAAGACCGGTCAGCCGGGGAGG‑AAAACGGCATGGGCCGGTTGCTCCGTGGCGCCACCGTGGTTACCCACg > 8:192287/1‑147 (MQ=255) gCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAaga < 4:232388/149‑1 (MQ=255) gCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAaga < 6:129588/149‑1 (MQ=255) gCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAaga < 3:129524/149‑1 (MQ=255) gCCTCGAATCTTGTCACGACTGACAGGTATGACCGCCCAG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGTAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGaa < 1:291339/148‑1 (MQ=255) ccTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCACTGTTGATCCGTGGCGCCACCGTGGTTACCCACGaa > 4:184285/1‑146 (MQ=255) cTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAagaga < 3:84705/149‑1 (MQ=255) cTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAagaga < 2:328851/149‑1 (MQ=255) cTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAaga > 3:264986/1‑147 (MQ=255) tCGAATCTTGTCAGGACTGACAGGATTAACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAagagag > 2:44579/1‑149 (MQ=255) gtcaaTCCTGTCAGTCCTGACAAGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAagagag > 4:344517/4‑149 (MQ=255) gAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGt > 5:299004/1‑148 (MQ=255) gTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTAccc < 8:89894/146‑1 (MQ=255) | AAATACCAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAT‑GGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTG‑CCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGG‑AAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCC > NC_002947/4547465‑4547741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |