| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,796,627 | C→T | 19.8% | intergenic (‑185/+33) | pvdH ← / ← PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,796,627 | 0 | C | T | 19.8% | 91.7 / 14.8 | 46 | intergenic (‑185/+33) | pvdH/PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base C (19/17); new base T (5/4); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.02e-01 | |||||||||||
GACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCC > NC_002947/4796480‑4796773 | gACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTc < 7:67657/148‑1 (MQ=255) aCATGGGTAAATCCTCCCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAc < 6:251067/149‑1 (MQ=38) aTGGGTATATGCTCGCGAGCAAGCGGATGGGCGGTTTTCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAccc < 8:73390/149‑1 (MQ=37) ggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc < 4:21401/148‑1 (MQ=255) ggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc < 4:242600/148‑1 (MQ=255) tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgcg > 8:186667/1‑147 (MQ=255) tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgcg > 6:150099/1‑147 (MQ=255) tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCGTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGaa < 8:351853/149‑1 (MQ=255) cTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc < 5:150099/148‑1 (MQ=255) cTCGCGAGCAAGAGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc < 4:131950/148‑1 (MQ=255) tCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc > 2:122020/1‑147 (MQ=255) cAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAg‑‑g < 8:287925/149‑1 (MQ=255) ggTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑Gc > 4:83504/1‑137 (MQ=255) ggTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑Gc < 3:83504/137‑1 (MQ=255) aTCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGcc > 6:284067/1‑149 (MQ=255) tGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGa < 2:103185/147‑1 (MQ=255) ggAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGAcgc > 4:366899/1‑149 (MQ=255) aCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGAGGGTAAACCCGCCCGTAGGATCGGGTTAACCCGAGAAGACGCCAGCAAAG‑‑GCGATAAACAT‑‑‑CAAAGCCCATGACGCGCCg > 5:85342/1‑149 (MQ=255) gCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAaga > 4:75250/1‑106 (MQ=18) gCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAaga < 3:75250/106‑1 (MQ=18) gaggagTTAGCGGTTGTTACGCGATTTAATTTTGGTCTGTGCTGCCCTCTTCGCGGGTAAACCCGCCCGTCGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACaaaa < 4:191611/149‑1 (MQ=38) aTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAAACCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGa > 1:169276/1‑104 (MQ=255) gATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCt < 5:284067/149‑1 (MQ=255) tttattttTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGt > 2:238468/1‑148 (MQ=255) tgtttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa < 8:265120/146‑1 (MQ=255) atttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACCT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACCAAACCCGCAAGCCGTCACTACGGCTGTCAAAgc > 7:44262/2‑149 (MQ=255) tCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAg < 7:186667/148‑1 (MQ=255) tgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGc < 3:366899/146‑1 (MQ=255) tgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTAc > 5:299712/1‑149 (MQ=255) ggCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCgg > 8:99483/1‑148 (MQ=255) ctctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGCCGCGATAAACATACCCCTTGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCg < 1:122020/149‑1 (MQ=37) tctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCacac > 8:194003/1‑149 (MQ=255) tacgggcgGGTTTACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCacac > 7:353813/6‑149 (MQ=255) gggTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCa < 5:6722/149‑1 (MQ=255) gtgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc > 5:73550/4‑148 (MQ=38) gTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACAGCTGCAc > 6:37386/1‑148 (MQ=255) tgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCGGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGa > 1:279036/3‑149 (MQ=38) cccgctccTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc > 2:289575/1‑149 (MQ=38) cccgctccTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc > 3:205872/1‑149 (MQ=38) ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGcc > 6:349100/1‑149 (MQ=255) ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGcc > 1:341868/1‑149 (MQ=255) gcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc < 2:249351/140‑1 (MQ=255) gcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc > 1:249351/1‑140 (MQ=255) ctacgggCGGGTTTACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGca < 1:289575/141‑1 (MQ=255) gAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa > 4:294167/1‑105 (MQ=255) gAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa < 3:294167/105‑1 (MQ=255) tCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCgcccgccc > 4:120167/1‑148 (MQ=255) | GACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAG‑‑GCGATAAACAT‑‑‑CAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCCAGCCC > NC_002947/4796480‑4796773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |