Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,738,324 C→T 100% G16S (GGC→AGC)  sdhA ← succinate dehydrogenase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,738,3240CT100.0% 172.7 / NA 51G16S (GGC→AGC) sdhAsuccinate dehydrogenase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/28);  total (23/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCCACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGTTGCC  >  NC_002947/4738184‑4738466
                                                                                                                                            |                                                                                                                                              
gTCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGTCGGCGCCGCCGCTACCgatg                                                                                                                                         >  1:154894/1‑148 (MQ=255)
gTCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCgatga                                                                                                                                        >  1:169997/1‑149 (MQ=255)
gTCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCgatga                                                                                                                                        >  8:170678/1‑149 (MQ=255)
    gCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCgatgatgat                                                                                                                                    <  2:354625/149‑1 (MQ=255)
    gCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGCCGGATACGCTGTGCGAACGGGTCGGCAAGACCTTGGTGACTACGGCAGTCTAGTGCCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCCATACCgatgatgat                                                                                                                                    >  3:45403/1‑149 (MQ=255)
    gCCGAAGCGATGGCGCAGGTGATGCCGCCCGGGGCGGATACGGTGTGCGAACGGGTCGTGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCgatgatgat                                                                                                                                    <  2:360290/149‑1 (MQ=255)
       gAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGc                                                                                                                                 <  7:353240/149‑1 (MQ=255)
                    aGGTGATGCCGCCCTTGGCGGATACGGTGTTCGAACGGGGCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATg                                                                                                                    <  5:221562/149‑1 (MQ=255)
                       tGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGtt                                                                                                                 <  3:21161/149‑1 (MQ=255)
                        gATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTg                                                                                                                >  7:17556/1‑149 (MQ=255)
                        gATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTg                                                                                                                >  5:318672/1‑149 (MQ=255)
                         aTGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTg                                                                                                                <  7:170678/148‑1 (MQ=255)
                           gccgccCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGaa                                                                                                                           <  4:27390/135‑1 (MQ=255)
                           gccgccCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGaa                                                                                                                           >  3:27390/1‑135 (MQ=255)
                           gccgccCTGGGCGGATACGGGGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGa                                                                                                              <  1:141651/148‑1 (MQ=255)
                            ccgccCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGaa                                                                                                             <  3:59762/148‑1 (MQ=255)
                              gccCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGc                                                                                                          <  7:179053/149‑1 (MQ=255)
                                ccTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCGGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGc                                                                                                          >  2:185695/1‑147 (MQ=255)
                                ccTGGGCGGATACGGTGTGCGAACGGGGCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTTTAATGCTa                                                                                                        <  6:368113/149‑1 (MQ=255)
                                 cTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAg                                                                                                       >  7:198166/1‑149 (MQ=255)
                                    ggCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGcc                                                                                                     <  2:368293/148‑1 (MQ=255)
                                      cGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATg                                                                                                  <  6:318672/149‑1 (MQ=255)
                                       ggATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATgg                                                                                                 <  6:129323/149‑1 (MQ=255)
                                       ggATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATgg                                                                                                 <  5:319542/149‑1 (MQ=255)
                                            cGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCa                                                                                             <  8:17556/148‑1 (MQ=255)
                                            cGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAg                                                                                            <  4:188002/149‑1 (MQ=255)
                                              gtgtGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGAt                                                                                          <  1:345936/149‑1 (MQ=255)
                                                   cGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATAcccc                                                                                     <  2:169997/149‑1 (MQ=255)
                                                    gAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATAccc                                                                                      >  2:82004/1‑147 (MQ=255)
                                                        gggTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCagag                                                                                 >  3:145732/1‑148 (MQ=255)
                                                             gggAAGAGCTTGGTGACTACGGCTGTCTTGTGGCCTCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCt                                                                            <  8:198166/148‑1 (MQ=255)
                                                                                cGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGt                                                          >  8:111260/1‑147 (MQ=255)
                                                                                 ggCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAg                                                       >  6:335024/1‑149 (MQ=255)
                                                                                    aGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGa                                                    <  3:131949/149‑1 (MQ=255)
                                                                                         tgtgGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATc                                               >  5:254357/1‑149 (MQ=255)
                                                                                             gccgccTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCaa                                           >  3:31394/1‑149 (MQ=255)
                                                                                              ccgccTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAAc                                          <  4:31394/149‑1 (MQ=255)
                                                                                               cgccTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAAcg                                         >  3:250698/1‑149 (MQ=255)
                                                                                                     gAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCa                                    >  1:203571/1‑148 (MQ=255)
                                                                                                        ccAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATAcc                                >  4:38364/1‑149 (MQ=255)
                                                                                                              gCAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTg                           <  6:162675/148‑1 (MQ=255)
                                                                                                               cAGCGCTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTgg                          <  6:254357/148‑1 (MQ=255)
                                                                                                                 gcgcTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGTAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGa                         >  3:220573/1‑147 (MQ=255)
                                                                                                                 gcgcTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATTCCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGa                         <  4:220573/147‑1 (MQ=255)
                                                                                                                 gcgcTGCGCGCATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGaa                        >  6:341745/1‑148 (MQ=255)
                                                                                                                       cgcgcATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGaaa                 <  3:38364/149‑1 (MQ=255)
                                                                                                                        gcgcATGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAAc                <  4:145732/149‑1 (MQ=255)
                                                                                                                            aTGCCGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAAt            >  6:86041/1‑149 (MQ=255)
                                                                                                                               ccGGCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCg          <  5:341745/148‑1 (MQ=255)
                                                                                                                                 ggCGCCGCCGCTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATCCCCCAGAGAATCTGCAAACCCCAGACGAAGTAACCGACCATCGCAACGCCGCATAACGCCTGGAACCGGAACCGAAACGCAg       >  3:114217/1‑149 (MQ=255)
                                                                                                                                  acgccgccgcTACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGt      >  6:217226/2‑149 (MQ=255)
                                                                                                                                    gccgccgctACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGa                                                                <  6:90611/89‑1 (MQ=255)
                                                                                                                                    gccgccgctACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGTTg    <  3:133039/149‑1 (MQ=255)
                                                                                                                                      cgccgctACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGTTGcc  <  4:250698/149‑1 (MQ=255)
                                                                                                                                       gccgctACCGATGATGATGGCGTCGAAGCAAATGGTTGGAATGCTAGCAATGGAACAGATACCCAAGAGAATCTGCGCACCCCAGACGAAGTAAGCGATCATCGCAACGCCGCAAACCGCCTGGAACAGGAAACGATTCGCAGTGGcc  >  8:35306/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                              
GTCGGCCGAAGCGATGGCGCAGGTGATGCCGCCCTGGGCGGATACGGTGTGCGAACGGGTCGGGAAGACCTTGGTGACTACGGCAGTCTTGTGGCCGCCTTGAGCCAGTTGCAGCGCTGCGCGCATGCCGGCGCCGCCGCCACCGATGATGATGGCGTCGAAGGAAATGGTTGGAATGCTAGCCATGGATCAGATACCCCAGAGAATCTGCACACCCCAGACGAAGTAAGCGAACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGTTGCC  >  NC_002947/4738184‑4738466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑