Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,711,531 T→G 7.1% Q4P (CAG→CCG)  gpsA ← glycerol‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,711,5310TG 7.1% 168.9 / 4.5 56Q4P (CAG→CCG) gpsAglycerol‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (22/30);  new base G (2/2);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

AGATAGCGCGGATTCTCGCGATTCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGC  >  NC_002947/4711384‑4711676
                                                                                                                                                   |                                                                                                                                                 
agaTAGCGCGGATTCTCGCGATTCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACGGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTg                                                                                                                                                  <  8:96864/149‑1 (MQ=255)
 gaTAGCGCGGATTCTCGCGATTCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGt                                                                                                                                                 <  2:301369/149‑1 (MQ=255)
  aTAGCGCGGATTCTCGCGATTCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGtt                                                                                                                                                <  1:250292/149‑1 (MQ=255)
      cgcgGATTCTCGCGATTCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACAGCAACCTGTTGCGGTTctgg                                                                                                                                            <  3:241696/149‑4 (MQ=255)
        cgGATTCTCGCGATTCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCa                                                                                                                                          >  5:338891/1‑149 (MQ=255)
         ggATTCTCGCGATTCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCa                                                                                                                                          >  7:165103/1‑148 (MQ=255)
         ggATTCTCGCGATTCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACAGCAACCTGTTGCGGTTctgggcg                                                                                                                                         <  6:393854/149‑7 (MQ=255)
               tcGCGATTCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATatccaa                                                                                                                                   >  5:214055/1‑149 (MQ=255)
                 gcgATTCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATatccaatc                                                                                                                                 <  1:302137/149‑1 (MQ=255)
                      tCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCAtt                                                                                                                             >  5:349742/1‑148 (MQ=255)
                       cacaCGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTa                                                                                                                            <  7:24405/148‑1 (MQ=255)
                        acacGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTaaa                                                                                                                          <  1:326790/149‑1 (MQ=255)
                         cacGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTaaa                                                                                                                          <  1:260991/148‑1 (MQ=255)
                         cacGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTa                                                                                                                            >  2:260991/1‑146 (MQ=255)
                          acGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATatccaat                                                                                                                                  >  5:202361/1‑139 (MQ=255)
                          acGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATatccaat                                                                                                                                  <  6:202361/139‑1 (MQ=255)
                          acGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGc                                                                                                                        >  1:238677/1‑149 (MQ=255)
                            gCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGcc                                                                                                                       >  7:360339/1‑148 (MQ=255)
                              aTGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCaa                                                                                                                     >  7:21867/1‑148 (MQ=255)
                               tGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCaaaa                                                                                                                   <  5:381120/149‑1 (MQ=255)
                                 gCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAg                                                                                                                  <  6:134623/148‑1 (MQ=255)
                                  ccTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGtt                                                                                                                <  6:164589/149‑1 (MQ=255)
                                  ccTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGtt                                                                                                                <  5:368596/149‑1 (MQ=255)
                                   cTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGtt                                                                                                                >  4:23352/1‑148 (MQ=255)
                                                 ggTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGc                                                                                                 <  6:349742/149‑1 (MQ=255)
                                                   tCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCa                                                                                               <  8:114467/149‑1 (MQ=255)
                                                     gcgcATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCAt                                                                                              <  1:38690/148‑1 (MQ=255)
                                                     gcgcATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCAt                                                                                              >  3:285745/1‑148 (MQ=255)
                                                             aTTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCt                                                                                     >  7:362992/1‑149 (MQ=255)
                                                                 gcgcACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGcc                                                                                 >  2:139526/1‑149 (MQ=255)
                                                                       cGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGCCCCGGCCATTATACGGCTCGCCTGCTa                                                                            >  7:186501/1‑148 (MQ=255)
                                                                       cGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTAc                                                                           <  3:23352/149‑1 (MQ=255)
                                                                          cacaCCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGa                                                                        <  8:165103/149‑1 (MQ=255)
                                                                             acCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAAcc                                                                     <  6:43190/149‑1 (MQ=255)
                                                                                   ttCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAgtgt                                                                <  5:240472/148‑1 (MQ=255)
                                                                                      cGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTTTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTg                                                             <  8:360339/148‑1 (MQ=255)
                                                                                      cGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTg                                                             <  3:84743/148‑1 (MQ=255)
                                                                                        ccAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTaaa                                                                                                                          >  4:321979/1‑85 (MQ=255)
                                                                                          agcagGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAgt                                                                  >  2:252029/1‑139 (MQ=255)
                                                                                          agcagGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAgt                                                                  <  1:252029/139‑1 (MQ=255)
                                                                                          agcagGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTgg                                                            <  3:251362/145‑1 (MQ=255)
                                                                                          agcagGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTgg                                                            >  4:251362/1‑145 (MQ=255)
                                                                                              ggTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGc                                                    <  6:338891/149‑1 (MQ=255)
                                                                                                tttGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCgg                                                  <  8:21867/149‑1 (MQ=255)
                                                                                                        ggcggTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGcc                                          <  4:285745/149‑1 (MQ=255)
                                                                                                              gCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGCCCCGGCCTTTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCa                                    >  3:279159/1‑149 (MQ=255)
                                                                                                                            cgccCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTc                      <  7:9368/149‑1 (MQ=255)
                                                                                                                            cgccCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTc                      <  1:139526/149‑1 (MQ=255)
                                                                                                                              gactgAACAGCAACCTGTTGCGGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTc                      >  5:219096/5‑147 (MQ=255)
                                                                                                                              cccAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTc                      >  3:111350/1‑147 (MQ=255)
                                                                                                                                cAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAg                           >  1:69565/1‑140 (MQ=255)
                                                                                                                                cAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAg                           <  2:69565/140‑1 (MQ=255)
                                                                                                                                 tgAACAGCAACCTGTTGCGGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTc                      >  1:50663/2‑144 (MQ=255)
                                                                                                                                     ccGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAg             >  4:369956/1‑149 (MQ=255)
                                                                                                                                      cGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGACATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATagag             >  3:374837/1‑148 (MQ=255)
                                                                                                                                                 gCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCAcgc  <  7:124288/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGATAGCGCGGATTCTCGCGATTCACACGCATGGCCTCGGCCTGCGCCGGGTCGCGCATCCATTGGCGCACCGGCACACCGTTTTCCGCCAGCAGGTTTGCCACGGCGGTGCCGAAGCTGCCGCCGCCCAGAACCGCAACAGGTTGCTGTTCAGTCATATCCAATCCATTAAAGCCAAAAGTTAAGTGGCGACCCGGCCATTATACGGCTCGCCTGCTACAGAACCAGTGTCTGGACGTTTGCGGTACTTGCCATGGCATTTTCGGAGCCGTCGAGATCGAGCACCGCCACGC  >  NC_002947/4711384‑4711676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑