Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,796,619 A→G 16.3% intergenic (‑177/+41) pvdH ← / ← PP_4224 diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,796,6190AG16.3% 107.4 / 13.6 49intergenic (‑177/+41)pvdH/PP_4224diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (22/19);  new base G (5/3);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

GAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCC  >  NC_002947/4796471‑4796756
                                                                                                                                                    |                                                                                                                                              
gAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGa                                                                                                                                                <  6:193202/149‑1 (MQ=255)
     ggTGGACATGGGTAAATCCTCGCGAGCAAGGGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTAcgggcggg                                                                                                                                           <  2:12548/149‑1 (MQ=38)
      gTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGt                                                                                                                                          >  7:287925/1‑149 (MQ=255)
      gTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCATCTTCGAGGGTAAACCGGCCCGTAGGAGCGGGt                                                                                                                                          >  7:265120/1‑149 (MQ=255)
         gACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTc                                                                                                                                        <  7:67657/148‑1 (MQ=255)
          aCATGGGTAAATCCTCCCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAc                                                                                                                                      <  6:251067/149‑1 (MQ=38)
            aTGGGTATATGCTCGCGAGCAAGCGGATGGGCGGTTTTCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAccc                                                                                                                                    <  8:73390/149‑1 (MQ=37)
               ggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc                                                                                                                                  <  4:21401/148‑1 (MQ=255)
               ggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc                                                                                                                                  <  4:242600/148‑1 (MQ=255)
                 tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgcg                                                                                                                                 >  8:186667/1‑147 (MQ=255)
                 tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgcg                                                                                                                                 >  6:150099/1‑147 (MQ=255)
                 tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCGTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGaa                                                                                                                               <  8:351853/149‑1 (MQ=255)
                       cTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc                                                                                                                          <  5:150099/148‑1 (MQ=255)
                       cTCGCGAGCAAGAGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc                                                                                                                          <  4:131950/148‑1 (MQ=255)
                        tCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc                                                                                                                          >  2:122020/1‑147 (MQ=255)
                               cAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAg‑‑g                                                                                                               <  8:287925/149‑1 (MQ=255)
                                aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGc                                                                                                                                              <  3:335646/119‑1 (MQ=255)
                                aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGc                                                                                                                                              >  4:335646/1‑119 (MQ=255)
                                            ggTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑Gc                                                                                                              >  4:83504/1‑137 (MQ=255)
                                            ggTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑Gc                                                                                                              <  3:83504/137‑1 (MQ=255)
                                                aTCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGcc                                                                                           >  6:284067/1‑149 (MQ=255)
                                                       tGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGa                                                                                      <  2:103185/147‑1 (MQ=255)
                                                        ggAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGAcgc                                                                                   >  4:366899/1‑149 (MQ=255)
                                                            aCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGAGGGTAAACCCGCCCGTAGGATCGGGTTAACCCGAGAAGACGCCAGCAAAG‑‑GCGATAAACAT‑‑‑CAAAGCCCATGACGCGCCg                                                                               >  5:85342/1‑149 (MQ=255)
                                                              gCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAaga                                                                                                                             >  4:75250/1‑106 (MQ=18)
                                                              gCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAaga                                                                                                                             <  3:75250/106‑1 (MQ=18)
                                                                           gaggagTTAGCGGTTGTTACGCGATTTAATTTTGGTCTGTGCTGCCCTCTTCGCGGGTAAACCCGCCCGTCGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACaaaa                                                                <  4:191611/149‑1 (MQ=38)
                                                                               aTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAAACCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGa                                                                                                            >  1:169276/1‑104 (MQ=255)
                                                                                                 gATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCt                                          <  5:284067/149‑1 (MQ=255)
                                                                                                    tttattttTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGt                                        >  2:238468/1‑148 (MQ=255)
                                                                                                        tgtttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa                                    <  8:265120/146‑1 (MQ=255)
                                                                                                         atttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACCT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACCAAACCCGCAAGCCGTCACTACGGCTGTCAAAgc                                  >  7:44262/2‑149 (MQ=255)
                                                                                                              tCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAg                              <  7:186667/148‑1 (MQ=255)
                                                                                                                  tgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGc                            <  3:366899/146‑1 (MQ=255)
                                                                                                                  tgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTAc                         >  5:299712/1‑149 (MQ=255)
                                                                                                                      ggCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCgg                      >  8:99483/1‑148 (MQ=255)
                                                                                                                         ctctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGCCGCGATAAACATACCCCTTGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCg                       <  1:122020/149‑1 (MQ=37)
                                                                                                                          tctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCacac                 >  8:194003/1‑149 (MQ=255)
                                                                                                                          tacgggcgGGTTTACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCacac                 >  7:353813/6‑149 (MQ=255)
                                                                                                                                 gggTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCa          <  5:6722/149‑1 (MQ=255)
                                                                                                                                   gtgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc         >  5:73550/4‑148 (MQ=38)
                                                                                                                                   gTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACAGCTGCAc         >  6:37386/1‑148 (MQ=255)
                                                                                                                                    tgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCGGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGa       >  1:279036/3‑149 (MQ=38)
                                                                                                                                        cccgctccTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc   >  2:289575/1‑149 (MQ=38)
                                                                                                                                        cccgctccTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc   >  3:205872/1‑149 (MQ=38)
                                                                                                                                         ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGcc  >  6:349100/1‑149 (MQ=255)
                                                                                                                                         ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGcc  >  1:341868/1‑149 (MQ=255)
                                                                                                                                           gcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc         <  2:249351/140‑1 (MQ=255)
                                                                                                                                           gcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc         >  1:249351/1‑140 (MQ=255)
                                                                                                                                                   gAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa                                    <  3:294167/105‑1 (MQ=255)
                                                                                                                                                   gAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa                                    >  4:294167/1‑105 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCC  >  NC_002947/4796471‑4796756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑