Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,604,205	C→G	9.2%	P12R (CCG→CGG) ‡	PP_4076 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,604,205	0	C	G	9.2%	226.5 / 12.8	76	P12R (CCG→CGG) ‡	PP_4076	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (33/36);  new base G (5/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01

CTCGGACGTGGCGGGTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGCATGA  >  NC_002947/4604057‑4604352                                                                                                                                                     |                                                                                                                                                    ctcGGACGTGGCGGGTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGAccc                                                                                                                                                     >  6:84311/1‑149 (MQ=255)  tcGGACGTGGCGGGTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCg                                                                                                                                                    >  1:263883/1‑149 (MQ=255)  tcGGACGTGGCGGGTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCg                                                                                                                                                    >  7:217057/1‑149 (MQ=255)   cGGACGTGGCGGGTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGt                                                                                                                                                   >  4:197918/1‑149 (MQ=255)    ggACGTGGCGGGTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTc                                                                                                                                                  <  6:329477/149‑1 (MQ=255)            cGGGTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATcc                                                                                                                                          <  2:263883/149‑1 (MQ=255)             gggTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCg                                                                                                                                         >  1:70576/1‑149 (MQ=255)             gggTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCg                                                                                                                                         >  5:246452/1‑149 (MQ=255)              ggTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACGGGTCCGCCATcaag                                                                                                                                        <  4:355027/149‑4 (MQ=255)              ggTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACGGGTCCGCCATcaag                                                                                                                                        <  3:117205/149‑4 (MQ=255)               gTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCggg                                                                                                                                       <  6:34781/149‑1 (MQ=255)                tGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGa                                                                                                                                      <  4:317150/149‑1 (MQ=255)                   tCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGc                                                                                                                                   >  4:378601/1‑149 (MQ=255)                      gggCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGt                                                                                                                                 >  8:89599/1‑148 (MQ=255)                        gCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGtctc                                                                                                                              <  7:218525/149‑1 (MQ=255)                            gTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGGCATCCGGGAGGCGTCgcgc                                                                                                                            >  8:97174/1‑147 (MQ=255)                            gTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCa                                                                                                                          <  3:261062/149‑1 (MQ=255)                                 aGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTTCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGaaa                                                                                                                     <  7:25555/149‑1 (MQ=255)                                        gctcgcCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCtt                                                                                                               <  5:118101/148‑1 (MQ=255)                                        gctcgcCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTc                                                                                                              <  5:174590/149‑1 (MQ=255)                                         ctcgcCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCg                                                                                                             >  3:7300/1‑149 (MQ=255)                                          tcgcCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCg                                                                                                             >  4:87889/1‑148 (MQ=255)                                           cgcCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGca                                                                                                           <  5:73142/149‑1 (MQ=255)                                              cGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGcac                                                                                                          >  5:185391/1‑147 (MQ=255)                                                     ccGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTcgcg                                                                                                 <  5:264010/149‑1 (MQ=255)                                                       gCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGc                                                                                               >  7:245121/1‑149 (MQ=255)                                                        cACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGcc                                                                                              <  8:231576/149‑1 (MQ=255)                                                          cTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCAt                                                                                            >  7:4980/1‑149 (MQ=255)                                                             cAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATc                                                                                           <  3:87889/147‑1 (MQ=255)                                                              aaGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAg                                                                                        >  2:115530/1‑149 (MQ=255)                                                              aaGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAg                                                                                        >  2:143142/1‑149 (MQ=255)                                                                gTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTg                                                                                      >  7:230780/1‑149 (MQ=255)                                                                 tACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGc                                                                                     <  2:329716/149‑1 (MQ=255)                                                                   cATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGcc                                                                                    <  8:230780/148‑1 (MQ=255)                                                                    aTGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCAc                                                                                  <  3:316872/149‑1 (MQ=255)                                                                     tGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCa                                                                                                                          <  4:35782/108‑1 (MQ=255)                                                                     tGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCa                                                                                                                          >  3:35782/1‑108 (MQ=255)                                                                        gcgcGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTc                                                                               >  7:122245/1‑148 (MQ=255)                                                                          gcgAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGa                                                                             >  7:157765/1‑148 (MQ=255)                                                                           cgAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGaaa                                                                           <  4:23221/149‑1 (MQ=255)                                                                                ttCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTg                                                                      >  6:324230/1‑149 (MQ=255)                                                                                ttCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGAACCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTg                                                                      >  5:297263/1‑149 (MQ=255)                                                                                  cGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGc                                                                     <  8:217057/148‑1 (MQ=255)                                                                                   gAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCt                                                                    <  5:90179/148‑1 (MQ=255)                                                                                   gAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCt                                                                    >  1:57453/1‑148 (MQ=255)                                                                                     aaTTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGaa                                                                 <  5:84311/149‑1 (MQ=255)                                                                                      aTTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAAc                                                                <  5:229573/149‑1 (MQ=255)                                                                                           ccTTGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTc                                                            <  7:89599/148‑1 (MQ=255)                                                                                            cTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCAACAAGTGCCACCTCGAAACGTTGCTGAACGCTCGc                                                          >  7:179711/1‑149 (MQ=255)                                                                                                     cGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGc                                                 >  8:322415/1‑149 (MQ=255)                                                                                                            tcacGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCaga                                          <  5:199401/148‑1 (MQ=255)                                                                                                            acacGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCTGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCaga                                          >  7:136629/1‑149 (MQ=255)                                                                                                            acacGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGGTGCTCCCaga                                          <  6:185391/149‑1 (MQ=255)                                                                                                              acGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCaga                                          >  6:199401/1‑147 (MQ=255)                                                                                                                           tttCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTgg                           <  5:324230/149‑1 (MQ=255)                                                                                                                           tttCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTgg                           <  1:115530/149‑1 (MQ=255)                                                                                                                             tcttcGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGc                         >  7:62433/1‑149 (MQ=255)                                                                                                                              cttcGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGACACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGc                         <  3:197918/148‑1 (MQ=255)                                                                                                                              cttcGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCGCCGAGCTGGGc                         <  8:62433/148‑1 (MQ=255)                                                                                                                               ttcGATCGCTTGATGGCGGACCCGTCCGGCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCt                       >  1:217325/1‑149 (MQ=255)                                                                                                                               ttcGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTTCCACCTCGAAACGTTGCTGAACGCTCGCCAAGCTTGCTCCCAGAGCAGCCCCGAGCTGGGCCt                       >  5:321434/1‑149 (MQ=255)                                                                                                                                  gATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTgcg                    >  6:371258/1‑149 (MQ=255)                                                                                                                                   aTCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTgcgc                   <  8:157765/149‑1 (MQ=255)                                                                                                                                     tcccggATGGCGGACGGGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCTACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGa                 >  8:313577/6‑149 (MQ=255)                                                                                                                                      gCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGa                 <  2:70576/148‑1 (MQ=255)                                                                                                                                      gCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGa                 <  8:245121/148‑1 (MQ=255)                                                                                                                                      gCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGAc                >  8:364773/1‑149 (MQ=255)                                                                                                                                       ccggATGGCGGACGGGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACt               >  8:116011/4‑149 (MQ=255)                                                                                                                                       cTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACt               <  8:136629/149‑1 (MQ=255)                                                                                                                                        ttGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACt               >  3:227356/1‑148 (MQ=255)                                                                                                                                         ggATGGCGGACGGGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACtt              >  6:150293/2‑148 (MQ=255)                                                                                                                                          gATGGCGGACGGGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCAGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTc             >  4:75733/1‑148 (MQ=255)                                                                                                                                          gATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACtt              <  8:4980/147‑1 (MQ=255)                                                                                                                                           aTGGCGGACGGGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCaa           >  8:260390/1‑149 (MQ=255)                                                                                                                                              gCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACgg        >  5:322568/1‑149 (MQ=255)                                                                                                                                                ggACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGc      <  2:217325/149‑1 (MQ=255)                                                                                                                                                ggACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGc      <  7:322415/149‑1 (MQ=255)                                                                                                                                                    ccGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGCAt    <  2:380676/147‑1 (MQ=255)                                                                                                                                                    ccGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGCAt    >  1:380676/1‑147 (MQ=255)                                                                                                                                                    ccGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGCATGa  >  1:174857/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CTCGGACGTGGCGGGTGATCCGGGCTGGTACCAGGTCTCGCTCGCCGTGCGCCCGCACTTCAAGTACATGGGCGCGAACTTCGAAATTTCCCTGGTAGGCCGGCTCGACACGCAATGAGTGGTTTCTTCGATCGCTTGATGGCGGACCCGTCCGCCATCCGGGAGGCGTCTCGCCAGGAAAGCGCTTCGCACAAGTTCGCGGCCATCAAGTGCCACCTCGAAACGTTGCTGAACGCTCGCCAAGGTTGCTCCCAGAGCAGCCCCGAGCTGGGCCTGCGCGACTTCAACGGGCATGA  >  NC_002947/4604057‑4604352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑