Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,729,570 T→A 6.8% N89I (AAC→ATC)  sucC ← bifunctional succinyl‑CoA synthetase subunit beta/glutaryl‑CoA synthetase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,729,5700TA 6.8% 173.9 / 2.4 59N89I (AAC→ATC) sucCbifunctional succinyl‑CoA synthetase subunit beta/glutaryl‑CoA synthetase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (25/30);  new base A (2/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

TGAGCGACTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGTGAACCTGGGCTTTTACAACCCACTCGTTCCC  >  NC_002947/4729423‑4729718
                                                                                                                                                   |                                                                                                                                                    
tGAGCGACTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGtt                                                                                                                                                     >  6:313401/1‑149 (MQ=255)
tGAGCGACTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGtt                                                                                                                                                     <  4:257473/149‑1 (MQ=255)
tGAGCGACTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGtt                                                                                                                                                     <  5:313401/149‑1 (MQ=255)
tGAGAGTCTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGTCTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGtt                                                                                                                                                     <  5:247543/149‑1 (MQ=255)
  aGCGACTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCTTGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTgg                                                                                                                                                   <  4:59388/149‑1 (MQ=255)
  aGCGACTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTg                                                                                                                                                    <  1:14363/148‑1 (MQ=255)
  aGCGACTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTg                                                                                                                                                    <  6:315782/148‑1 (MQ=255)
     gACTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGCAGCTTGGAGACGGGTTGACCGTTGGCAt                                                                                                                                                >  3:204981/1‑149 (MQ=255)
       cTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCg                                                                                                                                              <  5:308113/149‑1 (MQ=255)
       cTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCg                                                                                                                                              <  3:254926/149‑1 (MQ=255)
          tctcGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGATGCCAACGGt                                                                                                                                            >  8:222619/1‑148 (MQ=255)
            tcGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATc                                                                                                                                               <  7:265599/143‑1 (MQ=255)
            tcGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATc                                                                                                                                               >  8:265599/1‑143 (MQ=255)
              gATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTggt                                                                                                                                       <  7:118642/149‑1 (MQ=255)
              gATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTggt                                                                                                                                       <  1:193068/149‑1 (MQ=255)
              gATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTgg                                                                                                                                        >  2:139540/1‑148 (MQ=255)
              gATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTg                                                                                                                                         <  2:372225/147‑1 (MQ=255)
              gATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGATGCCAACGGTcaacc                                                                                                                                       <  7:222619/149‑5 (MQ=255)
                     aCGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAAc                                                                                                                                >  3:150651/1‑149 (MQ=255)
                         cACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAAc                                                                                                                                >  2:297684/1‑145 (MQ=255)
                            cTTCGGTGGAAGCCATGAACACGATACGGCGTCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGTCGATTTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTc                                                                                                                         <  5:159/149‑1 (MQ=255)
                            cTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTc                                                                                                                         >  5:97390/1‑149 (MQ=255)
                              tCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCtt                                                                                                                       <  3:7483/149‑1 (MQ=255)
                                ggTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCtt                                                                                                                       >  4:7483/1‑147 (MQ=255)
                                ggTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCGCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTg                                                                                                                      <  8:102226/148‑1 (MQ=255)
                                 gTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGcc                                                                                                                    >  6:116894/1‑149 (MQ=255)
                                    gAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCaa                                                                                                                 >  7:55954/1‑149 (MQ=255)
                                         cATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTggt                                                                                                                                       >  2:348394/1‑122 (MQ=255)
                                         cATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTggt                                                                                                                                       <  1:348394/122‑1 (MQ=255)
                                          aTGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCCAGCGGCAGGGTGGAGCCGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACtg                                                                                                           >  1:127241/1‑149 (MQ=255)
                                           ccaaCACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACtgt                                                                                                          <  1:139540/147‑1 (MQ=255)
                                              acacGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACtgtg                                                                                                         >  2:169042/1‑147 (MQ=255)
                                              acacGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCa                                                                                                       <  6:211395/149‑1 (MQ=255)
                                                acGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTATTGACCAACAACTGTTCAGc                                                                                                     >  4:16605/1‑149 (MQ=255)
                                                          ggcTCGACCGATCGACTCCAGCGCCCAGGGACAGCTCTTTGGCGACGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTg                                                                                           <  4:336763/149‑1 (MQ=255)
                                                               gAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCtt                                                                                                                       >  4:326195/1‑116 (MQ=255)
                                                               gAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTc                                                                                      <  1:297684/149‑1 (MQ=255)
                                                                  cGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTc                                                                                   <  5:116894/149‑1 (MQ=255)
                                                                        aCTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTtgct                                                                             >  3:311701/1‑149 (MQ=255)
                                                                            cAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCg                                                                          >  4:189929/1‑148 (MQ=255)
                                                                            cAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGa                                                                         <  2:190510/149‑1 (MQ=255)
                                                                            cAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATGCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCATCGAACGCCTTGGCGTCTTCCTtgctgc                                                                           <  6:182111/147‑1 (MQ=255)
                                                                              gcgcCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAAc                                                                       >  3:189695/1‑149 (MQ=255)
                                                                                  ccAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGc                                                                   >  3:179351/1‑149 (MQ=255)
                                                                                          aGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTAcgcc                                                           >  1:358349/1‑149 (MQ=255)
                                                                                               tttGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTCGGCGTCTTCCTtgctgc                                                                           >  8:10715/1‑128 (MQ=255)
                                                                                               tttGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTCGGCGTCTTCCTtgctgc                                                                           <  7:10715/128‑1 (MQ=255)
                                                                                               tttGGCGATGGCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACgccgcc                                                        <  8:64475/147‑1 (MQ=255)
                                                                                                   gCGAAGTGAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTCGGTATCCAGATTCTAGCCGACCCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGCACCAGCTTTACGCCGGCCGCtt                                                   >  6:231105/1‑148 (MQ=255)
                                                                                                         tCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACcgc                                             <  1:169042/148‑1 (MQ=255)
                                                                                                           aGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGa                                           <  8:55954/148‑1 (MQ=255)
                                                                                                                 ggATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCcg                                    >  5:1172/1‑149 (MQ=255)
                                                                                                                    ttCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGt                                 <  4:150651/149‑1 (MQ=255)
                                                                                                                    ttCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGt                                 <  2:358349/149‑1 (MQ=255)
                                                                                                                        aCCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGTGAAc                             <  4:189695/149‑1 (MQ=255)
                                                                                                                             aaTCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGTGAACCTggg                        <  2:127241/149‑1 (MQ=255)
                                                                                                                                   ggAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGTGAACCTGGGCtttt                   <  6:1172/148‑1 (MQ=255)
                                                                                                                                          accagACCGATGCCAACGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCtt                                                                 <  7:223931/91‑1 (MQ=255)
                                                                                                                                          accagACCGATGCCAACGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCtt                                                                 >  8:223931/5‑95 (MQ=255)
                                                                                                                                           gTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGTGAACCTGGGCTTTTACAACCCAc          >  8:311327/1‑149 (MQ=255)
                                                                                                                                              gcccGTTGTCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGTGAACCTGGGCTTTTACAACCCACTc        <  4:204981/146‑1 (MQ=255)
                                                                                                                                                 cGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGTGAACCTGGGCTTTTACAACCCACTCGTTc    >  1:88813/1‑149 (MQ=255)
                                                                                                                                                 cGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGTGAACCTGGGCTTTTACAACCCACTCGTTc    >  4:133531/1‑149 (MQ=255)
                                                                                                                                                   ttGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGTGAACCTGGGCTTTTACAACCCACTCGTTccc  >  7:37110/1‑149 (MQ=255)
                                                                                                                                                   ttGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGTGAACCTGGGCTTTTACAACCCACTCGTTccc  >  1:187083/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGAGCGACTTTCTCGATGTCCACGCCACCTTCGGTGGAAGCCATGAACACGATACGGCGGCTCGAACGATCGACTACAGCGCCCAGGTACAGCTCTTTGGCGATGTCAGTGCAGGATTCGACCAGAATCTTGGAGACGGGTTGACCGTTGGCATCGGTCTGGTAGGTAACCAGATTCTTGCCCAACCACTGTGCAGCGAACGCCTTGGCGTCTTCCTTGCTGCGAACCAGCTTTACGCCGCCCGCTTTACCGCGACCACCCGCGTGAACCTGGGCTTTTACAACCCACTCGTTCCC  >  NC_002947/4729423‑4729718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑