Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,634,500	T→C	5.3%	Q204R (CAA→CGA)	uvrC ←	UvrABC system protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,634,500	0	T	C	5.3%	223.9 / 2.0	75	Q204R (CAA→CGA)	uvrC	UvrABC system protein C
Reads supporting (aligned to +/- strand):  ref base T (31/40);  new base C (2/2);  total (33/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

GTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGCCGGGTGCGGTTGGCGTAGTAGCTGTC  >  NC_002947/4634355‑4634645                                                                                                                                                  |                                                                                                                                                    gTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCt                                                                                                                                                  >  3:97442/1‑149 (MQ=255) gTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCt                                                                                                                                                  >  8:153443/1‑149 (MQ=255) gTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCt                                                                                                                                                  >  4:98182/1‑149 (MQ=255)   cGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTgg                                                                                                                                                >  3:142923/1‑149 (MQ=255)   cGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTCGCTgg                                                                                                                                                >  1:114086/1‑149 (MQ=255)   cGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTCGCTgg                                                                                                                                                >  1:119037/1‑149 (MQ=255)    gACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTgg                                                                                                                                                <  1:53539/148‑1 (MQ=255)          gccgcTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGcc                                                                                                                                         >  8:218726/1‑149 (MQ=255)          gccgcTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGc                                                                                                                                          >  6:308749/1‑148 (MQ=255)          gccgcTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCAATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGcc                                                                                                                                         >  6:303482/1‑149 (MQ=255)             gcTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCtt                                                                                                                                      >  1:321879/1‑149 (MQ=255)              cTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTc                                                                                                                                     <  3:120001/149‑1 (MQ=255)               tGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTa                                                                                                                                             <  2:110900/140‑1 (MQ=255)               tGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAACTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTa                                                                                                                                             >  1:110900/1‑140 (MQ=255)                 ccgccCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTcc                                                                                                                                    <  1:345667/147‑1 (MQ=255)                 ccgccCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTcc                                                                                                                                    <  1:333080/147‑1 (MQ=255)                 ccgccCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCa                                                                                                                                   <  3:98182/148‑1 (MQ=255)                 ccgccCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAg                                                                                                                                  <  5:303482/149‑1 (MQ=255)                 ccgccCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAg                                                                                                                                  <  5:308749/149‑1 (MQ=255)                  cgccCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGa                                                                                                                                 <  7:84305/149‑1 (MQ=255)                    cccTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGaaa                                                                                                                               <  2:321879/149‑1 (MQ=255)                           tGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCAcc                                                                                                                        <  1:317173/149‑1 (MQ=255)                             tACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCg                                                                                                                       >  6:9126/1‑148 (MQ=255)                               cTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGaa                                                                                                                     <  4:371295/148‑1 (MQ=255)                                tGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAAt                                                                                                                    >  2:106072/1‑148 (MQ=255)                                tGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTAGCTACGCCCTTCCAGAAACATCACCGAAt                                                                                                                    >  2:338967/1‑148 (MQ=255)                                  ttGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGAAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCAATCTCGGCGTTCAACTCATAGAACAGTTGCTGGCTAAGCCCTTCCAGAAACATCACCGAATgta                                                                                                                 >  4:287400/1‑147 (MQ=255)                                    ggTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACg                                                                                                                >  2:74285/1‑148 (MQ=255)                                        cTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCCTCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGt                                                                                                           >  2:333225/1‑149 (MQ=255)                                         tGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTc                                                                                                          >  1:228732/1‑149 (MQ=255)                                               cggcgCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGc                                                                                                   <  2:258662/149‑1 (MQ=255)                                                   gcAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGc                                                                                                                                          >  7:86281/1‑107 (MQ=255)                                                   gcAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGc                                                                                                                                          <  8:86281/107‑1 (MQ=255)                                                   gcAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAt                                                                                               >  3:211314/1‑149 (MQ=255)                                                   gcAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAt                                                                                               <  2:151797/149‑1 (MQ=255)                                                     aacaaGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑Tc                                                                                            <  7:187338/149‑1 (MQ=255)                                                     aacaaGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑Tc                                                                                            <  7:218726/149‑1 (MQ=255)                                                        aaGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGTCGAACGTCCTC‑TGCGTAT‑TCCTc                                                                                         <  3:287400/149‑1 (MQ=255)                                                         aGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCg                                                                                        <  4:142923/149‑1 (MQ=255)                                                         aGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCg                                                                                        <  1:304049/149‑1 (MQ=255)                                                          ggagATCTGGTCGCGCAGCTCGTCGGCGATTGCGAAATCGAGTGCCAGGGCGGCCTTTTCCATCTCGGCGCTAAACTCCTTGCCCAGTTTCTGGCTACGCCCTTCCAGACACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCgg                                                                                       <  4:257521/146‑1 (MQ=255)                                                           gCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCATTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCgg                                                                                       >  1:102422/1‑148 (MQ=255)                                                             gATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAg                                                                                    >  5:324886/1‑149 (MQ=255)                                                               tCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCGCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGt                                                                                   >  4:98006/1‑148 (MQ=255)                                                               tCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGTCAGt                                                                                   >  6:157330/1‑148 (MQ=255)                                                                   gTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCAcc                                                                               <  4:97442/148‑1 (MQ=255)                                                                       cgcAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGTCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTc                                                                          <  5:9126/149‑1 (MQ=255)                                                                        gcAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCg                                                                         >  6:175636/1‑149 (MQ=255)                                                                          aGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGt                                                                       <  4:286524/149‑1 (MQ=255)                                                                          aGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTCCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGt                                                                       >  3:45487/1‑149 (MQ=255)                                                                                     tttttCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCtt                                                            >  6:114835/1‑149 (MQ=255)                                                                                       tttCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTAGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCtt                                                            <  1:338967/147‑1 (MQ=255)                                                                                               tCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTATCTCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGtt                                                   <  1:74285/149‑1 (MQ=255)                                                                                               tCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTCCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGttt                                                  <  4:45487/149‑1 (MQ=255)                                                                                                 gAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTa                                                >  8:73813/1‑149 (MQ=255)                                                                                                    ggCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCt                                             <  7:73813/149‑1 (MQ=255)                                                                                                     gCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTg                                            <  2:102422/149‑1 (MQ=255)                                                                                                       cATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACCCACGTCCTCTTTCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTgg                                           <  1:106072/149‑1 (MQ=255)                                                                                                        aTGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTa                                         <  2:228732/149‑1 (MQ=255)                                                                                                              gcCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTCGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAg                                   <  2:119037/149‑1 (MQ=255)                                                                                                              gcCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTCGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAg                                   <  2:114086/149‑1 (MQ=255)                                                                                                                cTTTTCCATCTAGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGc                                 <  5:157330/149‑1 (MQ=255)                                                                                                                   ttCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCAt                               <  5:114835/148‑1 (MQ=255)                                                                                                                     ccATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATgg                             <  3:98006/148‑1 (MQ=255)                                                                                                                      cagctcGGCGTTCAACTCCTTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGACTGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGc                            <  6:87651/145‑1 (MQ=255)                                                                                                                         ctcGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGCCggg                        >  8:15667/1‑149 (MQ=255)                                                                                                                                        aTTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGCCGGGTGCGGTTGGCgtag          >  5:182547/1‑148 (MQ=255)                                                                                                                                         ttGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGCCgg                         <  6:146810/132‑1 (MQ=255)                                                                                                                                         ttGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGCCgg                         >  5:146810/1‑132 (MQ=255)                                                                                                                                          tGCCCAGTTGCTGGCTACGCCCTTCCAGAACCATCACCGAATGCCGACCGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGGTTTATCTGGTACTGCAGGCATGGCCGGGGGCGGTTGGCgtagt         <  7:64461/147‑1 (MQ=255)                                                                                                                                          tGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGCCGGGTGCGGTTGGCgtagg         >  8:64461/1‑146 (MQ=255)                                                                                                                                          cacccAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGCCGGGTGCGGTTGGCGtagtag       <  1:333225/147‑1 (MQ=255)                                                                                                                                           gCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGCCGGGTGCGGTTGGCGtagtag       <  7:15667/148‑1 (MQ=255)                                                                                                                                            cccAGTTGCTGGCTACGCCCTTCCAGAATCCTCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGCCGGGTGCGGTTGGCGTAGTAGCt     <  2:283835/149‑1 (MQ=255)                                                                                                                                               aGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGCCGGGTGCGGTTGGCGTAGTAGCTGTc  >  3:35908/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGCTACGCCCTTCCAGAAACATCACCGAATGACGAACGTCCTC‑TGCGTAT‑TCCTCGGCAGTCACCAGGTCGGTGCAGGGCCCTTTGCAGCGTTTTATCTGGTACTGCAGGCATGGCCGGGTGCGGTTGGCGTAGTAGCTGTC  >  NC_002947/4634355‑4634645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑