Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,580,603 T→A 6.4% K942M (AAG→ATG) ‡ treSB ← bifunctional trehalose synthase B/maltokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,580,6030TA 6.4% 187.2 / 3.0 62K942M (AAG→ATG) ‡treSBbifunctional trehalose synthase B/maltokinase
Reads supporting (aligned to +/- strand):  ref base T (27/31);  new base A (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

AAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATC  >  NC_002947/4580457‑4580740
                                                                                                                                                   |                                                                                                                                          
aaGGGCTGTGTTTTGCGCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACTCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCtgct‑t                                                                                                                                          >  1:286625/1‑149 (MQ=255)
  gggCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCtgct‑tgct                                                                                                                                       >  1:216093/1‑149 (MQ=255)
    gCTGTGTTTTGCGCGGCGCTCTTCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCa                                                                                                                                     <  2:286625/149‑1 (MQ=255)
     cTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAg                                                                                                                                    <  1:169229/149‑1 (MQ=255)
      tgtgGTTTGCCCGACGCTCTTCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAgg                                                                                                                                   <  2:287551/149‑1 (MQ=255)
       ttgtTTTGCCCGGCTCTCTTCAAGGGGACGGTCGGGTTCAC‑CTTCAAAATCGATTAGGTAGGCATCACCCTGCACAACGAGCACCTGGCCCAGGTGCAGGTCTCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCA‑GGCTCAgg                                                                                                                                   <  4:87981/147‑1 (MQ=255)
        tgtTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGgt                                                                                                                                 <  1:63691/149‑1 (MQ=255)
        tgtTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCA‑GGCTCAggg                                                                                                                                  <  3:309710/148‑1 (MQ=255)
        ggtTTTGCCCGGCGCTCTTCAAGGTGCCGGGCGGGTTCAC‑CTTCAATATCGTTGAGGTAGGCATCACCCTGCACGACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTAGACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGgt                                                                                                                                 <  4:91626/148‑1 (MQ=255)
               cccGGCGCTCTTCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGAtg                                                                                                                          >  5:12591/1‑149 (MQ=255)
                 cGGCGCTCTTCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCACAGGGTGTCGAtgtg                                                                                                                        >  7:344993/1‑149 (MQ=255)
                  ggCGCTCTTCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGc                                                                                                                       <  3:177977/149‑1 (MQ=255)
                         ttCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGc                                                                                                                  <  2:329276/147‑1 (MQ=255)
                           cAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGcc                                                                                                                 <  2:234490/146‑1 (MQ=255)
                           cAAGGGGCCGGGCGGGTTCAA‑CTTCAAAATCGATGAGGTAAGAATCACCCTGAACCACCAGCACCTGTCCCAGGTGAAGGTCCCCGAGTACACGCATAAGCAGGCCACCCTCAGCCTCCT‑TGCTCAGGATGTCGATGTGCTGGGCCAgg                                                                                                              >  6:150518/1‑149 (MQ=255)
                                          gTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTATGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTtg                                                                                                <  1:271056/149‑1 (MQ=255)
                                          gTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTtgc                                                                                               >  8:230378/1‑149 (MQ=255)
                                                 cTTCATAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCAGCGTGAGCCTGCT‑TGCTGAGGGTGTCGATGTGCTGGGCCAGTCCGTCACGCTGTTGCTGCAgg                                                                                         <  5:150518/149‑1 (MQ=255)
                                                      aaaTCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGgtcgtc                                                                                    >  4:261509/1‑149 (MQ=255)
                                                      aaaTCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGgtcgtc                                                                                    <  6:12591/149‑1 (MQ=255)
                                                       aaTCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGgtcgtc                                                                                    >  3:54541/1‑148 (MQ=255)
                                                           gATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGAcc                                                                                <  2:216093/148‑1 (MQ=255)
                                                             tGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAg                                                                             <  7:230378/149‑1 (MQ=255)
                                                                  tAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTg                                                                        <  7:279479/149‑1 (MQ=255)
                                                                   aGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTgg                                                                       >  6:68870/1‑149 (MQ=255)
                                                                      cATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTa                                                                    >  6:188476/1‑149 (MQ=255)
                                                                       aTCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTAt                                                                   >  5:376930/1‑149 (MQ=255)
                                                                       aTCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGAGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTAt                                                                   >  1:340696/1‑149 (MQ=255)
                                                                        tCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCTAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATc                                                                  <  6:346903/149‑1 (MQ=255)
                                                                            ccTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCg                                                                 >  1:290203/1‑146 (MQ=255)
                                                                            ccTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCg                                                                 <  2:290203/146‑1 (MQ=255)
                                                                                 accacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAgg                                                                                         <  1:65816/117‑1 (MQ=255)
                                                                                 accacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAgg                                                                                         >  2:65816/1‑117 (MQ=255)
                                                                                   cacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAg                                                                             <  1:241720/127‑1 (MQ=255)
                                                                                   cacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAg                                                                             >  2:241720/1‑127 (MQ=255)
                                                                                      ctgCACCTGGGCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAgtgtgt                                                    >  3:376997/3‑149 (MQ=255)
                                                                                         cACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTcgc                                                 <  5:68870/149‑1 (MQ=255)
                                                                                            cTGGCCCAGGTGCAGGTCGCCGTGTACAGGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAgt                                                        <  5:278458/139‑1 (MQ=255)
                                                                                            cTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGAt                                              >  4:10593/1‑149 (MQ=255)
                                                                                            cTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGAt                                              >  2:323330/1‑149 (MQ=255)
                                                                                            cTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTAACGCTGTTGCTGAAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCTgt                                                        >  6:278458/1‑139 (MQ=255)
                                                                                                 ccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTgg                                         >  4:241088/1‑149 (MQ=255)
                                                                                                 ccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTgg                                         <  4:232844/149‑1 (MQ=255)
                                                                                                 ccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGGGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGCCCAAGGCCTGGCTAGCGCTGTCCAGTGTGTCGCGATGCTgg                                         >  4:249145/1‑149 (MQ=255)
                                                                                                       gCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCcag                                    >  8:103796/1‑148 (MQ=255)
                                                                                                        cAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCcagca                                  <  3:350118/149‑1 (MQ=255)
                                                                                                         aGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCcagcag                                 <  4:99604/149‑1 (MQ=255)
                                                                                                               ccGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAg                           >  4:102726/1‑149 (MQ=255)
                                                                                                               ccGTCTACACGCATAAGCAGGCCACCCTCAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAg                           >  6:338350/1‑149 (MQ=255)
                                                                                                                      cacGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTgg                    <  4:376997/149‑1 (MQ=255)
                                                                                                                      cacGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTgg                    <  4:54541/149‑1 (MQ=255)
                                                                                                                      cacGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTgg                    <  3:10593/149‑1 (MQ=255)
                                                                                                                      cacGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTgg                    >  7:341305/1‑149 (MQ=255)
                                                                                                                       acGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTggg                   <  7:103796/149‑1 (MQ=255)
                                                                                                                        cGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGt                  <  6:376930/149‑1 (MQ=255)
                                                                                                                         gCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTg                                          <  6:5551/124‑1 (MQ=255)
                                                                                                                         gCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGt                  >  8:32967/1‑148 (MQ=255)
                                                                                                                           aTAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcag               >  3:18156/1‑149 (MQ=255)
                                                                                                                             aaGCAGGCCACCCTGAGCCTGCT‑TGCTCAGAGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagtt             <  2:340696/149‑1 (MQ=255)
                                                                                                                                cAGGCCACCGTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagttcag          <  5:338350/149‑1 (MQ=255)
                                                                                                                                  ggCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTg        >  2:107499/1‑149 (MQ=255)
                                                                                                                                   gCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGc       >  4:100268/1‑149 (MQ=255)
                                                                                                                                    acACCCTGAGCAAGCA‑GGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCt      >  8:90332/2‑149 (MQ=255)
                                                                                                                                        ccTGAGCAAGCA‑GGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATc  >  3:72975/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
AAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCAC‑CTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCT‑TGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATC  >  NC_002947/4580457‑4580740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑