Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 62,078 A→C 9.3% E108A (GAA→GCA) ‡ PP_0053 → sulfide:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294762,0780AC 9.3% 118.3 / 5.1 43E108A (GAA→GCA) ‡PP_0053sulfide:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (22/17);  new base C (2/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.95e-01

GGTGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTCAAGCAAG  >  NC_002947/61930‑62224
                                                                                                                                                    |                                                                                                                                                  
ggTGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGa                                                                                                                                                    <  4:380516/149‑1 (MQ=255)
 gTGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGaa                                                                                                                                                   <  3:335032/149‑1 (MQ=255)
  tGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCTTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCTGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAAc                                                                                                                                                  <  8:98759/149‑1 (MQ=255)
     cGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGc                                                                                                                                               >  3:343386/1‑149 (MQ=255)
        tgtgtTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGa                                                                                                                                            >  6:169917/1‑149 (MQ=255)
            ttCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTGTTCATAGCTGAtggc                                                                                                                                        <  2:185501/149‑4 (MQ=255)
            ttCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCg                                                                                                                                         >  8:320415/1‑148 (MQ=255)
             tCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTGTTCATAGCTGAtggc                                                                                                                                        <  1:356600/148‑4 (MQ=255)
                   cACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGcccc                                                                                                                                 >  8:287970/1‑149 (MQ=255)
                    aCCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGAt                                                                                                                                           >  2:345441/1‑138 (MQ=255)
                    aCCAAGCACTGCCCGCACCATCGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGAt                                                                                                                                           <  1:345441/138‑1 (MQ=255)
                       aaGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGcc                                                                                                                             >  3:75687/1‑149 (MQ=255)
                          cACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGa                                                                                                                                                    >  2:258575/1‑123 (MQ=255)
                          cACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGa                                                                                                                                                    <  1:258575/123‑1 (MQ=255)
                                          gCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGggcggc                                                                                                          >  3:11133/1‑149 (MQ=255)
                                               gACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTg                                                                                                     <  8:348457/149‑1 (MQ=255)
                                                       cTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTc                                                                                             >  4:54187/1‑149 (MQ=255)
                                                        tCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCt                                                                                              >  3:180406/1‑147 (MQ=255)
                                                          gcgGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCa                                                                                            <  7:320415/147‑1 (MQ=255)
                                                           cgGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCg                                                                                         <  4:75687/149‑1 (MQ=255)
                                                            ggTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCga                                                                                        <  6:390565/149‑1 (MQ=255)
                                                                         tCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCACACGCTGGACTGGGCGGCCATTGATGGGATCAGCGAGACCCTGGGCCg                                                                            >  8:337499/1‑148 (MQ=255)
                                                                           aaGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCaa                                                                         <  4:343386/149‑1 (MQ=255)
                                                                                 cgGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGgtgt                                                                   <  4:11133/149‑1 (MQ=255)
                                                                                    gTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCAc                                                                >  4:23850/1‑149 (MQ=255)
                                                                                          ggATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCaa                                                          >  2:183397/1‑149 (MQ=255)
                                                                                            aTTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAAc                                                         >  6:33736/1‑148 (MQ=255)
                                                                                                gACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATc                                                     <  3:23850/148‑1 (MQ=255)
                                                                                                   ccATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGCCTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTa                                                 >  6:125127/1‑149 (MQ=255)
                                                                                                   ccATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTa                                                 >  2:274608/1‑149 (MQ=255)
                                                                                                    cATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTAc                                                <  7:287970/149‑1 (MQ=255)
                                                                                                           ccAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTgg                                         >  3:342824/1‑149 (MQ=255)
                                                                                                                  gTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCTCCCt                                   <  5:330000/148‑1 (MQ=255)
                                                                                                                  gTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGACCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCta                                  >  6:351391/1‑149 (MQ=255)
                                                                                                                               atgGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTg                     <  1:183397/149‑1 (MQ=255)
                                                                                                                                  gTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGt                   <  5:33736/148‑1 (MQ=255)
                                                                                                                                  gTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTg                  <  1:274608/149‑1 (MQ=255)
                                                                                                                                     agacgaTCAGCTGTTCATAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGCCCCTGGGCCGCAACGGTGTCCCCTCCAAATATCGCTACGACCTGGAACCCTATACCTGGGAGCTGGTGCa                >  7:275973/6‑148 (MQ=255)
                                                                                                                                      gacgaTCAGCTGTTCATAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaa               >  7:101199/5‑148 (MQ=255)
                                                                                                                                       gacATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGTGCCGCAACTGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaaaa             <  7:337499/147‑1 (MQ=255)
                                                                                                                                             aGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTg                     <  8:321823/135‑1 (MQ=255)
                                                                                                                                             aGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTg                     >  7:321823/1‑135 (MQ=255)
                                                                                                                                               cTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTcaag      >  1:181368/1‑148 (MQ=255)
                                                                                                                                                tATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTcaagc     >  3:163453/1‑148 (MQ=255)
                                                                                                                                                 aTGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaaaa             <  6:11756/139‑1 (MQ=255)
                                                                                                                                                 aTGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaaaa             >  5:11756/1‑139 (MQ=255)
                                                                                                                                                   gAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTcaagcaag  >  1:335043/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGTGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTCAAGCAAG  >  NC_002947/61930‑62224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑