Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,687,380 T→C 7.5% L119P (CTG→CCG)  PP_4148 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,687,3800TC 7.5% 230.3 / 5.9 79L119P (CTG→CCG) PP_4148ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (36/37);  new base C (3/3);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGGCCTGGACCCGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCTCGGCAATCATCGTCGGC  >  NC_002947/4687232‑4687528
                                                                                                                                                    |                                                                                                                                                    
cAGGGCCTGGACCCGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCt                                                                                                                                                      >  6:53187/1‑149 (MQ=255)
cAGGGCCTGGACCCGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCt                                                                                                                                                      <  7:337912/149‑1 (MQ=255)
 aGGGCCTGGACCCGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTg                                                                                                                                                     <  2:121810/149‑1 (MQ=255)
 aGGGCCTGGACCCGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTg                                                                                                                                                     <  7:357242/149‑1 (MQ=255)
  gggCCTGGACCCGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGa                                                                                                                                                    <  3:381433/149‑1 (MQ=255)
    gCCTGGACCCGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATc                                                                                                                                                  >  8:48679/1‑149 (MQ=255)
         gACCCGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGcc                                                                                                                                               >  5:204371/1‑147 (MQ=255)
            ccGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCgag                                                                                                                                            >  8:330467/1‑147 (MQ=255)
            ccGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGagaa                                                                                                                                          >  3:312581/1‑149 (MQ=255)
             cGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCgag                                                                                                                                            >  7:74255/1‑146 (MQ=255)
                cgcTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATg                                                                                                                                      <  6:190506/149‑1 (MQ=255)
                    gATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCgg                                                                                                                                  >  3:262669/1‑149 (MQ=255)
                        gCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGttt                                                                                                                               <  3:123540/148‑1 (MQ=255)
                        gCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTc                                                                                                                              >  4:175485/1‑149 (MQ=255)
                         cTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCg                                                                                                                             <  8:17931/149‑1 (MQ=255)
                              aTCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCAACCTGATCGCCGAGAAGATGCCGGTTTCGAtctc                                                                                                                        >  3:277889/1‑149 (MQ=255)
                               tCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCg                                                                                                                       >  2:221953/1‑149 (MQ=255)
                                 gAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGttt                                                                                                                               >  7:96992/1‑139 (MQ=255)
                                 gAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGttt                                                                                                                               <  8:96992/139‑1 (MQ=255)
                                          tACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATg                                                                                                            <  7:330467/149‑1 (MQ=255)
                                           aCGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCTCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATg                                                                                                            <  4:312581/148‑1 (MQ=255)
                                           aCGGCTTCGAAAAGTCGCCGCCCGAGCGCCTGTGTATCATGGTAAAGAACTACGCCCTGCTGTACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCGTATAATTTGCCGGTTTCGATCTCGCTGGGGCTATg                                                                                                            <  1:221953/148‑1 (MQ=255)
                                            cGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGa                                                                                                          <  4:262669/149‑1 (MQ=255)
                                              gCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAg                                                                                                         >  6:220050/1‑148 (MQ=255)
                                               cTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCa                                                                                                       >  5:374651/1‑149 (MQ=255)
                                                ttCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCAc                                                                                                      >  3:340341/1‑149 (MQ=255)
                                                   gACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGc                                                                                                    <  5:220050/148‑1 (MQ=255)
                                                   gACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGc                                                                                                    <  6:374651/148‑1 (MQ=255)
                                                    aCAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCt                                                                                                   >  3:86810/1‑148 (MQ=255)
                                                     cAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGa                                                                                                 >  1:100295/1‑149 (MQ=255)
                                                      aaGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGttt                                                                                                                               <  7:67606/118‑1 (MQ=255)
                                                      aaGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGttt                                                                                                                               >  8:67606/1‑118 (MQ=255)
                                                      aaGTCGCCGCACGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCATCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTTGGGCTATGGAGCACGCTGa                                                                                                 >  4:324837/1‑148 (MQ=255)
                                                        gTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCa                                                                                              <  8:74255/149‑1 (MQ=255)
                                                           gccgccCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATg                                                                                            >  6:218970/1‑148 (MQ=255)
                                                            ccgccCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTa                                                                                          <  5:53187/149‑1 (MQ=255)
                                                            ccgccCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCACTGGGGCGATGGAGCACGCTGATCATGTa                                                                                          >  4:97927/1‑149 (MQ=255)
                                                               cccGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCt                                                                                       >  4:94262/1‑149 (MQ=255)
                                                                ccGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTg                                                                                      <  7:177563/149‑1 (MQ=255)
                                                                ccGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTg                                                                                      <  3:175485/149‑1 (MQ=255)
                                                                ccGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCGTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTg                                                                                      <  5:317321/149‑1 (MQ=255)
                                                                     cgcCTGTGGATCATGGTCAACAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTc                                                                                 <  8:98355/149‑1 (MQ=255)
                                                                        cTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGAt                                                                              <  6:204371/149‑1 (MQ=255)
                                                                        cTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGAt                                                                              >  7:105994/1‑149 (MQ=255)
                                                                          gtgGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATcc                                                                            >  6:246922/1‑149 (MQ=255)
                                                                           tgGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATc                                                                             <  7:48679/147‑1 (MQ=255)
                                                                           tgGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCAACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATc                                                                             <  4:277889/147‑1 (MQ=255)
                                                                             gATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGc                                                                         >  4:80508/1‑149 (MQ=255)
                                                                               tCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTg                                                                       <  7:177437/149‑1 (MQ=255)
                                                                                    gTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGa                                                                   <  4:334583/148‑1 (MQ=255)
                                                                                       aaGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGc                                                               <  4:86810/149‑1 (MQ=255)
                                                                                        aGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGcc                                                              <  5:218970/149‑1 (MQ=255)
                                                                                            cTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCGGCTGGGGATCGCCAAgg                                                          <  3:80508/149‑1 (MQ=255)
                                                                                             tACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGc                                                         >  7:237499/1‑149 (MQ=255)
                                                                                               cGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCg                                                       <  5:246922/149‑1 (MQ=255)
                                                                                                  ccAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTgc                                                    >  2:293558/1‑149 (MQ=255)
                                                                                                  ccAGCTGGACTTCGGCGACAGCTTCTGCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTgc                                                    >  1:10249/1‑149 (MQ=255)
                                                                                                      cTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCa                                                >  7:225912/1‑149 (MQ=255)
                                                                                                        ggACTTCGGCGACAGCTTCTTCCGCGACGCCAACGTGATCGACCT‑ATCGCCGATAATATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACATGGTTTCTATCCCCCTCGGGATCGCCAAGGCCGTgcgcaaagg                                             >  7:314045/1‑144 (MQ=255)
                                                                                                         gACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCAc                                               <  8:105994/147‑1 (MQ=255)
                                                                                                            ttCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAg                                          >  7:338287/1‑149 (MQ=255)
                                                                                                            ttCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATAGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTCATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAg                                          >  4:47435/1‑149 (MQ=255)
                                                                                                                        ttcttcCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACt                                     <  7:120861/142‑1 (MQ=255)
                                                                                                                        ttcttcCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACt                                     >  8:120861/1‑142 (MQ=255)
                                                                                                                        ttcttcCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACgt                              >  7:288397/1‑149 (MQ=255)
                                                                                                                             ccGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCaa                                                            <  1:192056/114‑1 (MQ=255)
                                                                                                                             ccGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCaa                                                            >  2:192056/1‑114 (MQ=255)
                                                                                                                             ccGCGACGCCAAGGTGATCGACCCGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGa                         >  1:86629/1‑149 (MQ=255)
                                                                                                                             ccGCGACGCCAAGGTGATCGACCCGATCGCCGAGAAGATGCCGGTGTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGa                         >  1:81252/1‑149 (MQ=255)
                                                                                                                                cgACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCa                      <  3:340805/149‑1 (MQ=255)
                                                                                                                                     ccAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCTCg                 >  1:98455/1‑149 (MQ=255)
                                                                                                                                      cAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCt                   <  8:102542/146‑1 (MQ=255)
                                                                                                                                      cAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCt                   >  7:102542/1‑146 (MQ=255)
                                                                                                                                      cAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCTCgg                <  8:338287/149‑1 (MQ=255)
                                                                                                                                      cAAGGTGATCGACCCGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCTCgg                <  2:81252/149‑1 (MQ=255)
                                                                                                                                      cAAGGTGATCGACCCGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCTCgg                <  2:86629/149‑1 (MQ=255)
                                                                                                                                       aaGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCTCGGc               <  1:293558/149‑1 (MQ=255)
                                                                                                                                                 aCCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCTCGGCAATCAtcgtc     >  3:204735/1‑149 (MQ=255)
                                                                                                                                                 aCCCGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGc                    >  5:179813/1‑134 (MQ=255)
                                                                                                                                                 aCCCGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGc                    <  6:179813/134‑1 (MQ=255)
                                                                                                                                                  ccTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCTCGGCAATCAtcgtcg    <  3:94262/149‑1 (MQ=255)
                                                                                                                                                    tGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCTCGGCAATCATCGTCGGc  >  4:294594/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGGGCCTGGACCCGGCGCTGATCGCTGAAATCGAACGCATGTACGGCTTCGACAAGTCGCCGCCCGAGCGCCTGTGGATCATGGTCAAGAACTACGCCCAGCTGGACTTCGGCGACAGCTTCTTCCGCGACGCCAAGGTGATCGACCTGATCGCCGAGAAGATGCCGGTTTCGATCTCGCTGGGGCTATGGAGCACGCTGATCATGTACCTGGTGTCGATCCCGCTGGGGATCGCCAAGGCCGTGCGCCACGGCAGCCACTTCGACGTGTGGACCAGCTCGGCAATCATCGTCGGC  >  NC_002947/4687232‑4687528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑